Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,330 (G)8→7 noncoding (72/87 nt) leuQ ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,3230G.97.7% 342.8 / 0.9 87noncoding (79/87 nt)leuQtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (42/43);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGT  >  NC_000913/4606187‑4606450
                                                                                                                                         |                                                                                                                               
atatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggg                                                                                                                               >  1:11634/1‑139 (MQ=255)
 tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGATACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                                                                                                                              <  2:2003437/139‑1 (MQ=255)
 tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                                                                                                                              <  1:891322/139‑1 (MQ=255)
   tCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                                                                                            <  1:983677/139‑1 (MQ=255)
           tttGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGa                                                                                                                    <  1:1207922/139‑1 (MQ=18)
               gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAAcccc                                                                                                               <  1:156715/139‑1 (MQ=255)
                tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAAccccc                                                                                                              >  1:804736/1‑139 (MQ=255)
                           gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                             >  2:943807/1‑130 (MQ=255)
                           gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                             <  1:943807/130‑1 (MQ=255)
                           gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTaa                                                                                                    >  2:942982/1‑139 (MQ=255)
                            ggACTTGATCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                   <  2:674526/139‑1 (MQ=255)
                            ggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                   <  1:385905/139‑1 (MQ=255)
                                  gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                             >  2:1682019/1‑139 (MQ=255)
                                  gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                             <  1:1697692/139‑1 (MQ=255)
                                    aCCCCCACTTTCGTAAGGACACTAGCACCTGAAGCTAGCGCGTCGACCGATACCGTCACCAGCGTCCAGTGCGAATAAGATCAAGGTGGTTTTTGGTGCGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                           <  1:156516/139‑1 (MQ=21)
                                    aCCCCCACGTCCGTAAGGACACTAACGCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                           <  2:1089716/139‑1 (MQ=255)
                                        ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCATTACGAGCACTATCAACGTAGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                       >  1:1155364/1‑139 (MQ=25)
                                        ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                       >  2:660421/1‑139 (MQ=255)
                                         cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                                                      <  2:804736/139‑1 (MQ=255)
                                             tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggac                                                                                                                         <  2:1601544/101‑3 (MQ=255)
                                             tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggggac                                                                                                                         >  1:1601544/1‑99 (MQ=255)
                                               cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                                <  2:1611322/139‑1 (MQ=255)
                                                gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                               <  2:11634/139‑1 (MQ=255)
                                                     gacacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                          <  1:660421/138‑1 (MQ=255)
                                                      acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                                                         >  1:315203/1‑139 (MQ=255)
                                                        acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                             <  2:782649/101‑1 (MQ=255)
                                                        acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                             >  1:782649/1‑101 (MQ=255)
                                                        acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTc                                                                                                         <  2:11901/105‑1 (MQ=255)
                                                        acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTc                                                                                                         >  1:11901/1‑105 (MQ=255)
                                                        acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                       >  1:1611322/1‑139 (MQ=255)
                                                        acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                       >  1:1796241/1‑139 (MQ=255)
                                                          tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                                                                                                                              >  1:1967037/1‑83 (MQ=255)
                                                          tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggg                                                                                                                              <  2:1967037/83‑1 (MQ=255)
                                                          tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                             >  1:569042/1‑115 (MQ=255)
                                                          tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                             <  2:569042/115‑1 (MQ=255)
                                                          tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                                                                     >  1:775431/1‑139 (MQ=255)
                                                            acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                   <  1:1682019/139‑1 (MQ=255)
                                                                  gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                             >  2:909937/1‑139 (MQ=255)
                                                                   aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                            >  2:1280838/1‑139 (MQ=255)
                                                                   aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                            <  2:775431/139‑1 (MQ=255)
                                                                   aaGCTAGCGCGTCTACCAATTCCGCCACCTACGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                            <  2:1573980/139‑1 (MQ=255)
                                                                        aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                            <  1:247060/134‑1 (MQ=255)
                                                                        aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                            >  2:247060/1‑134 (MQ=255)
                                                                        aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                      >  2:792385/1‑139 (MQ=255)
                                                                          cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTg                                                                                                                     >  2:4957/1‑75 (MQ=255)
                                                                          cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTg                                                                                                                     <  1:4957/75‑1 (MQ=255)
                                                                             gTCTACCAATTCCGCCACCTTCGCCCAGTGCGATCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                  <  1:319273/139‑1 (MQ=255)
                                                                              tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                 <  1:1280838/139‑1 (MQ=255)
                                                                              tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                 <  2:315203/139‑1 (MQ=255)
                                                                                         cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc                                      >  1:1156497/1‑139 (MQ=255)
                                                                                         cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc                                      >  2:1758684/1‑139 (MQ=255)
                                                                                           ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                                    >  2:568495/1‑139 (MQ=255)
                                                                                           ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                                    >  1:1628408/1‑139 (MQ=255)
                                                                                            cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                                  >  2:1984127/1‑139 (MQ=255)
                                                                                              ccTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaa                                 <  2:1796241/139‑1 (MQ=255)
                                                                                                 tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTc                                                                                                         >  1:910438/1‑64 (MQ=255)
                                                                                                 tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTc                                                                                                         <  2:910438/64‑1 (MQ=255)
                                                                                                  cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                          <  2:1217155/110‑1 (MQ=255)
                                                                                                  cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                          >  1:1217155/1‑110 (MQ=255)
                                                                                                  cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                             <  1:942982/139‑1 (MQ=255)
                                                                                                        gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGa                                                                                                                    <  2:1368554/46‑1 (MQ=38)
                                                                                                        gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGa                                                                                                                    >  1:1368554/1‑46 (MQ=38)
                                                                                                        gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAAccccc                                                                                                              <  2:1429385/52‑1 (MQ=255)
                                                                                                        gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAAccccc                                                                                                              >  1:1429385/1‑52 (MQ=255)
                                                                                                         tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                             <  2:1546890/52‑1 (MQ=255)
                                                                                                         tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                             >  1:1546890/1‑52 (MQ=255)
                                                                                                         tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                           >  2:1535935/1‑102 (MQ=255)
                                                                                                         tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                           <  1:1535935/102‑1 (MQ=255)
                                                                                                         tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                      >  2:1966202/1‑139 (MQ=255)
                                                                                                          gCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc                     <  1:1758684/139‑1 (MQ=255)
                                                                                                              gCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                 <  2:1156497/139‑1 (MQ=255)
                                                                                                               cAATATCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACgg                <  1:1966202/139‑1 (MQ=255)
                                                                                                                   atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                       <  1:442223/64‑1 (MQ=255)
                                                                                                                   atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                       >  2:442223/1‑64 (MQ=255)
                                                                                                                   atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                  >  1:434682/1‑101 (MQ=255)
                                                                                                                   atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                  <  2:434682/101‑1 (MQ=255)
                                                                                                                   atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc            >  1:1376507/1‑139 (MQ=255)
                                                                                                                   atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc            <  2:1628408/139‑1 (MQ=255)
                                                                                                                   atCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgc            >  1:975009/1‑139 (MQ=255)
                                                                                                                    tCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg           <  2:975009/139‑1 (MQ=255)
                                                                                                                    tCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg           <  1:909937/139‑1 (MQ=255)
                                                                                                                    tCAACGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTCCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg           <  2:1376507/139‑1 (MQ=255)
                                                                                                                        cGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                    >  1:1884532/1‑94 (MQ=255)
                                                                                                                        cGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                    <  2:1884532/94‑1 (MQ=255)
                                                                                                                        cGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                 <  1:1369108/97‑1 (MQ=255)
                                                                                                                        cGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                 >  2:1369108/1‑97 (MQ=255)
                                                                                                                        cGTGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCAtt       >  2:667641/1‑139 (MQ=255)
                                                                                                                          tGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                             <  2:336933/35‑1 (MQ=17)
                                                                                                                          tGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCa                                                                                                             >  1:336933/1‑35 (MQ=17)
                                                                                                                          tGGTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt     >  2:323299/1‑139 (MQ=255)
                                                                                                                          tGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACGAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa    >  2:245380/1‑139 (MQ=255)
                                                                                                                            gTTTTTGGTGCGAGGGGGGG‑‑CTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGt  >  2:884598/1‑139 (MQ=255)
                                                                                                                            gTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                       >  2:838408/1‑55 (MQ=255)
                                                                                                                            gTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                       <  1:838408/55‑1 (MQ=255)
                                                                                                                            gTTTTTGGTGCGAGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg   >  1:712467/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                               
ATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGT  >  NC_000913/4606187‑4606450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: