Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	480,334	C→T	*125* (TAG→TAA)	tomB ←	Hha toxicity attenuator, conjugation‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	480,334	0	C	T	100.0%	272.5 / NA	83	*125* (TAG→TAA)	tomB	Hha toxicity attenuator, conjugation‑related protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/41);  total (42/41)

GATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGT  >  NC_000913/480202‑480464                                                                                                                                     |                                                                                                                                   gATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACaa                                                                                                                              >  2:1067656/1‑139 (MQ=255)   tAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGa                                                                                                                            <  2:299602/139‑1 (MQ=255)      ttCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATaa                                                                                                                         <  2:1013211/139‑1 (MQ=255)                                gCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                 <  2:670736/105‑1 (MQ=255)                                gCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTaa                                                                                                                                 >  1:670736/1‑105 (MQ=255)                                  tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATa                                                                                                                          >  1:1753627/1‑110 (MQ=255)                                  tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATa                                                                                                                          <  2:1753627/110‑1 (MQ=255)                                  tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                 >  2:771013/1‑135 (MQ=255)                                  tCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc                                                                                                 <  1:771013/135‑1 (MQ=255)                                   ccAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGa                                                                                            >  1:1976528/1‑139 (MQ=255)                                          gtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaaca                                                                                     >  1:1695674/1‑139 (MQ=255)                                                                      aTTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGc                                                         >  2:1010328/1‑139 (MQ=255)                                                                      aTTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAAACGAATAGTCGATTACCTGACTTCCGc                                                         >  2:2008146/1‑139 (MQ=255)                                                                      aTTAAATAATCGGTTTTCGTGAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCGGACTTCCGc                                                         >  1:1210822/1‑139 (MQ=255)                                                                       ttAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                         <  2:246894/106‑1 (MQ=255)                                                                       ttAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                         >  1:246894/1‑106 (MQ=255)                                                                       ttAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGcc                                                        >  2:1625805/1‑139 (MQ=255)                                                                       ttAAATAATCGGCTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGcc                                                        >  1:532007/1‑139 (MQ=255)                                                                              atCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCa                                                       >  1:1663427/1‑133 (MQ=255)                                                                              atCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCa                                                       <  2:1663427/133‑1 (MQ=255)                                                                              atCGGCTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTg                                                 <  2:532007/139‑1 (MQ=255)                                                                               tCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTc                                                                          <  2:1286170/113‑1 (MQ=255)                                                                               acGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTc                                                                          >  1:1286170/2‑113 (MQ=255)                                                                                cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAGGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGAt                                                                       <  1:1510510/115‑1 (MQ=255)                                                                                cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAGGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGAt                                                                       >  2:1510510/1‑115 (MQ=255)                                                                                cGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGaa                                               >  2:318928/1‑139 (MQ=255)                                                                                  gTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGa                                             <  2:1695674/139‑1 (MQ=255)                                                                                  gTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGa                                             <  2:570751/139‑1 (MQ=255)                                                                                    tttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCa                                                       >  1:2004653/1‑127 (MQ=255)                                                                                    tttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCa                                                       <  2:2004653/127‑1 (MQ=255)                                                                                     ttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGa                                                                                                               >  1:329891/1‑70 (MQ=255)                                                                                     ttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGa                                                                                                               <  2:329891/70‑1 (MQ=255)                                                                                     ttCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATcc                                          <  2:1588092/139‑1 (MQ=255)                                                                                         ttAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCa                                      <  1:318928/139‑1 (MQ=255)                                                                                           aaaGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGGCAAAACAACGGaa                                                                               >  1:621634/1‑96 (MQ=255)                                                                                           aaaGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGGCAAAACAACGGaa                                                                               <  2:621634/96‑1 (MQ=255)                                                                                             aGGTTTTTCGGACATACTTCTTCCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                  <  2:1210822/139‑1 (MQ=255)                                                                                             aGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                               >  2:1746808/1‑94 (MQ=255)                                                                                             aGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                               <  1:1746808/94‑1 (MQ=255)                                                                                             aGGTTTGTCTGACATACTTCGACCTATGTGTGTAATAAGTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGAGTCCCTGACTTGCGCCATTTCTGAAGATCCTGCATAtt                                  <  1:2008146/139‑1 (MQ=255)                                                                                                 ttttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                               >  2:1154959/1‑90 (MQ=255)                                                                                                 ttttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGaa                                                                               <  1:1154959/90‑1 (MQ=255)                                                                                                  tttCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATAc                             >  1:1246947/1‑139 (MQ=255)                                                                                                          aTACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        >  1:1625031/1‑120 (MQ=255)                                                                                                          aTACTTCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        <  2:1625031/120‑1 (MQ=255)                                                                                                               tCTACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                <  2:1976528/139‑1 (MQ=255)                                                                                                                 tACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                <  2:1301980/137‑1 (MQ=255)                                                                                                                 tACCTATGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGGCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                >  1:1301980/1‑137 (MQ=255)                                                                                                                      aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGa                                                                                                               <  1:1738801/37‑1 (MQ=255)                                                                                                                      aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGa                                                                                                               >  2:1738801/1‑37 (MQ=255)                                                                                                                      aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATc                                           >  2:939724/1‑105 (MQ=255)                                                                                                                      aTGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATc                                           <  1:939724/105‑1 (MQ=255)                                                                                                                       tGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                         >  1:1312007/1‑58 (MQ=255)                                                                                                                       tGGTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaa                                                                                         <  2:1312007/58‑1 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTGGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                >  2:1274046/1‑130 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACCAACCAACGGAATAGTCGATTACCTGCCTTCCGc                                                         <  1:515191/89‑1 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaacaa                                                                                    >  2:1093179/1‑62 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAaacaa                                                                                    <  1:1093179/62‑1 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAAt                                                                              >  2:715598/1‑68 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAAt                                                                              <  1:715598/68‑1 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGc                                                         >  2:515191/1‑89 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGa                                                >  1:350688/1‑98 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGa                                                <  2:350688/98‑1 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                          <  2:605068/120‑1 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt                          >  1:605068/1‑120 (MQ=255)                                                                                                                        ggTTGTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                <  1:1274046/130‑1 (MQ=255)                                                                                                                            gTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                  <  1:842033/108‑1 (MQ=255)                                                                                                                            gTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                  >  2:842033/1‑108 (MQ=255)                                                                                                                            gTAATAATTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtg   <  1:1625805/139‑1 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACACCGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtgt  >  1:1517961/1‑139 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGaa                                               >  1:1305920/1‑94 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGaa                                               <  2:1305920/94‑1 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAg                                              <  2:199598/95‑1 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAg                                              >  1:199598/1‑95 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        >  1:1445765/1‑101 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTg                                        <  2:1445765/101‑1 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                >  2:559636/1‑125 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                >  1:640123/1‑125 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                <  2:640123/125‑1 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGaa                <  1:559636/125‑1 (MQ=255)                                                                                                                             taataaTTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtgt  >  1:1711727/1‑139 (MQ=255)                                                                                                                               ataatTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                  >  1:1652378/1‑105 (MQ=255)                                                                                                                               ataatTTTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATAtt                                  <  2:1652378/105‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   GATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGT  >  NC_000913/480202‑480464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑