Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 478,533 C→T A29T (GCC→ACC)  ylaB ← putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913478,5330CT100.0% 313.2 / NA 96A29T (GCC→ACC) ylaBputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/51);  total (45/51)

Only 100 of 103 total aligned reads displayed.

AGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGT  >  NC_000913/478402‑478663
                                                                                                                                   |                                                                                                                                  
aGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGtcagccag                                                                                                                             >  1:1049711/1‑139 (MQ=255)
aGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGtcagccag                                                                                                                             <  2:661815/139‑1 (MQ=255)
aGCGCCTGGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGtcagccag                                                                                                                             >  1:836866/1‑139 (MQ=255)
 gcgcCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGa                                                                                                                            >  1:1554481/1‑139 (MQ=255)
   gcCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATg                                                                                                                          >  1:697835/1‑139 (MQ=255)
          cATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGc                                                                                                                   <  1:1542274/139‑1 (MQ=255)
                 ccAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAgg                                                                                                            <  2:836866/139‑1 (MQ=255)
                   aGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgca                                                                                                          >  2:1127384/1‑139 (MQ=255)
                    gCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         <  1:1913223/139‑1 (MQ=255)
                       ccTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCaa                                                                                                      <  2:697835/139‑1 (MQ=255)
                         tGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATa                                                                                                    <  2:1079225/139‑1 (MQ=255)
                         tGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATa                                                                                                    >  2:114197/1‑139 (MQ=255)
                         tGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATa                                                                                                    <  1:1296457/139‑1 (MQ=255)
                          gCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATAc                                                                                                   >  2:309103/1‑139 (MQ=255)
                                    tttCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATc                                                                                         <  1:595787/139‑1 (MQ=255)
                                       cccTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGCTGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGt                                                                                      <  1:320064/139‑1 (MQ=255)
                                             tgtCGCCATCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGAAGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTccc                                                                                >  1:1167847/1‑139 (MQ=255)
                                             tgtCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGTAATACTGAAAGAATCAGTACTccc                                                                                >  2:1147025/1‑139 (MQ=255)
                                                 gCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGaaa                                                                            <  1:1127384/139‑1 (MQ=255)
                                                  ccACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAAt                                                                           >  2:490797/1‑139 (MQ=255)
                                                          ttGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcc                                                                                                                               <  1:371839/79‑1 (MQ=255)
                                                          ttGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcc                                                                                                                               >  2:371839/1‑79 (MQ=255)
                                                           tGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGAc                                                                  <  1:880869/139‑1 (MQ=255)
                                                              cTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                               <  1:490797/139‑1 (MQ=255)
                                                              cTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                               <  2:1555375/139‑1 (MQ=255)
                                                               tCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCaa                                                                                                      >  1:113895/1‑99 (MQ=255)
                                                               tCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCaa                                                                                                      <  2:113895/99‑1 (MQ=255)
                                                                  aaTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGt                                                           >  1:1078251/1‑139 (MQ=255)
                                                                        gACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGtt                                                     <  2:1554481/139‑1 (MQ=255)
                                                                         aCGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCt                                                                                                                        >  1:1593831/1‑71 (MQ=255)
                                                                         aCGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCt                                                                                                                        <  2:1593831/71‑1 (MQ=255)
                                                                           gcgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTtctc                                                  <  2:1049711/139‑1 (MQ=255)
                                                                            cgGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACGGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                                 >  1:142163/1‑139 (MQ=255)
                                                                             gggAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACGGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCAc                                                <  2:142163/139‑1 (MQ=255)
                                                                                        gTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCt                                     >  2:1159917/1‑139 (MQ=255)
                                                                                         tATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCtt                                    >  2:1287851/1‑139 (MQ=255)
                                                                                           tCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGAAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCAACAGCATATCCttat                                  >  2:971589/1‑139 (MQ=255)
                                                                                            ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAGTACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGAtctc                                                  >  1:1200601/1‑122 (MQ=255)
                                                                                            ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTc                                                          >  1:149587/1‑114 (MQ=255)
                                                                                            ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTc                                                          <  2:149587/114‑1 (MQ=255)
                                                                                            ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTtctc                                                  <  2:1200601/122‑1 (MQ=255)
                                                                                            ccAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCttatt                                 >  2:1902762/1‑139 (MQ=255)
                                                                                             cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcca                                                                                                                              >  2:1051214/1‑45 (MQ=255)
                                                                                             cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgtcagcca                                                                                                                              <  1:1051214/45‑1 (MQ=255)
                                                                                             cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         <  2:1612259/66‑1 (MQ=255)
                                                                                             cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         >  1:1612259/1‑66 (MQ=255)
                                                                                             cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTc                                                          >  2:2055104/1‑113 (MQ=255)
                                                                                             cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCt                                     >  1:890001/1‑134 (MQ=255)
                                                                                             cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCt                                     <  2:890001/134‑1 (MQ=255)
                                                                                              aGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAAt                                                                                          <  2:261137/80‑1 (MQ=255)
                                                                                              aGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAAt                                                                                          >  1:261137/1‑80 (MQ=255)
                                                                                               gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGtt                                                     >  2:294681/1‑116 (MQ=255)
                                                                                               gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGtt                                                     <  1:294681/116‑1 (MQ=255)
                                                                                                ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:1159917/139‑1 (MQ=255)
                                                                                                ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:114197/139‑1 (MQ=255)
                                                                                                ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:1101491/139‑1 (MQ=255)
                                                                                                ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  2:1019846/139‑1 (MQ=255)
                                                                                                ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:1902762/139‑1 (MQ=255)
                                                                                                ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:1183096/139‑1 (MQ=255)
                                                                                                ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:719219/139‑1 (MQ=255)
                                                                                                ctctTCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCATTATTAaca                             >  1:117997/1‑139 (MQ=255)
                                                                                                   ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         >  1:730677/1‑60 (MQ=255)
                                                                                                   ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         <  2:730677/60‑1 (MQ=255)
                                                                                                   ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCata                                         >  2:1739920/1‑124 (MQ=255)
                                                                                                   ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCata                                         <  1:1739920/124‑1 (MQ=255)
                                                                                                   ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAg                          >  1:1817863/1‑139 (MQ=255)
                                                                                                   ttCAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAg                          <  1:309103/139‑1 (MQ=255)
                                                                                                     cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         >  1:1232498/1‑58 (MQ=255)
                                                                                                     cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGgcag                                                                                                         <  2:1232498/58‑1 (MQ=255)
                                                                                                     cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             >  1:853535/1‑134 (MQ=255)
                                                                                                     cAATAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  2:853535/134‑1 (MQ=255)
                                                                                                      aaTAAACGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAgtgt                       <  1:813312/139‑1 (MQ=255)
                                                                                                           aCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                                 >  2:1991461/1‑76 (MQ=255)
                                                                                                           aCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTcc                                                                                 <  1:1991461/76‑1 (MQ=255)
                                                                                                           aCGATGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                  <  2:117997/139‑1 (MQ=255)
                                                                                                               tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                               >  2:1160901/1‑90 (MQ=255)
                                                                                                               tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAg                                                               <  1:1160901/90‑1 (MQ=255)
                                                                                                               tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                                 >  2:348440/1‑104 (MQ=255)
                                                                                                               tGTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCa                                                 <  1:348440/104‑1 (MQ=255)
                                                                                                                gTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       <  2:1899769/113‑1 (MQ=255)
                                                                                                                gTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       >  1:1899769/1‑113 (MQ=255)
                                                                                                                gTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             >  2:167881/1‑123 (MQ=255)
                                                                                                                gTTTCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAaca                             <  1:167881/123‑1 (MQ=255)
                                                                                                                   tCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGtt                                                     >  2:1128539/1‑96 (MQ=255)
                                                                                                                   tCTACCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGtt                                                     <  1:1128539/96‑1 (MQ=255)
                                                                                                                      aCCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                  >  1:511048/1‑128 (MQ=255)
                                                                                                                      aCCTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                  <  2:511048/128‑1 (MQ=255)
                                                                                                                       ccTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       <  1:1030650/106‑1 (MQ=255)
                                                                                                                       ccTGCTGATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATc                                       >  2:1030650/1‑106 (MQ=255)
                                                                                                                         tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                  >  1:408779/1‑125 (MQ=255)
                                                                                                                         tgctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGa                  <  2:408779/125‑1 (MQ=255)
                                                                                                                          gctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATtg   >  2:1777295/1‑139 (MQ=255)
                                                                                                                           ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGa                                                                                                <  1:998112/45‑1 (MQ=255)
                                                                                                                           ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                     >  1:1333991/1‑56 (MQ=255)
                                                                                                                           ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                     <  2:1333991/56‑1 (MQ=255)
                                                                                                                           ctgATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATtgt  >  1:1027707/1‑139 (MQ=255)
                                                                                                                             gATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                     >  1:111696/1‑54 (MQ=255)
                                                                                                                             gATGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTa                                                                                     <  2:111696/54‑1 (MQ=255)
                                                                                                                              aTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCaa                                                               <  1:1282002/75‑2 (MQ=255)
                                                                                                                              aTGGGTCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGACGACCAg                                                               >  2:1282002/1‑75 (MQ=255)
                                                                                                                                   |                                                                                                                                  
AGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGT  >  NC_000913/478402‑478663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑