| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,920,205 | (T)7→8 | intergenic (+62/‑18) | yebT → / → rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,920,198 | 1 | . | T | 97.3% | 230.6 / ‑1.1 | 73 | intergenic (+55/‑25) | yebT/rsmF | MCE domain protein/16S rRNA m(5)C1407 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base T (37/34); total (38/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
GGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920068‑1920330 | ggAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑tttttttt > 1:1202598/1‑138 (MQ=255) cAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGt < 2:1202598/139‑1 (MQ=255) cTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACa > 1:1855321/1‑139 (MQ=255) gCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTgg > 1:64793/1‑139 (MQ=255) aGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg > 1:700301/1‑121 (MQ=255) aGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg < 2:700301/121‑1 (MQ=255) tGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacaca > 1:1956142/1‑139 (MQ=255) gAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGc < 2:64793/139‑1 (MQ=255) gAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGc < 2:1956142/139‑1 (MQ=255) ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg < 1:1417048/89‑1 (MQ=255) ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg > 2:1417048/1‑89 (MQ=255) ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGcc > 2:441942/1‑107 (MQ=255) ggAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGcc < 1:441942/107‑1 (MQ=255) cTGCGCTTCCCAAATAATGCCCAATGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGaca < 1:1390423/139‑1 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc < 2:199144/133‑1 (MQ=255) tGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTc > 1:199144/1‑133 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg < 2:344810/97‑1 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg > 1:1672235/1‑97 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg > 1:344810/1‑97 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg < 2:1672235/97‑1 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgcc < 1:1101659/80‑5 (MQ=255) cgcTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgcc > 2:1101659/1‑76 (MQ=255) ttCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATg > 1:1068437/1‑139 (MQ=255) aaaTAATGTCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg < 1:368926/113‑1 (MQ=255) aaaTAATGTCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg > 2:368926/1‑113 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCt > 2:1971811/1‑78 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCt < 1:1971811/78‑1 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac < 2:1747047/93‑1 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAacacac > 1:1747047/1‑93 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGAt < 1:543684/138‑1 (MQ=255) gCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGAt > 2:543684/1‑138 (MQ=255) cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc > 2:1520096/1‑108 (MQ=255) cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGAc < 1:1520096/108‑1 (MQ=255) cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttt < 2:969240/60‑1 (MQ=255) cccACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttt > 1:969240/1‑60 (MQ=255) aCTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg > 2:956830/1‑106 (MQ=255) aCTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACg < 1:956830/106‑1 (MQ=255) gCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGa < 2:1855321/139‑1 (MQ=255) cGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGct < 2:1068437/139‑1 (MQ=255) cGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGcct < 2:453049/72‑2 (MQ=255) cGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGcct > 1:453049/1‑71 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAac > 1:1446314/1‑73 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAac < 2:1446314/73‑1 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGctc > 1:505319/1‑135 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGctc < 2:505319/135‑1 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAt > 1:1280692/1‑139 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAt > 1:225590/1‑139 (MQ=255) tGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAt > 2:939035/1‑139 (MQ=255) gCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCAtt < 1:939035/139‑1 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc > 2:307209/1‑43 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑ttttttttgc < 1:307209/46‑4 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTg < 1:1212536/63‑1 (MQ=255) ccTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTg > 2:1212536/1‑63 (MQ=255) cTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg < 2:1453953/48‑1 (MQ=39) cTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAg > 1:1453953/1‑48 (MQ=255) gcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGa > 2:41839/1‑85 (MQ=255) gcCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGa < 1:41839/85‑1 (MQ=255) gAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACa > 1:238708/1‑47 (MQ=255) gAGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACa < 2:238708/47‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg > 2:1633800/1‑54 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTg < 1:1633800/54‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTa > 2:472607/1‑71 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTa < 1:472607/71‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCg < 2:777671/108‑1 (MQ=255) aGCGTTTATGCTAAACTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCg > 1:777671/1‑108 (MQ=255) aaaCTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTTACACAAATgcg > 1:1012143/1‑89 (MQ=255) aaaCTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATgcg < 2:1012143/89‑1 (MQ=255) cTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGa > 2:250647/1‑103 (MQ=255) cTGCGCGCCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGa < 1:250647/103‑1 (MQ=255) cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg < 1:1520235/60‑1 (MQ=255) cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCg > 2:1520235/1‑60 (MQ=255) cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 2:350423/1‑139 (MQ=255) cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGcc > 1:612392/1‑139 (MQ=255) cgcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTAGATGATTTTCTTGCCGCCTGTCAGCGcc > 2:1234055/1‑139 (MQ=255) gcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTtgat > 2:887619/1‑113 (MQ=255) gcgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTtgat < 1:887619/113‑1 (MQ=255) cgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg > 1:1747938/1‑97 (MQ=255) cgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCg < 2:1747938/97‑1 (MQ=255) cgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCg > 2:431500/1‑139 (MQ=255) cgcCTGTTTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCg < 1:350423/139‑1 (MQ=255) | GGAAGGCAAACACTTCCTGTTGCAGGAAAGTGAACCGAAAGAGTGGCGTGAATGGGGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTG‑TTTTTTTGCCAGTGGTACATGCTCGTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGATGATTTTCTTGCCGCCTGTCAGCGCCCG > NC_000913/1920068‑1920330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |