Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	494,419	G→A	P5P (CCG→CCA)	recR →	gap repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	494,419	0	G	A	100.0%	138.5 / NA	45	P5P (CCG→CCA)	recR	gap repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/20);  total (25/20)

CTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGC  >  NC_000913/494289‑494553                                                                                                                                   |                                                                                                                                       cTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAac                                                                                                                                <  1:1061996/139‑1 (MQ=255)                                  cgtATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCggg                                                                                               >  2:1111400/1‑139 (MQ=255)                                       tGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAaca                                                                                                                               >  1:1671537/1‑102 (MQ=255)                                       tGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAaca                                                                                                                               <  2:1671537/102‑1 (MQ=255)                                       tGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTg                                                                                          >  1:1329335/1‑139 (MQ=255)                                              aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGaa                                                                                   >  1:390984/1‑139 (MQ=255)                                                gCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt                                                                                 <  1:1149780/139‑1 (MQ=255)                                                    aaagaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgc                                                                             <  2:660967/139‑1 (MQ=255)                                                       gaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCa                                                                          <  2:390984/139‑1 (MQ=255)                                                              tGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATg                                                                   >  1:654311/1‑139 (MQ=255)                                                                   tctGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACGGGCCCACTGTTAACTCAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGtt                                                              <  1:802942/139‑1 (MQ=255)                                                                           ctccGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgctgc                                                      <  1:1111400/139‑1 (MQ=255)                                                                            tccGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgctgct                                                     >  1:75164/1‑139 (MQ=255)                                                                               ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgcg                                                                            <  1:1762461/113‑1 (MQ=255)                                                                               ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgcg                                                                            >  2:1762461/1‑113 (MQ=255)                                                                               ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgc                                                         <  2:1951936/132‑1 (MQ=255)                                                                               ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgc                                                         >  1:1951936/1‑132 (MQ=255)                                                                               ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                          <  2:949842/131‑1 (MQ=255)                                                                               ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                          >  1:949842/1‑131 (MQ=255)                                                                               ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCa                                                  >  2:180288/1‑139 (MQ=255)                                                                                 aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt                                                                                 <  1:876319/106‑1 (MQ=255)                                                                                 aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt                                                                                 >  2:876319/1‑106 (MQ=255)                                                                                 aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                          >  2:1980648/1‑129 (MQ=255)                                                                                 aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                          <  1:1980648/129‑1 (MQ=255)                                                                                 aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAgc                                                >  2:665219/1‑139 (MQ=255)                                                                                 aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAgc                                                >  2:1687669/1‑139 (MQ=255)                                                                                   tGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCt                                                                                                                          <  1:808562/63‑1 (MQ=255)                                                                                   tGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCt                                                                                                                          >  2:808562/1‑63 (MQ=255)                                                                                   tGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAgcgc                                              >  2:330572/1‑139 (MQ=255)                                                                                                  gCTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCggg                               <  2:75164/139‑1 (MQ=255)                                                                                                   cTTTAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGa                              <  1:180288/139‑1 (MQ=255)                                                                                                    tttAAGATGCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGAt                             <  1:330572/139‑1 (MQ=255)                                                                                                       aaGATGCCGTTCTGATGAAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCAGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCg                          >  2:1286515/1‑139 (MQ=255)                                                                                                            gCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTgg                     >  1:775314/1‑139 (MQ=255)                                                                                                            gCCGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTgg                     >  2:769617/1‑139 (MQ=255)                                                                                                             ccGTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGgc                    >  2:1009939/1‑139 (MQ=255)                                                                                                               gTTCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGgcgc                  <  2:1329335/139‑1 (MQ=255)                                                                                                                ttCTGATGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCa                 >  2:1979786/1‑139 (MQ=255)                                                                                                                      tGCAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCt           <  1:1989299/139‑1 (MQ=255)                                                                                                                        cAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACt                                                                                                              <  1:1165869/38‑1 (MQ=255)                                                                                                                        cAAACCAGCCCACTGTTAACACAGCTTATGGAAGCACt                                                                                                              >  2:1165869/1‑38 (MQ=255)                                                                                                                          aaCCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgc                                                                             >  2:1939257/1‑69 (MQ=255)                                                                                                                          aaCCAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgc                                                                             <  1:1939257/69‑1 (MQ=255)                                                                                                                             cAGCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCgg    >  2:703751/1‑139 (MQ=255)                                                                                                                               gCCCACTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGc  >  2:925702/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                       CTGGTGGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGC  >  NC_000913/494289‑494553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑