Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	47,776	G→A	*177* (TAG→TAA)  S3N (AGC→AAC)	kefF → kefC →	potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	47,776	0	G	A	100.0%	222.4 / NA	69	*177* (TAG→TAA)  S3N (AGC→AAC)	kefF kefC	potassium‑efflux system ancillary protein for KefC, glutathione‑regulated, quinone oxidoreductase, FMN‑dependent potassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/33);  total (36/33)

AATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGG  >  NC_000913/47641‑47900                                                                                                                                        |                                                                                                                             aaTCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCa                                                                                                                           <  1:454003/139‑1 (MQ=255)             gCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCa                                                                                                               >  2:848603/1‑139 (MQ=255)                           cACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTAtc                                                                                                 >  2:527194/1‑139 (MQ=255)                                tttGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                           <  2:1358350/124‑1 (MQ=255)                                tttGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGGCAGTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATTACCATACGCTGATTCAGgcg                                                                                                           >  1:1358350/1‑124 (MQ=255)                                         cactgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                           >  2:1902134/1‑115 (MQ=255)                                         cactgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                           <  1:1902134/115‑1 (MQ=255)                                          actgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCTACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc                                                                                  <  1:175099/139‑1 (MQ=255)                                            tgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgc                                                                                    <  1:1203276/135‑1 (MQ=255)                                            tgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgc                                                                                    >  2:1203276/1‑135 (MQ=255)                                              cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAAcc                                                                                                                            >  1:968741/1‑93 (MQ=255)                                                   ttATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAc                                                                         >  1:784400/1‑139 (MQ=255)                                                   ttATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAc                                                                         >  2:1898010/1‑139 (MQ=255)                                                    tATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                                        <  1:527194/139‑1 (MQ=255)                                                        tgtgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCAtt                                                                    >  1:1864775/1‑139 (MQ=255)                                                         gtgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTg                                                                   <  2:784400/139‑1 (MQ=255)                                                             cgacgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGt                                                               <  1:436685/139‑1 (MQ=255)                                                                cgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcg                                                                                   >  1:1723051/1‑116 (MQ=255)                                                                cgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcg                                                                                   <  2:1723051/116‑1 (MQ=255)                                                                        tCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTc                                                    <  1:848603/139‑1 (MQ=255)                                                                                cAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAAcc                                                                                                                            <  1:155771/59‑1 (MQ=255)                                                                                cAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAt                                                                              >  2:1047322/1‑105 (MQ=255)                                                                                cAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAt                                                                              <  1:1047322/105‑1 (MQ=255)                                                                                cAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                         >  2:182621/1‑126 (MQ=255)                                                                                cAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                         <  1:182621/126‑1 (MQ=255)                                                                                  ggCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGt                                                               >  2:1113308/1‑118 (MQ=255)                                                                                  ggCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGt                                                               <  1:1113308/118‑1 (MQ=255)                                                                                     gcgTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                         >  2:146064/1‑121 (MQ=255)                                                                                     gcgTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                         <  1:146064/121‑1 (MQ=255)                                                                                        tCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTc                                                                                                                >  1:616682/1‑63 (MQ=255)                                                                                        tCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTc                                                                                                                <  2:616682/63‑1 (MQ=255)                                                                                        tCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTgg                                    >  2:936129/1‑139 (MQ=255)                                                                                              taAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc                                                                                  <  1:762358/87‑1 (MQ=255)                                                                                              taAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc                                                                                  >  2:762358/1‑87 (MQ=255)                                                                                                   aaCGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCg                         >  1:846467/1‑139 (MQ=255)                                                                                                    aCGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGc                        <  2:1864775/139‑1 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                           <  2:1960825/55‑1 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                           >  1:1960825/1‑55 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCgg                                                                                        >  2:1110347/1‑74 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCgg                                                                                        <  1:1110347/74‑1 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCAtt                                                                    >  2:1836946/1‑94 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCAtt                                                                    <  1:1836946/94‑1 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt                                              <  2:1835919/116‑1 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt                                              >  1:1835919/1‑116 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc                       >  1:1130253/1‑139 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATAGcc                       >  2:1326411/1‑139 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCATGGTCTGGGATCGGTACTTGGATACCTGATCGcc                       >  2:1320083/1‑139 (MQ=255)                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTGCGGCAGCGCTGATTGTACCCATTCCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc                       >  1:1517152/1‑139 (MQ=255)                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCa                                                                                                                           >  1:1084991/1‑38 (MQ=255)                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCa                                                                                                                           <  2:1084991/38‑1 (MQ=255)                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGTTTTATCTCGGTTCGGCAGCGCTGa                                                                               <  1:1036074/82‑1 (MQ=255)                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCCCTGa                                                                               >  2:1036074/1‑82 (MQ=255)                                                                                                           ctgGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgc                                                                                                            <  1:58956/48‑1 (MQ=255)                                                                                                           ctgGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGgc                                                                                                            >  2:58956/1‑48 (MQ=255)                                                                                                                   gCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt                                              >  2:1193792/1‑102 (MQ=255)                                                                                                                   gCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt                                              <  1:1193792/102‑1 (MQ=255)                                                                                                                         ggCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                           <  2:180751/35‑1 (MQ=255)                                                                                                                         ggCCCATCATGGATAACCATACGCTGATTCAGgcg                                                                                                           >  1:180751/1‑35 (MQ=255)                                                                                                                         ggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTg   <  2:846467/139‑1 (MQ=255)                                                                                                                         ggCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTg   <  1:1898010/139‑1 (MQ=255)                                                                                                                          gCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc                                                                                  >  2:352747/1‑59 (MQ=255)                                                                                                                          gCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc                                                                                  <  1:352747/59‑1 (MQ=255)                                                                                                                          gCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgg  >  1:134971/1‑139 (MQ=255)                                                                                                                            ccATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGt                                                     >  1:860090/1‑86 (MQ=255)                                                                                                                            ccATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGt                                                     <  2:860090/86‑1 (MQ=255)                                                                                                                             catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAt                                                                              <  1:978607/60‑1 (MQ=255)                                                                                                                             catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAt                                                                              >  2:978607/1‑60 (MQ=255)                                                                                                                             catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                     >  2:330459/1‑117 (MQ=255)                                                                                                                             catcatGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                     <  1:330459/117‑1 (MQ=255)                                                                                                                                        |                                                                                                                             AATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGG  >  NC_000913/47641‑47900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑