Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,738,7360GA49.7% 30.9 / 201.2 157A414T (GCT→ACT) malSalpha‑amylase
Reads supporting (aligned to +/- strand):  ref base G (38/41);  new base A (40/38);  total (78/79)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 157 total aligned reads displayed.

ACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTT  >  NC_000913/3738607‑3738867
                                                                                                                                 |                                                                                                                                   
   ggCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGc                                                                                                                         <  2:1043012/139‑1 (MQ=255)
   ggCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTAGAGTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGGTTCTGGTCTGc                                                                                                                         <  1:1146263/139‑1 (MQ=255)
     ctaggATAAATGGTGGGGAAAAAACTGGAGCAGAACGGGTATCGGCTTTTACGATGATCATAGATTCGACAATCTCACGATGTCGCGAGCCTTTTTTCCGGACATCAAAACCGAATCAACTGCCACTTCTGGTCTGCCg                                                                                                                       <  1:1863155/136‑1 (MQ=255)
     cTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATTGGCGATTACGACGATCGTGGATTAGGCGAACTCACTATATCGCTAGCGTTTTTGCCGTATATCAAAACCCAATCAACTGCCGCTTATGGTCTGCCg                                                                                                                       <  1:1888603/139‑1 (MQ=255)
      tGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGCTATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCgg                                                                                                                      >  1:548830/1‑139 (MQ=255)
      tGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGcc                                                                                                                        <  1:269571/137‑1 (MQ=255)
      tGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGcc                                                                                                                        >  2:269571/1‑137 (MQ=255)
              aTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCtt                                                                                                                                  >  2:79597/1‑119 (MQ=255)
              aTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCtat                                                                                                              <  2:1936229/139‑1 (MQ=255)
              aTGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTc                                                                                                                            >  2:1334215/1‑125 (MQ=255)
                  tggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCta                                                                                                               >  2:962108/1‑134 (MQ=255)
                  tggGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCta                                                                                                               <  1:962108/134‑1 (MQ=255)
                    gggAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTg                                                                                                                               <  2:180832/116‑1 (MQ=255)
                      gAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACaa                                                                                                      <  2:1350939/139‑1 (MQ=255)
                        aaaaaCTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCA‑‑‑ACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATgg                                                                                                 <  1:723865/139‑1 (MQ=255)
                            aCTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAAc                                                                                                        >  2:1960843/1‑131 (MQ=255)
                            aCTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAAc                                                                                                        <  1:1960843/131‑1 (MQ=255)
                                gATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATAcc                                                                                            >  1:504921/1‑139 (MQ=255)
                                      aaCGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGcc                                                                                      >  2:619470/1‑139 (MQ=255)
                                       aCGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCa                                                                                     <  2:504921/139‑1 (MQ=255)
                                           atatCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt                                                                                                                   <  2:887535/105‑1 (MQ=255)
                                           atatCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt                                                                                                                   >  1:887535/1‑105 (MQ=255)
                                                ggCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTg                                                                            >  2:1367366/1‑139 (MQ=255)
                                                ggCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTg                                                                            >  2:1109902/1‑139 (MQ=255)
                                                 gCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGa                                                                           <  2:548830/139‑1 (MQ=255)
                                                  cGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGAc                                                                          >  1:1741404/1‑139 (MQ=255)
                                                    aTTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAg                                                                                  <  1:111789/129‑1 (MQ=255)
                                                    aTTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAg                                                                                  >  2:111789/1‑129 (MQ=255)
                                                    aTTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTTTAAAAACAAAATGGATACCCACGCCAAAGCCATTGACgg                                                                        >  1:1484226/1‑139 (MQ=255)
                                                      tACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGa                                                                           >  1:1039983/1‑134 (MQ=255)
                                                              tCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGACTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCAtt                                                                             <  2:1190671/124‑1 (MQ=255)
                                                              tCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGACTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCAtt                                                                             >  1:1190671/1‑124 (MQ=255)
                                                              tCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTc                                                                                                                                 <  1:1959122/72‑1 (MQ=255)
                                                              tCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTc                                                                                                                                 >  2:1959122/1‑72 (MQ=255)
                                                              tCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATAcgccg                                                              >  2:885595/1‑139 (MQ=255)
                                                                cTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCcgcg                                                            <  1:1109902/139‑1 (MQ=255)
                                                                   gATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGa                                                         <  1:133206/139‑1 (MQ=255)
                                                                   gATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGa                                                         >  1:538071/1‑139 (MQ=255)
                                                                        gacgaTCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCg                                                                                                                       <  1:1561478/72‑1 (MQ=255)
                                                                        gacgaTCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCg                                                                                                                       >  2:1561478/1‑72 (MQ=255)
                                                                        gacgaTCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCAtt                                                                             >  1:1836937/1‑114 (MQ=255)
                                                                          cgaTCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTa                                                  <  1:73785/139‑1 (MQ=255)
                                                                           gaTCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTaa                                                 <  2:1446230/139‑1 (MQ=255)
                                                                                      gTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTa                                      >  1:797700/1‑139 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt                                                                                                                   <  1:83879/61‑1 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt                                                                                                                   >  2:83879/1‑61 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGtt                                                                                                                  <  2:1297586/62‑1 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACa                                                                                                       >  2:1597639/1‑73 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACa                                                                                                       <  1:1597639/73‑1 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAAcc                                               <  2:1888494/129‑1 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTaa                                     >  1:1567418/1‑139 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGGTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTaa                                     >  2:1987987/1‑139 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACaa                                                                                                      >  2:189818/1‑74 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTGAACCCa                                             <  2:1449693/131‑1 (MQ=255)
                                                                                       tCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACACCGCGCGATTACTTAACCCa                                             >  1:1449693/1‑131 (MQ=255)
                                                                                        cGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGt                                                                                                                             >  1:71000/1‑50 (MQ=255)
                                                                                        cGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAg                                    >  1:1900890/1‑139 (MQ=255)
                                                                                         gCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCgg                                                                                                                      <  2:1465327/56‑1 (MQ=255)
                                                                                         gCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCgg                                                                                                                      >  1:1465327/1‑56 (MQ=255)
                                                                                         gCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCg                                                                                                                       >  1:1987920/1‑55 (MQ=255)
                                                                                         gCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCg                                                                                                                       <  2:1987920/55‑1 (MQ=255)
                                                                                           tAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGa                                                         <  2:1805318/115‑1 (MQ=255)
                                                                                           tAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGa                                                         >  1:1805318/1‑115 (MQ=255)
                                                                                           tAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCa                                 >  2:258315/1‑139 (MQ=255)
                                                                                             gCCTTTTTGCCGTATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt                                                                                                                   <  2:1342957/55‑1 (MQ=255)
                                                                                             gCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGgtgt                                                                                                                   >  1:1342957/1‑55 (MQ=255)
                                                                                             gCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCtat                                                                                                              <  2:1594261/60‑1 (MQ=255)
                                                                                             gCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCtat                                                                                                              >  1:1594261/1‑60 (MQ=255)
                                                                                             gCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACa                                                                                                       >  2:1424950/1‑67 (MQ=255)
                                                                                             gCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACa                                                                                                       <  1:1424950/67‑1 (MQ=255)
                                                                                             gCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGt                               <  1:250529/139‑1 (MQ=255)
                                                                                             gCCTTTTTGCCGGATATCAAAACCGAATCAACTACCACTTCTGGt                                                                                                                             >  1:1895422/1‑45 (MQ=255)
                                                                                                tttttGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCcgcgc                                                           >  1:1023760/1‑108 (MQ=255)
                                                                                                tttttGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCcgcgc                                                           <  2:1023760/108‑1 (MQ=255)
                                                                                                 ttttGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGc                                                                                                                         >  1:694715/1‑45 (MQ=255)
                                                                                                 ttttGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGc                                                                                                                         <  2:694715/45‑1 (MQ=255)
                                                                                                  tttGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTc                          <  2:1741404/139‑1 (MQ=255)
                                                                                                   ttGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTcc                         >  1:2037550/1‑139 (MQ=255)
                                                                                                   ttGCCGGATATCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCAtt                                                                             <  2:148011/87‑1 (MQ=255)
                                                                                                       cGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGtt                                       >  1:1273623/1‑121 (MQ=255)
                                                                                                       cGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGtt                                       <  2:1273623/121‑1 (MQ=255)
                                                                                                          atatCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATAcc                                                                                            <  2:112795/65‑1 (MQ=255)
                                                                                                          atatCAAAACCGAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATAcc                                                                                            >  1:112795/1‑65 (MQ=255)
                                                                                                           tatCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTAt                 >  1:322954/1‑139 (MQ=255)
                                                                                                                   ccGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGa         <  1:1345792/139‑1 (MQ=255)
                                                                                                                     gAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATg       >  1:1979199/1‑139 (MQ=255)
                                                                                                                     gAATCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATAcc                                                                                            <  1:1491918/54‑1 (MQ=255)
                                                                                                                      aaTCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTa                                      <  1:438518/107‑1 (MQ=255)
                                                                                                                      aaTCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTa                                      >  2:438518/1‑107 (MQ=255)
                                                                                                                      aaTCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCcgc                       <  1:1322409/122‑1 (MQ=255)
                                                                                                                      aaTCAACTACCGCTTCTGGTCTGCCGGTGATCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCcg                        <  2:1229142/121‑1 (MQ=255)
                                                                                                                      aaTCAACTACCGCTTCTGGTCTGCCGGGGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCcg                        >  1:1229142/1‑121 (MQ=255)
                                                                                                                      aaTCAACTACCACTTCTGGTCTGCCGGTGTTCtat                                                                                                              >  1:1650928/1‑35 (MQ=255)
                                                                                                                      aaTCAACTACCACTTCTGGTCTGCCGGTGTTCtat                                                                                                              <  2:1650928/35‑1 (MQ=255)
                                                                                                                      aaTCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCa                                             >  2:515164/1‑100 (MQ=255)
                                                                                                                      aaTCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATgg      >  2:276405/1‑139 (MQ=255)
                                                                                                                      aaTCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATgg      >  1:51356/1‑139 (MQ=255)
                                                                                                                        tCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGtt    >  2:33206/1‑139 (MQ=255)
                                                                                                                        tCAACTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGtt    >  2:1056589/1‑139 (MQ=255)
                                                                                                                          aaCTACCACTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGtttt  <  1:619470/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                   
ACAGGCTGGGATAAATGGTGGGGAAAAAACTGGATCAGAACGGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCGCTAGCCTTTTTGCCGGATATCAAAACCGAATCAACTACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAATGGATACCCACGCCAAAGCCATTGACGGCTATACGCCGCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTCCGCGACTATGGGATTGATGGTTTT  >  NC_000913/3738607‑3738867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑