Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,742,599 Δ1 bp intergenic (‑67/‑134) yiaJ ← / → yiaK transcriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,742,5980C.100.0% 102.5 / NA 26intergenic (‑66/‑135)yiaJ/yiaKtranscriptional repressor for the yiaKLMNO‑lyxK‑sgbHUE operon/2,3‑diketo‑L‑gulonate reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/14);  total (12/14)

GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATTGCCTTGT  >  NC_000913/3742463‑3742645
                                                                                                                                       |                                               
ggCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              >  1:301760/1‑136 (MQ=255)
          tGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTAt                                   <  2:301760/139‑1 (MQ=255)
                 tctTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCtttt                            >  2:1028539/1‑139 (MQ=255)
                  ctTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTg                           <  1:479565/139‑1 (MQ=255)
                   tttttCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGa                          >  2:1550356/1‑139 (MQ=255)
                     tttCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGaaa                        <  1:1028539/139‑1 (MQ=255)
                     tttCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGaaa                        <  2:945050/139‑1 (MQ=255)
                           gcgcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTAt                                   <  1:452501/122‑1 (MQ=255)
                           gcgcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatc                  >  1:477475/1‑139 (MQ=255)
                           gcgcCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAAAATTGATTTTTAt                                   >  2:452501/1‑122 (MQ=255)
                               gaTCTCGTTCTCTTTTTTTCCCATCCCTTCCTTCCCCATTTTGTCGCGTCCTGATGGTACCGCAAAGTGTGCCGTAGTTCACGATCTCGACACATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatc              <  1:736224/138‑1 (MQ=255)
                                    cGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  1:1550356/139‑6 (MQ=255)
                                    cGTTCTCATTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  1:680886/139‑6 (MQ=255)
                                           tttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgacaatt  >  1:1553160/1‑127 (MQ=255)
                                           tttttttCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgacaatc  >  2:736224/1‑127 (MQ=255)
                                                    cATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         >  1:180371/1‑118 (MQ=255)
                                                    cATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgtt         <  2:180371/123‑6 (MQ=255)
                                                                      tttGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatc              <  1:360853/100‑1 (MQ=255)
                                                                      tttGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatc              >  2:360853/1‑100 (MQ=255)
                                                                               tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              <  1:1163974/60‑4 (MQ=255)
                                                                               tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              >  2:1163974/1‑57 (MQ=255)
                                                                               tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgac      >  1:745456/1‑91 (MQ=255)
                                                                               tCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATTCatcaatcctgttgac      <  2:745456/99‑9 (MQ=255)
                                                                                              aaaGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGaaa                        <  1:934446/66‑1 (MQ=255)
                                                                                              aaaGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGaaa                        >  2:934446/1‑66 (MQ=255)
                                                                                                aGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              >  2:1385307/1‑40 (MQ=255)
                                                                                                aGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAAcaat                                              <  1:1385307/43‑4 (MQ=255)
                                                                                                aGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 <  2:1588192/71‑1 (MQ=255)
                                                                                                aGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 >  1:1588192/1‑71 (MQ=255)
                                                                                                            ttCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 >  2:1992286/1‑59 (MQ=255)
                                                                                                            ttCACGATCTCGACAGATAATTTATAACAATTGATTTTTATGTCTTTTGAAATtcatca                 <  1:1992286/59‑1 (MQ=255)
                                                                                                                                       |                                               
GGCTTCCGGCTGGACGCTCTTTTTCCTGCGCCATCTCGTTCTCTTTTTTTCCCATCACTTCTTTCCCCATTTTGTCGCGTCCTGATGGTAGCGCAAAGTGTGCCGTAGTTCACGATCTCGACAGATAATTTATAACCAATTGATTTTTATGTCTTTTGAAATTCATCAATCAGATTGCCTTGT  >  NC_000913/3742463‑3742645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: