Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,474,138 (A)6→7 coding (15/594 nt) bdcR → transcriptional repressor for divergent bdcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,474,1321.A98.1% 350.9 / ‑1.5 107coding (9/594 nt)bdcRtranscriptional repressor for divergent bdcA
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base A (58/47);  total (60/47)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 109 total aligned reads displayed.

ACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT  >  NC_000913/4474009‑4474264
                                                                                                                            |                                                                                                                                      
aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        <  2:878602/139‑1 (MQ=255)
aCTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        >  2:1516094/1‑139 (MQ=255)
                       tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        <  1:1702308/116‑1 (MQ=255)
                       tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        >  2:1702308/1‑116 (MQ=255)
                                         ccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        <  2:1105295/98‑1 (MQ=255)
                                         ccATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        >  1:1105295/1‑98 (MQ=255)
                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCGGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                              <  1:783873/130‑1 (MQ=255)
                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCGGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAt                                                                              >  2:783873/1‑130 (MQ=255)
                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              <  2:1816002/98‑1 (MQ=255)
                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              >  1:1816002/1‑98 (MQ=255)
                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:1051831/122‑1 (MQ=255)
                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:1051831/1‑122 (MQ=255)
                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                     >  1:1796804/1‑139 (MQ=255)
                                                   tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGc                                                                     >  2:1548662/1‑139 (MQ=255)
                                                    ccTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCa                                                                    <  1:1516094/139‑1 (MQ=255)
                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              >  2:199170/1‑96 (MQ=255)
                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              <  1:199170/96‑1 (MQ=255)
                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                   >  1:1273502/1‑139 (MQ=255)
                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTa                                                                                                                                       >  2:356843/1‑72 (MQ=255)
                                                     cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTa                                                                                                                                       <  1:356843/72‑1 (MQ=255)
                                                               tatataACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtttt                                                         >  1:1896593/1‑139 (MQ=255)
                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         <  2:46144/71‑1 (MQ=255)
                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGc                                                                                 >  2:558761/1‑111 (MQ=255)
                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGc                                                                                 <  1:558761/111‑1 (MQ=255)
                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     >  2:916226/1‑139 (MQ=255)
                                                                   tAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                     <  1:916226/139‑1 (MQ=255)
                                                                     aCATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCa                                                   >  1:1244805/1‑139 (MQ=255)
                                                                      cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              >  2:1363124/1‑79 (MQ=255)
                                                                      cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              <  1:1363124/79‑1 (MQ=255)
                                                                      cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        <  2:1891926/133‑1 (MQ=255)
                                                                      cATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                        >  1:1891926/1‑133 (MQ=255)
                                                                       aTGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaa                                                 <  2:1896593/139‑1 (MQ=255)
                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         <  1:1891638/66‑1 (MQ=255)
                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         >  2:1891638/1‑66 (MQ=255)
                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTcg                                                                                                                        >  1:2031672/1‑67 (MQ=255)
                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:2005413/1‑101 (MQ=255)
                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:2005413/101‑1 (MQ=255)
                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:276631/1‑139 (MQ=255)
                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  2:1800016/1‑139 (MQ=255)
                                                                        tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaaaa                                                >  1:1231558/1‑139 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAgg                                                                                                               >  2:868923/1‑73 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAgg                                                                                                               <  1:868923/73‑1 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt                                                                                            <  1:515326/92‑1 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAg                                                                                   >  2:157391/1‑101 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAg                                                                                   <  1:157391/101‑1 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAtctc                                                                           >  1:1351385/1‑109 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAAtctc                                                                           <  2:1351385/109‑1 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAgg                                             >  2:930863/1‑139 (MQ=255)
                                                                           aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAGAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCt                                                                                            >  2:515326/1‑92 (MQ=255)
                                                                            ttACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         >  2:1032720/1‑62 (MQ=255)
                                                                            ttACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         <  1:1032720/62‑1 (MQ=255)
                                                                             tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt                                           >  2:1988772/1‑139 (MQ=255)
                                                                               cAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTc                                         >  1:1877974/1‑139 (MQ=255)
                                                                                 aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              <  2:545903/68‑1 (MQ=255)
                                                                                 aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgt                                                                                                              >  1:545903/1‑68 (MQ=255)
                                                                                 aaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGa                                       <  1:1988772/139‑1 (MQ=255)
                                                                                  aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         >  2:1074572/1‑56 (MQ=255)
                                                                                  aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAAtctc                                                                                                                         <  1:1074572/56‑1 (MQ=255)
                                                                                    cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgtcg                                                                                                            >  2:432080/1‑67 (MQ=255)
                                                                                    cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGgtcg                                                                                                            <  1:432080/67‑1 (MQ=255)
                                                                                    cTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGc                                    >  1:193355/1‑139 (MQ=255)
                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:382701/86‑1 (MQ=255)
                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:382701/1‑86 (MQ=255)
                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:744680/1‑86 (MQ=255)
                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                      <  1:1557263/134‑1 (MQ=255)
                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                      >  2:902866/1‑134 (MQ=255)
                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                      >  2:1557263/1‑134 (MQ=255)
                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                      <  1:902866/134‑1 (MQ=255)
                                                                                       ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  2:1851625/1‑139 (MQ=255)
                                                                                         ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                >  2:757672/1‑74 (MQ=255)
                                                                                         ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                <  1:757672/74‑1 (MQ=255)
                                                                                          tAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg                              <  2:1436550/139‑1 (MQ=255)
                                                                                            aCTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgtg                            <  2:193355/139‑1 (MQ=255)
                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:869345/1‑76 (MQ=255)
                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  1:1064027/1‑76 (MQ=255)
                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:1064027/76‑1 (MQ=255)
                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  2:869345/76‑1 (MQ=255)
                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                       >  2:252536/1‑139 (MQ=255)
                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                       <  1:930863/139‑1 (MQ=255)
                                                                                                 cTGTCAACAGGTTTTGTAATGGTCACTAAGAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                       >  2:801782/1‑139 (MQ=255)
                                                                                                  tGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTg                      <  2:1474607/139‑1 (MQ=255)
                                                                                                    tCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGaa                    <  2:1231558/139‑1 (MQ=255)
                                                                                                            ttttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGa            <  2:276631/139‑1 (MQ=255)
                                                                                                             tttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt           <  1:1851625/139‑1 (MQ=255)
                                                                                                              ttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTc                                                       <  1:1266336/94‑1 (MQ=255)
                                                                                                              ttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAtt          >  2:762251/1‑139 (MQ=255)
                                                                                                              ttGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAtt          >  2:1151221/1‑139 (MQ=255)
                                                                                                              atGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCTGCAAAAGTGCTTTTTc                                                       >  2:1266336/2‑94 (MQ=255)
                                                                                                                   aTGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      <  1:348213/58‑1 (MQ=255)
                                                                                                                   aTGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAg                                                                                      >  2:348213/1‑58 (MQ=255)
                                                                                                                     ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCCATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGATTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg   >  2:1936519/1‑139 (MQ=255)
                                                                                                                     ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 <  2:892318/109‑1 (MQ=255)
                                                                                                                     ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  1:976180/1‑109 (MQ=255)
                                                                                                                     ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg   >  1:31838/1‑139 (MQ=255)
                                                                                                                     ggTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTgg   >  1:532837/1‑139 (MQ=255)
                                                                                                                      gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCg                                        <  2:577772/101‑1 (MQ=255)
                                                                                                                      gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCg                                        >  1:577772/1‑101 (MQ=255)
                                                                                                                      gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 >  1:1019043/1‑108 (MQ=255)
                                                                                                                      gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                 <  2:1019043/108‑1 (MQ=255)
                                                                                                                      gTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGt  >  1:501267/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                      
ACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGT  >  NC_000913/4474009‑4474264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: