| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,609,336 | (C)7→8 | intergenic (+13/‑78) | yjjG → / → prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,609,329 | 1 | . | C | 91.8% | 153.5 / 6.2 | 49 | intergenic (+6/‑85) | yjjG/prfC | dUMP phosphatase/peptide chain release factor RF‑3 |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base C (23/22); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
TCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCG > NC_000913/4609193‑4609454 | tcGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc > 2:1092053/1‑139 (MQ=255) tCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgtt > 2:1697599/1‑135 (MQ=255) tCAACGCCGGGCTAGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgtt > 1:1379263/1‑135 (MQ=255) aaTGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGtt > 1:755620/1‑139 (MQ=255) cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgttg < 1:1724404/105‑6 (MQ=255) cacCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgttg > 2:1724404/1‑100 (MQ=255) cgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAATACTGATTGCC‑T‑cc‑ccccc < 1:1142250/92‑1 (MQ=255) cgAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccc > 2:1142250/1‑92 (MQ=255) aaCCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGa > 1:1470317/1‑139 (MQ=255) ccAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATg > 2:1318708/1‑139 (MQ=255) gCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTa > 2:494416/1‑139 (MQ=255) cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCTGCTCCTGTGTAAACACTGATTGCCGC‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAAGTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 1:1582941/139‑1 (MQ=255) cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 1:1092053/139‑1 (MQ=255) cACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 2:1470317/139‑1 (MQ=255) ccTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGaagaaa < 1:1318708/139‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg < 2:1499415/106‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg > 1:1499415/1‑106 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg < 2:874460/106‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggcg > 1:874460/1‑106 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggc < 2:524020/105‑1 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAgcgcggc > 1:524020/1‑105 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgttg > 2:2012394/1‑57 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgttg > 1:941787/1‑57 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgttg < 2:941787/62‑6 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgttg < 1:2012394/62‑6 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccc > 1:797295/1‑57 (MQ=255) tttCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccc < 2:797295/57‑1 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATg > 1:445438/1‑64 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATg < 2:445438/64‑1 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga > 1:1035066/1‑127 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 2:1035066/127‑1 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccc > 2:220981/1‑54 (MQ=255) ttcttcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccc < 1:220981/54‑1 (MQ=255) tcGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTg > 1:1737205/1‑139 (MQ=255) cgctGCACCAAATGGATCAGCTCCTTTGGTATCCCTGGTTGCC‑TCCC‑CCCCCGTTGAAGGGTAAAATATGCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGt < 1:329867/136‑1 (MQ=255) ttGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt < 2:1452394/102‑1 (MQ=255) ttGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgttg > 2:465149/1‑49 (MQ=255) ttGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑cc‑ccccccgttg < 1:465149/54‑6 (MQ=255) ttGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑CCGCCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGAt > 1:1452394/1‑102 (MQ=255) cGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTCGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa < 1:944845/139‑1 (MQ=255) aCTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGTGATGCCGCTTACTCACGAAGAAAGAATTATGACGTTGTCTCCTTAttt > 2:329867/1‑139 (MQ=255) gAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg < 2:644479/121‑1 (MQ=255) gAGCAGCTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg > 1:644479/1‑121 (MQ=255) cTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTg > 2:327083/1‑139 (MQ=255) cTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTg > 1:1799048/1‑139 (MQ=255) cTCCTGTGTAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTg > 1:299201/1‑139 (MQ=255) tgtgTAAACACTGATTGCC‑T‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGaa > 2:489529/1‑139 (MQ=255) tAAACACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAgcg < 1:327083/139‑1 (MQ=255) aaaCACTGATTGCC‑T‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgcc < 1:489529/139‑1 (MQ=255) aaCACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAgaagaa > 2:2022909/1‑93 (MQ=255) aaCACTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAgaagaa < 1:2022909/93‑1 (MQ=255) acacTGATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGcgccg < 2:299201/139‑1 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACt < 2:1534982/78‑1 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACt > 1:1534982/1‑78 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATg < 1:46754/96‑1 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATg > 2:46754/1‑96 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGTCGtt < 1:855639/101‑1 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg < 2:1264460/99‑1 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACg > 1:1264460/1‑99 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGtt > 2:855639/1‑101 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa > 1:848250/1‑110 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTa < 2:848250/110‑1 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTAt < 2:1878256/111‑1 (MQ=255) gATTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTAt > 1:1878256/1‑111 (MQ=255) aTTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga < 1:1658704/85‑1 (MQ=255) aTTGCC‑TCCC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAagaaga > 2:1658704/1‑85 (MQ=255) | TCGGTGGCATCAACGCCGGGCTTGCGACCTGCTGGCTGAATGCACACCATCGCGAGCAACCAGAAGGCATCGCGCCCACCTGGACCGTTTCTTCGTTGCACGAACTGGAGCAGCTCCTGTGTAAACACTGATTGCC‑T‑CC‑CCCCCGTTGATGGGTAAAATAGCCGCAATTTTTCGTTTTCAACAAGCGCGGCGCGATGCCGCTTACTCAAGAAGAAAGAATTATGACGTTGTCTCCTTATTTGCAAGAGGTGGCGAAGCGCCG > NC_000913/4609193‑4609454 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |