Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,214,339 (C)6→5 coding (306/1686 nt) yehU ← inner membrane putative sensory kinase in two‑component system with YehT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,214,3340C.98.4% 243.7 / ‑2.4 61coding (311/1686 nt)yehUinner membrane putative sensory kinase in two‑component system with YehT
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (29/31);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACC  >  NC_000913/2214196‑2214463
                                                                                                                                          |                                                                                                                                 
gCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGc                                                                                                                                   >  2:1544653/1‑139 (MQ=255)
  gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCTGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATcccc                                                                                                                                 <  1:1325771/139‑1 (MQ=255)
  gacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  2:304788/139‑1 (MQ=255)
     gaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                              <  2:1771321/139‑2 (MQ=255)
     gaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                              <  2:872252/139‑2 (MQ=255)
     gaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCACGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccccca                                                                                                                              >  1:1266068/1‑138 (MQ=255)
      aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccat                                                                                                                             >  1:758961/1‑137 (MQ=255)
      aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcccccat                                                                                                                             >  1:987428/1‑137 (MQ=255)
         gTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGa                                                                                                                         <  2:987428/139‑1 (MQ=255)
                         tAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAgccg                                                                                                         <  1:2035856/139‑1 (MQ=255)
                             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     <  1:116246/139‑1 (MQ=255)
                                  tAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                <  2:1495537/139‑1 (MQ=255)
                                  tAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                <  2:1266068/139‑1 (MQ=255)
                                       aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           <  2:583824/139‑1 (MQ=255)
                                          ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                        >  2:1212060/1‑139 (MQ=255)
                                          ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                        >  2:755803/1‑139 (MQ=255)
                                           tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     <  2:1168473/125‑1 (MQ=255)
                                           tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     >  1:1168473/1‑125 (MQ=255)
                                                 tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGTCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcc                                                                                                                                  >  1:28268/1‑91 (MQ=255)
                                                 tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGTCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGcc                                                                                                                                  <  2:28268/91‑1 (MQ=255)
                                                   cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgac                                                                               >  1:23581/1‑139 (MQ=255)
                                                     cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                >  2:928605/1‑120 (MQ=255)
                                                     cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGc                                                                                                <  1:928605/120‑1 (MQ=255)
                                                      ccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCgacgac                                                                            >  2:580050/1‑139 (MQ=255)
                                                           gcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggac                                                                       >  2:1254100/1‑139 (MQ=255)
                                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            <  1:261816/139‑1 (MQ=255)
                                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                            <  1:755803/139‑1 (MQ=255)
                                                                        cTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgcc                                                          >  1:852015/1‑139 (MQ=255)
                                                                         tgtgTACCAGGCCACCGAGTAATCCTTTCACGATGGTGGAGATCATGCAAATTAACACGGGCATTCCCCCCTCGAAAATCGATTTAAGCCCCCCGGCAGCCCCACCCGCCCACCGACGACCGGACCGCCGAGTAAaccc                                                         >  1:903357/1‑138 (MQ=255)
                                                                           tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                       >  1:1817777/1‑139 (MQ=255)
                                                                              aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  1:1130718/63‑1 (MQ=255)
                                                                              aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 >  2:1130718/1‑63 (MQ=255)
                                                                              aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     <  1:529960/90‑1 (MQ=255)
                                                                              aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCgg                                                                                                     >  2:529960/1‑90 (MQ=255)
                                                                                 aGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAc                                                 <  1:1089122/139‑1 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           <  1:14948/96‑1 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                           >  2:14948/1‑96 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                >  2:1123360/1‑139 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                <  2:903357/139‑1 (MQ=255)
                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                <  2:509423/139‑1 (MQ=255)
                                                                                     cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                             >  1:1734203/1‑139 (MQ=255)
                                                                                     cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                             >  2:1274510/1‑139 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                           >  2:1950266/1‑139 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                           >  2:1039660/1‑139 (MQ=255)
                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                           <  1:1039660/139‑1 (MQ=255)
                                                                                        cGAGTAATCTTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCt                                          <  1:1212060/139‑1 (MQ=255)
                                                                                          aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAc                                                 >  2:2025112/1‑130 (MQ=255)
                                                                                          aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAc                                                 <  1:2025112/130‑1 (MQ=255)
                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  2:1734203/139‑1 (MQ=255)
                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  1:1544653/139‑1 (MQ=255)
                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  1:1950266/139‑1 (MQ=255)
                                                                                                    cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 >  2:1074092/1‑41 (MQ=255)
                                                                                                    cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGccc                                                                                                                                 <  1:1074092/41‑1 (MQ=255)
                                                                                                    cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    >  2:1672467/1‑133 (MQ=255)
                                                                                                    cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  1:1672467/133‑1 (MQ=255)
                                                                                                    cAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                              >  2:420178/1‑139 (MQ=255)
                                                                                                      aCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                               <  2:865698/120‑1 (MQ=255)
                                                                                                      aCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                               >  1:865698/1‑120 (MQ=255)
                                                                                                      aCGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                            <  1:580050/139‑1 (MQ=255)
                                                                                                        gATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTgg                          <  1:1123360/139‑1 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    >  2:1497582/1‑127 (MQ=255)
                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                    <  1:1497582/127‑1 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                       >  2:420908/1‑96 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                       <  1:420908/96‑1 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                           >  2:148378/1‑108 (MQ=255)
                                                                                                                      tGCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                           <  1:148378/108‑1 (MQ=255)
                                                                                                                       gCAACTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCaa           >  2:1937981/1‑139 (MQ=255)
                                                                                                                           cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg       >  2:835781/1‑139 (MQ=255)
                                                                                                                           cTTAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg       <  2:23581/139‑1 (MQ=255)
                                                                                                                            ttAACGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGc      >  2:1003069/1‑139 (MQ=255)
                                                                                                                              aaCGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa    <  2:758961/139‑1 (MQ=255)
                                                                                                                              aaCGCGGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa    >  1:1397673/1‑139 (MQ=255)
                                                                                                                                cgcgGTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAAcc  >  2:1727930/1‑139 (MQ=255)
                                                                                                                                    gTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                             <  1:240298/60‑1 (MQ=255)
                                                                                                                                    gTCATGCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                             >  2:240298/1‑60 (MQ=255)
                                                                                                                                          |                                                                                                                                 
GCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATGCCCCCCATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACC  >  NC_000913/2214196‑2214463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: