Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 1,265,001 1086 bp→GAGG [prfA] [prfA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1265001 1266086 1086 62 [0] [0] 56 [prfA] [prfA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12650000 (0.000)55 (0.700)
+GAGG
38/198 0.7 100% intergenic (+30/‑12) hemA/prfA glutamyl tRNA reductase/peptide chain release factor RF‑1
?NC_000913 1266087 = 0 (0.000)coding (1076/1083 nt) prfA peptide chain release factor RF‑1

TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1264864‑1265000
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTG  >  NC_000913/1266087‑1266223
                                                                                                                                         ||||                                                                                                                                         
TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGA                                                                                                                                             >  1:1006545/1‑139
TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGA                                                                                                                                             >  2:1352214/1‑139
  ACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGG                                                                                                                                           <  2:826377/139‑1
  ACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGG                                                                                                                                           <  2:1006545/139‑1
  ACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGG                                                                                                                                           <  1:1742942/139‑1
                        CAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACAC                                                                                                                     <  2:766108/139‑1
                                      CAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGC                                                                                                       >  2:373097/1‑139
                                            GCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAG                                                                                                 >  1:573516/1‑139
                                            GCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAG                                                                                                 <  2:1150335/139‑1
                                            GCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAG                                                                                                 >  2:253208/1‑139
                                            GCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGAATAATGGAATATCAACAC                                                                                                                     >  2:933049/1‑118
                                            GCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGAATAATGGAATATCAACAC                                                                                                                     <  1:933049/118‑1
                                              CGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACA                                                                                                                      <  1:127180/116‑1
                                              CGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACA                                                                                                                      >  2:127180/1‑116
                                                   GTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTT                                                                                          <  2:1207271/139‑1
                                                          GGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGA                                                                                   >  1:152632/1‑139
                                                          GGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCC                                                                                               >  1:1854907/1‑127
                                                          GGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCC                                                                                               <  2:1854907/127‑1
                                                          GGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGA                                                                                                                                             <  2:1336332/81‑1
                                                          GGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTATTTTTTTACAGGGTGGA                                                                                                                                             >  1:1336332/1‑81
                                                           GATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAA                                                                                                                                      <  1:1852222/87‑1
                                                           GATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAA                                                                                                                                      >  2:1852222/1‑87
                                                                    CGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAA                                                                                                                                   <  1:1892301/81‑1
                                                                    CGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAA                                                                                                                                   >  2:1892301/1‑81
                                                                      CCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCG                                                                        >  2:753795/1‑138
                                                                      CCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCG                                                                        <  1:753795/138‑1
                                                                      CCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAG                                                                                                 >  1:306740/1‑113
                                                                      CCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAG                                                                                                 <  2:306740/113‑1
                                                                            TATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTG                                                                 <  1:52862/139‑1
                                                                             ATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGA                                                                <  1:1352214/139‑1
                                                                                CTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAA                                                                                                                        >  2:1542515/1‑80
                                                                                CTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAA                                                                                                                        <  1:1542515/80‑1
                                                                                 TGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCT                                                            >  1:1610578/1‑139
                                                                                 TGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGG                                                                                                                                >  1:1936964/1‑71
                                                                                 TGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGG                                                                                                                                <  2:1936964/71‑1
                                                                                 TGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGG                                                                                                                                <  2:164412/71‑1
                                                                                 TGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGG                                                                                                                                >  1:164412/1‑71
                                                                                   CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAAT                                                                                                                                  <  2:1242479/67‑1
                                                                                   CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAAT                                                                                                                                  >  1:1242479/1‑67
                                                                                   CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAG                                                                                                                                         >  2:303953/1‑60
                                                                                   CGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAG                                                                                                                                         <  1:303953/60‑1
                                                                                           CCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGC                                                  >  2:1271219/1‑139
                                                                                                 GCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGC                                            <  1:373097/139‑1
                                                                                                 GCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGAGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGC                                            <  1:1271219/139‑1
                                                                                                     GAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGT                                        >  1:1713244/1‑139
                                                                                                     GAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGT                                        >  2:506895/1‑139
                                                                                                     GAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCG                                                                   >  1:239102/1‑112
                                                                                                     GAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCG                                                                   <  2:239102/112‑1
                                                                                                      AGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGAAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGGGAAAGCCCGCGGCTGGTTGCTGAAATCCTGCTGGAGCATGTT                                       >  2:1940402/1‑139
                                                                                                               ACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATa                                                                                                                          <  1:1285532/47‑2
                                                                                                               ACATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATC                                                                                                                          >  2:1285532/1‑47
                                                                                                                CATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCG                                                                   >  2:1994390/1‑101
                                                                                                                CATCATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCG                                                                   <  1:1994390/101‑1
                                                                                                                   CATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGC                          >  1:268940/1‑139
                                                                                                                    ATTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCG                         <  2:152632/139‑1
                                                                                                                     TTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGACAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTA                                      >  2:1420963/1‑125
                                                                                                                     TTTTCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATAAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTA                                      <  1:1420963/125‑1
                                                                                                                        TCTTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCACAGGGCGTACT                     >  1:145418/1‑139
                                                                                                                          taaaaaaagAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGA                                                                >  2:1266168/10‑94
                                                                                                                          taaaaaaagAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGA                                                                <  1:1266168/85‑1
                                                                                                                          TTTTTTTACAGGGTGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTT                   >  1:183169/1‑139
                                                                                                                                       TGGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTT      >  1:1568042/1‑139
                                                                                                                                           GGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTG  <  1:506895/139‑1
                                                                                                                                         ||||                                                                                                                                         
TGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1264864‑1265000
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTTTGGTG  >  NC_000913/1266087‑1266223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.