Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,484,897 (C)6→7 coding (524/3594 nt) evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,484,8911.C97.9% 328.6 / ‑0.9 96coding (518/3594 nt)evgShybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (48/46);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

Only 100 of 118 total aligned reads displayed.

CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG  >  NC_000913/2484753‑2485023
                                                                                                                                           |                                                                                                                                     
cTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        <  1:258689/139‑1 (MQ=255)
     ccgccTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                                 >  2:439476/1‑139 (MQ=255)
                taatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGt                                                                                                                       <  2:890576/139‑1 (MQ=255)
                taatGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGt                                                                                                                       <  2:351612/139‑1 (MQ=255)
                   tGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAAt                                                                                                                    >  1:1180032/1‑139 (MQ=255)
                      cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATtca                                                                                                                 >  2:1058299/1‑139 (MQ=255)
                      cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                                 <  2:764212/122‑1 (MQ=255)
                      cATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccc                                                                                                                                 >  1:764212/1‑122 (MQ=255)
                           ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTaa                                                                                                                     <  1:1035659/130‑1 (MQ=255)
                           ctgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTaa                                                                                                                     >  2:1035659/1‑130 (MQ=255)
                            tgcAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCatcaatca                                                                                                          <  2:1136380/139‑1 (MQ=255)
                              cAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCAt                                                                                                         >  1:385379/1‑139 (MQ=255)
                                   aaaccaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCa                                                                                                    <  2:1247316/139‑1 (MQ=255)
                                     accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTaa                                                                                                                     >  2:421011/1‑120 (MQ=255)
                                     accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                            >  1:1005584/1‑108 (MQ=255)
                                     accaTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAccccccc                                                                                                                               >  1:1331642/1‑108 (MQ=255)
                                       caTTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAg                                                                                                <  2:862918/139‑1 (MQ=255)
                                                ttACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCGCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTa                                                                                       >  1:1474457/1‑139 (MQ=255)
                                                 tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               <  1:1434353/130‑1 (MQ=255)
                                                 tACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               >  2:1434353/1‑130 (MQ=255)
                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTaa                                                                                                                     <  2:1629580/103‑1 (MQ=255)
                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTaa                                                                                                                     >  1:1629580/1‑103 (MQ=255)
                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtt                                                                                 >  2:90968/1‑139 (MQ=255)
                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                      <  2:1863529/86‑1 (MQ=255)
                                                      ttCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                      >  1:1863529/1‑86 (MQ=255)
                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        <  1:303873/84‑1 (MQ=255)
                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        >  2:303873/1‑84 (MQ=255)
                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCttt                                                                                >  2:130356/1‑139 (MQ=255)
                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                            <  2:1468520/94‑5 (MQ=255)
                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                            <  2:1523010/94‑5 (MQ=255)
                                                       tCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                            >  1:1523010/1‑90 (MQ=255)
                                                        ccGGCGCTGGTAACCACCCTTCACGACTGAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCCCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCtttt                                                                               >  2:1275776/1‑139 (MQ=255)
                                                                 gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               >  1:1720136/1‑114 (MQ=255)
                                                                 gTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGc                                                                                               <  2:1720136/114‑1 (MQ=255)
                                                                    accaccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTatat                                                                   <  1:2007943/139‑1 (MQ=255)
                                                                      caccCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCa                                                                 <  2:1077462/139‑1 (MQ=255)
                                                                           ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            <  1:90968/139‑1 (MQ=255)
                                                                           ttCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            >  1:147820/1‑139 (MQ=255)
                                                                                     aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa                                                                                                  <  1:240472/91‑1 (MQ=255)
                                                                                     aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCaaa                                                                                                  >  2:240472/1‑91 (MQ=255)
                                                                                     aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt                                                  <  1:1275776/139‑1 (MQ=255)
                                                                                     aaTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGt                                                  >  1:154663/1‑139 (MQ=255)
                                                                                      aTGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtc                                                 >  1:1293646/1‑139 (MQ=255)
                                                                                       tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        <  2:1411121/52‑1 (MQ=255)
                                                                                       tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTa                                                                                                                                        >  1:1411121/1‑52 (MQ=255)
                                                                                       tGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                                >  1:391052/1‑139 (MQ=255)
                                                                                          gACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAg                                             <  1:1481297/139‑1 (MQ=255)
                                                                                            ccGCCTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           <  2:1400388/139‑1 (MQ=255)
                                                                                               cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           <  1:665212/136‑1 (MQ=255)
                                                                                               cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCt                                           >  2:665212/1‑136 (MQ=255)
                                                                                               cTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTAATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAACTggg                                        <  1:130356/139‑1 (MQ=255)
                                                                                                 tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            >  2:234049/1‑117 (MQ=255)
                                                                                                 tACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            <  1:234049/117‑1 (MQ=255)
                                                                                                  aCCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcac                                     <  2:391052/139‑1 (MQ=255)
                                                                                                   ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                    >  1:709583/1‑139 (MQ=255)
                                                                                                   ccTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGACGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGcaca                                    <  1:1058299/139‑1 (MQ=255)
                                                                                                      cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATg                                 <  2:1474457/139‑1 (MQ=255)
                                                                                                      cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATg                                 >  1:1907893/1‑139 (MQ=255)
                                                                                                      cACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTAcccccccaga                                                                                                                            >  2:1212974/1‑43 (MQ=255)
                                                                                                       aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                >  1:1386409/1‑139 (MQ=255)
                                                                                                       aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                >  2:1099024/1‑139 (MQ=255)
                                                                                                       aCCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGa                                >  1:911807/1‑139 (MQ=255)
                                                                                                        ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              >  2:1569443/1‑92 (MQ=255)
                                                                                                        ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  2:1386409/139‑1 (MQ=255)
                                                                                                        ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  1:439476/139‑1 (MQ=255)
                                                                                                        ccAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGAt                               <  1:636995/139‑1 (MQ=255)
                                                                                                         cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCACCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              >  2:1934178/1‑91 (MQ=255)
                                                                                                         cAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCACCAATCATTTCCAAAAGCAACAATGATCTCTTTTa                                                                              <  1:1934178/91‑1 (MQ=255)
                                                                                                         cAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                      <  2:815694/35‑1 (MQ=255)
                                                                                                         cAAAACCAGTAAATATTGCTCGGGTAGCAAATTAc                                                                                                                                      >  1:815694/1‑35 (MQ=255)
                                                                                                              ccAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                                <  2:438408/116‑1 (MQ=255)
                                                                                                              ccAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGtct                                                >  1:438408/1‑116 (MQ=255)
                                                                                                               cAGTAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTa                        <  2:147820/139‑1 (MQ=255)
                                                                                                                  tAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       >  1:344726/1‑105 (MQ=255)
                                                                                                                  tAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCa                                                       <  2:344726/105‑1 (MQ=255)
                                                                                                                  tAAATATTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTg                     >  2:1536526/1‑139 (MQ=255)
                                                                                                                      tatTGCTCGGGTAGCAAATTACCCCGCCAGACGAGGTAATTCATCAATCAGTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtag                 <  1:1536526/139‑1 (MQ=255)
                                                                                                                       atTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTaa                                                                                                                     <  2:1399754/38‑1 (MQ=255)
                                                                                                                       atTGCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagt                <  2:1293646/139‑1 (MQ=255)
                                                                                                                          gCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       >  1:1789827/1‑113 (MQ=255)
                                                                                                                          gCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGc                                       <  2:1789827/113‑1 (MQ=255)
                                                                                                                          gCTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAc             >  1:1434418/1‑139 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              >  1:168584/1‑73 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg                                                               >  1:1142159/1‑88 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg                                                               <  2:554696/88‑1 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg                                                               >  1:554696/1‑88 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAgg                                                               <  2:1142159/88‑1 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAt                                                            <  1:143083/91‑1 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGTTAGTAAct            >  2:1476601/1‑138 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            >  2:1980562/1‑139 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  2:188459/139‑1 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            <  2:154663/139‑1 (MQ=255)
                                                                                                                           cTCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAca            >  1:1115078/1‑139 (MQ=255)
                                                                                                                            tCGGGTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAAcat           >  2:1265424/1‑139 (MQ=255)
                                                                                                                                gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              <  1:1435834/68‑1 (MQ=255)
                                                                                                                                gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTa                                                                              >  2:1435834/1‑68 (MQ=255)
                                                                                                                                gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg                    <  2:1384686/126‑1 (MQ=255)
                                                                                                                                gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTgg                    >  1:1384686/1‑126 (MQ=255)
                                                                                                                                gTAGCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGgtagta               >  1:954881/1‑131 (MQ=255)
                                                                                                                                   gCAAATTACCCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTAcc    <  1:1980562/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                     
CTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAG  >  NC_000913/2484753‑2485023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: