Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,709,561 (C)6→7 coding (915/1692 nt) eptB ← KDO phosphoethanolamine transferase, Ca(2+)‑inducible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,709,5551.C98.1% 353.4 / ‑1.1 103coding (921/1692 nt)eptBKDO phosphoethanolamine transferase, Ca(2+)‑inducible
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (53/48);  total (53/50)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 140 total aligned reads displayed.

GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑C‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCC  >  NC_000913/3709418‑3709687
                                                                                                                                          |                                                                                                                                      
gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                       <  2:1711595/139‑1 (MQ=255)
gCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                       >  1:1657669/1‑139 (MQ=255)
   aTGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑ccc                                                                                                                                   >  1:757811/1‑139 (MQ=255)
     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGTGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑ccccc                                                                                                                                 <  1:127523/139‑1 (MQ=255)
     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑ccccc                                                                                                                                 <  2:571118/139‑1 (MQ=255)
     gttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑ccccc                                                                                                                                 <  2:912869/139‑1 (MQ=255)
     gttgtCCGCCATCGTGTTGCTGTAAAACCACATTTCGCTCTGCATAGCGTATAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGCTCTGTTCTTTTACTGTGCGCTGCGGATTATCTTCCGCG‑c‑ccccc                                                                                                                                 >  1:1203528/1‑139 (MQ=255)
      ttgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccc                                                                                                                                >  1:1910236/1‑138 (MQ=255)
       tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccct                                                                                                                               >  1:117951/1‑137 (MQ=255)
       tgtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccct                                                                                                                               <  2:1910236/139‑3 (MQ=255)
        gtCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccctg                                                                                                                              >  2:617358/1‑136 (MQ=255)
           cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACg                                                                                                                           <  1:92206/139‑1 (MQ=255)
           cGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACg                                                                                                                           <  2:117951/139‑1 (MQ=255)
              cATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTAc                                                                                                                        >  1:1385968/1‑139 (MQ=255)
                        cTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCaa                                                                                                              >  1:445610/1‑139 (MQ=255)
                          gTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACg                                                                                                            <  2:445610/139‑1 (MQ=255)
                             gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccctg                                                                                                                              >  2:1930010/1‑115 (MQ=255)
                             gAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccctg                                                                                                                              <  1:1930010/118‑4 (MQ=255)
                              aaCCACATTTCGCTATGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCTAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑C‑CCCCCGTGACGTACAAACATGCGACGCAGt                                                                                                        <  2:1258464/139‑1 (MQ=255)
                                   caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCcgcg                                                                                                                                         <  1:573554/103‑1 (MQ=255)
                                   caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGt                                                                                                        <  2:1298390/134‑1 (MQ=255)
                                   caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGt                                                                                                        >  1:1298390/1‑134 (MQ=255)
                                   caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccct                                                                                                                               <  1:366667/111‑3 (MQ=255)
                                   caTTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccct                                                                                                                               >  2:366667/1‑109 (MQ=255)
                                     tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                       >  2:1001534/1‑102 (MQ=255)
                                     tttCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                       <  1:1001534/102‑1 (MQ=255)
                                         gCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑CTCCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                             <  2:2012249/139‑1 (MQ=255)
                                           tctGCATAGCGTAGAGGTCAGAACTGAATCCTAA‑‑‑CTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                        <  1:836937/139‑1 (MQ=255)
                                               cATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGt                                                                                       <  2:1657669/139‑1 (MQ=255)
                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGt                                                                                       >  1:2040813/1‑131 (MQ=255)
                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccctg                                                                                                                              <  2:1027273/92‑4 (MQ=255)
                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccctg                                                                                                                              >  1:1027273/1‑89 (MQ=255)
                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑ccccc                                                                                                                                 <  2:522094/89‑1 (MQ=255)
                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑ccccc                                                                                                                                 >  2:1786548/1‑89 (MQ=255)
                                                       agagGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑ccccc                                                                                                                                 >  1:522094/1‑89 (MQ=255)
                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACaa                                                                                                                      <  2:1488730/98‑1 (MQ=255)
                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACaa                                                                                                                      >  1:1488730/1‑98 (MQ=255)
                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                        <  1:542184/128‑1 (MQ=255)
                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATca                                                                                   <  1:1193660/133‑1 (MQ=255)
                                                         agGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAg                                                                             >  2:823615/1‑139 (MQ=255)
                                                          ggTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                                                            <  2:221020/139‑1 (MQ=255)
                                                              cgAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAAcc                                                                        >  1:1286158/2‑139 (MQ=255)
                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccc                                                                                                                                >  1:1739994/1‑82 (MQ=255)
                                                              aGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccc                                                                                                                                <  2:1739994/83‑2 (MQ=255)
                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                       <  1:466598/70‑1 (MQ=255)
                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCgcg‑c                                                                                                                                       >  2:466598/1‑70 (MQ=255)
                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTAGAAACAt                                                                                                                  >  2:1092598/1‑90 (MQ=255)
                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACAt                                                                                                                  <  1:1092598/90‑1 (MQ=255)
                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCg                                                                                         <  1:461284/115‑1 (MQ=255)
                                                                     aaTCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAc                                                                 >  1:1086838/1‑139 (MQ=255)
                                                                         cTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcgccg                                                             <  2:757811/139‑1 (MQ=255)
                                                                           aaCTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACgccgcc                                                           <  1:617358/139‑1 (MQ=255)
                                                                                 ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCaa                                                                                                              <  2:1556478/82‑1 (MQ=255)
                                                                                 ttCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCaa                                                                                                              >  1:1556478/1‑82 (MQ=255)
                                                                                  tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcgccgc                                                            <  2:2034900/131‑1 (MQ=255)
                                                                                  tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAAcgccgc                                                            >  1:2034900/1‑131 (MQ=255)
                                                                                  tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa                                                          <  1:595795/133‑1 (MQ=255)
                                                                                  tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGAtttt                                                    >  1:317781/1‑139 (MQ=255)
                                                                                  tCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATCATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGGGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCa                                                          >  2:595795/1‑133 (MQ=255)
                                                                                     gAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTtct                                                 <  2:649030/139‑1 (MQ=255)
                                                                                      aaCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTa                                                                                                                         >  2:771757/1‑66 (MQ=255)
                                                                                      aaCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTa                                                                                                                         <  1:771757/66‑1 (MQ=255)
                                                                                      aaCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccc                                                                                                                                <  2:362782/59‑2 (MQ=255)
                                                                                      aaCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccc                                                                                                                                >  1:362782/1‑58 (MQ=255)
                                                                                      aaCCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑ccccc                                                                                                                                 <  1:1771802/58‑1 (MQ=255)
                                                                                        ccGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCt                                              >  1:1396561/1‑139 (MQ=255)
                                                                                          gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccct                                                                                                                               <  1:1018081/56‑3 (MQ=255)
                                                                                          gcgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑c‑cccccct                                                                                                                               >  2:1018081/1‑54 (MQ=255)
                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTa                                                                                                                         >  1:900566/1‑61 (MQ=255)
                                                                                           cgAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTggg                                           >  2:1166785/1‑139 (MQ=255)
                                                                                            gAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGc                                          <  1:302761/139‑1 (MQ=255)
                                                                                                       gttctTTTAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                               <  1:823615/139‑1 (MQ=255)
                                                                                                           ttttAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTATCCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGt                           <  2:1385968/139‑1 (MQ=255)
                                                                                                             ttAATGTGCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTAt                         >  2:939364/1‑139 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                             <  2:335666/66‑1 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                        >  1:1148894/1‑71 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                        >  1:233022/1‑71 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCgg                                                                                        <  2:233022/71‑1 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg                                  <  2:1841513/125‑1 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCg                                  >  1:1841513/1‑125 (MQ=255)
                                                                                                                  gtgCGCTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCg                               <  1:1962877/128‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTa                                                                                                                         <  1:817975/35‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTa                                                                                                                         >  2:817975/1‑35 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAAc                                                                                                             >  2:1742972/1‑47 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAAc                                                                                                             <  1:1742972/47‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATcac                                                                                  >  2:1989426/1‑74 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGt                                                                            <  2:472541/80‑1 (MQ=255)
                                                                                                                     cgcTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGttt                                    <  2:119349/120‑1 (MQ=255)
                                                                                                                       cTGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGt                                                                                             <  2:572876/61‑1 (MQ=255)
                                                                                                                        tGCGGATTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGc                                                                                          <  1:1471509/63‑1 (MQ=255)
                                                                                                                          cGGATTATCGTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGcc            >  2:1378663/1‑139 (MQ=255)
                                                                                                                             aTTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCaa                                                                                                              <  2:1460211/38‑1 (MQ=255)
                                                                                                                             aTTATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCaa                                                                                                              >  1:1460211/1‑38 (MQ=255)
                                                                                                                               tATCTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGaaaa       <  2:459992/139‑1 (MQ=255)
                                                                                                                                  cTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc    >  1:1860034/1‑139 (MQ=255)
                                                                                                                                  cTTCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAc    >  2:1820732/1‑139 (MQ=255)
                                                                                                                                    tCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCg                                                                                         <  2:887456/52‑1 (MQ=255)
                                                                                                                                    tCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                 <  2:1138565/124‑1 (MQ=255)
                                                                                                                                    tCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCa                 >  1:1138565/1‑124 (MQ=255)
                                                                                                                                    tCCGCGCC‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATAccc  >  2:169009/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                      
GCAATGTTGTCCGCCATCGTGTTGCTGTAGAACCACATTTCGCTCTGCATAGCGTAGAGGTCAGAACTGAATCCTAACTGCTTCAGAACCGCGAAAATGTTCTGTTCTTTTAATGTGCGCTGCGGATTATCTTCCGCG‑C‑CCCCC‑TGACGTACAAACATGCAACGCAGTGAGAGTTTGGTTGCGGTATCACAGGAGTAACCACGGAACGCCGCCAGATTTTTCTCCTGGGCCAGTTTCGGCGTGGTATTACGCTCATAGCCGAAAATACCC  >  NC_000913/3709418‑3709687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑