Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	50,044	G→A	W74* (TGG→TGA)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	50,044	0	G	A	100.0%	275.9 / NA	84	W74* (TGG→TGA)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/42);  total (42/42)

GGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAG  >  NC_000913/49911‑50177                                                                                                                                      |                                                                                                                                      ggTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGaa                                                                                                                                  <  2:1959279/139‑1 (MQ=255)                         aaCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCgcggc                                                                                                          <  1:1932562/139‑1 (MQ=255)                          aCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            >  2:1648832/1‑136 (MQ=255)                          aCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            <  1:1648832/136‑1 (MQ=255)                          aCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCgcggcg                                                                                                         >  1:1489059/1‑139 (MQ=255)                          aCCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCgcggcg                                                                                                         <  2:1489059/139‑1 (MQ=255)                           cccGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGAt                                                                                                                        >  2:1592948/1‑123 (MQ=255)                           cccGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGAt                                                                                                                        <  1:1592948/123‑1 (MQ=255)                                   tATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGAc                                                                                                <  1:2009344/139‑1 (MQ=255)                                        gccgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTAcc                                                                                           <  1:262342/139‑1 (MQ=255)                                         ccgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACg                                                                                               <  2:537286/134‑1 (MQ=255)                                         ccgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACg                                                                                               >  1:537286/1‑134 (MQ=255)                                          cgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTgtgg                                                                                                    >  2:386637/1‑128 (MQ=255)                                          cgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTgtgg                                                                                                    <  1:386637/128‑1 (MQ=255)                                          cgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAg                                                                                         >  2:660402/1‑139 (MQ=255)                                          cgccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAg                                                                                         >  1:520855/1‑139 (MQ=255)                                            ccATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGaa                                                                                       >  1:549454/1‑139 (MQ=255)                                                    gggAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGAt                                                                                                                        >  1:723761/1‑98 (MQ=255)                                                    gggAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGAt                                                                                                                        <  2:723761/98‑1 (MQ=255)                                                       aatcaatcGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGAt                                                                            >  1:614736/1‑139 (MQ=255)                                                           aatcGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTg                                                                                                                                    >  1:1540914/1‑79 (MQ=255)                                                           aatcGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTg                                                                                                                                    <  2:1540914/79‑1 (MQ=255)                                                           aatcGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGaaa                                                                                      <  1:1569580/125‑1 (MQ=255)                                                           aatcGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGaaa                                                                                      >  2:1569580/1‑125 (MQ=255)                                                                gtcgtcCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGcggcgg                                                                   >  2:584692/1‑139 (MQ=255)                                                                 tcgtcCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            <  2:1712537/97‑1 (MQ=255)                                                                 tcgtcCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            >  1:1712537/1‑97 (MQ=255)                                                                    tcCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcgc                                                               >  2:374610/1‑139 (MQ=255)                                                                     ccGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcgcg                                                              <  2:614736/139‑1 (MQ=255)                                                                      cGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCgtg                                                                                                       >  1:999834/1‑97 (MQ=255)                                                                      cGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCgtg                                                                                                       <  2:999834/97‑1 (MQ=255)                                                                             aGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGaa                                                      <  1:660402/139‑1 (MQ=255)                                                                             aGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGATTATGaa                                                      <  1:1348720/139‑1 (MQ=255)                                                                               gACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACa                                                    >  2:943691/1‑139 (MQ=255)                                                                               gACGCAAAAATATTATCCTCAGCAGTCAACCGGATACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            <  2:685914/83‑1 (MQ=255)                                                                               gACGCAAAAATATTATCCTCAGCAGTCAACCGGATACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            >  1:685914/1‑83 (MQ=255)                                                                                aCGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATc                                                                                    >  1:2029932/1‑106 (MQ=255)                                                                                aCGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATc                                                                                    <  2:2029932/106‑1 (MQ=255)                                                                                aCGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAg                                                   <  1:374610/139‑1 (MQ=255)                                                                                     aaaaTATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTAcc                                                                                           >  1:2122/1‑94 (MQ=255)                                                                                     aaaaTATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTAcc                                                                                           <  2:2122/94‑1 (MQ=255)                                                                                           ttatCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCgcggcg                                                                                                         <  2:1513500/74‑1 (MQ=255)                                                                                           ttatCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCgcggcg                                                                                                         >  1:1513500/1‑74 (MQ=255)                                                                                            tatCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            <  1:1703247/70‑1 (MQ=255)                                                                                            tatCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            >  2:1703247/1‑70 (MQ=255)                                                                                              tCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcgcg                                                              >  1:51546/1‑114 (MQ=255)                                                                                              tCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcgcg                                                              <  2:51546/114‑1 (MQ=255)                                                                                              tCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAgc                                     >  2:1173843/1‑139 (MQ=255)                                                                                                  cagcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTgtggtg                                                                                                  >  2:2040721/1‑74 (MQ=255)                                                                                                  cagcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTgtggtg                                                                                                  <  1:2040721/74‑1 (MQ=255)                                                                                                   agcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGcggcgg                                                                   >  2:1316662/1‑104 (MQ=255)                                                                                                   agcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGcggcgg                                                                   <  1:1316662/104‑1 (MQ=255)                                                                                                   agcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcgcg                                                              <  2:97057/109‑1 (MQ=255)                                                                                                   agcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                                <  1:1173843/139‑1 (MQ=255)                                                                                                   agcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCaaa                                >  1:130619/1‑139 (MQ=255)                                                                                                   agcagTCAACCGGGTACGGACGATCGCGCAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcgcg                                                              >  1:97057/1‑109 (MQ=255)                                                                                                    gcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGttt                                                           <  1:434947/111‑1 (MQ=255)                                                                                                    gcagTCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGttt                                                           >  2:434947/1‑111 (MQ=255)                                                                                                        tCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcg                                                                <  2:69322/102‑1 (MQ=255)                                                                                                        tCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcg                                                                >  1:69322/1‑102 (MQ=255)                                                                                                        tCAACCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTg                           >  1:812905/1‑139 (MQ=255)                                                                                                           aCCGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTAt                        <  1:584692/139‑1 (MQ=255)                                                                                                            ccGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATc                       <  1:943691/139‑1 (MQ=255)                                                                                                             cGGGTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCt                      >  1:788199/1‑139 (MQ=255)                                                                                                               ggTACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGa                    >  1:537990/1‑139 (MQ=255)                                                                                                                 tACGGACGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACg                  >  1:345366/1‑139 (MQ=255)                                                                                                                      aCGATCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCatat             <  2:537990/139‑1 (MQ=255)                                                                                                                         aTCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCa                                                                                          >  1:1235564/1‑59 (MQ=255)                                                                                                                         aTCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCa                                                                                          <  2:1235564/59‑1 (MQ=255)                                                                                                                         aTCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATTGTGATTGGCGGCGGt                                                                  <  2:2020811/83‑1 (MQ=255)                                                                                                                         aTCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGt                                                                  >  1:2020811/1‑83 (MQ=255)                                                                                                                          tCGCGTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGAc         >  2:744847/1‑139 (MQ=255)                                                                                                                           cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            <  1:183409/39‑1 (MQ=255)                                                                                                                           cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATcgcg                                                                                                            >  2:183409/1‑39 (MQ=38)                                                                                                                           cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                                  >  1:897435/1‑113 (MQ=255)                                                                                                                           cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCa                                  <  2:897435/113‑1 (MQ=255)                                                                                                                           cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACg        <  1:744847/139‑1 (MQ=255)                                                                                                                           cgcgTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACg        <  2:788199/139‑1 (MQ=255)                                                                                                                              gTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCatat             >  1:983534/1‑131 (MQ=255)                                                                                                                              gTAACGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCatat             <  2:983534/131‑1 (MQ=255)                                                                                                                                aaCGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGaa   >  2:680417/1‑139 (MQ=255)                                                                                                                                 aCGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAg  <  2:520855/139‑1 (MQ=255)                                                                                                                                  cGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcg                                                                >  1:825097/1‑76 (MQ=255)                                                                                                                                  cGTGAGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTcg                                                                <  2:825097/76‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      GGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTTGCCAGGACGCAAAAATATTATCCTCAGCAGTCAACCGGGTACGGACGATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAG  >  NC_000913/49911‑50177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑