Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 758,907 C→T R68C (CGC→TGC)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913758,9070CT100.0% 255.8 / NA 79R68C (CGC→TGC) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/37);  total (42/37)

GCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCCGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGC  >  NC_000913/758780‑759039
                                                                                                                               |                                                                                                                                    
gCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGaaa                                                                                                                           <  2:114128/139‑1 (MQ=255)
 ctctATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAg                                                                                                                          >  2:873831/1‑139 (MQ=255)
    tATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACg                                                                                                                       <  1:1307590/139‑1 (MQ=255)
           aCTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACg                                                                                                                >  2:1493550/1‑139 (MQ=255)
                 tAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTActc                                                                                                          <  1:873831/139‑1 (MQ=255)
                      gATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCaa                                                                                                     <  2:487469/139‑1 (MQ=255)
                       aTCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTa                                                                                                             >  1:1466360/1‑130 (MQ=255)
                       aTCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTa                                                                                                             >  2:446227/1‑130 (MQ=255)
                       aTCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTa                                                                                                             <  1:446227/130‑1 (MQ=255)
                       aTCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAAc                                                                                                    >  1:563958/1‑139 (MQ=255)
                       aTCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAAc                                                                                                    >  1:1254966/1‑139 (MQ=255)
                       aTCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAAc                                                                                                    >  1:1891132/1‑139 (MQ=255)
                       aTCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAAc                                                                                                    >  2:1663737/1‑139 (MQ=255)
                       aTCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCAAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTa                                                                                                             <  2:1466360/130‑1 (MQ=255)
                         ccTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCaa                                                                                                     >  2:445773/1‑136 (MQ=255)
                         ccTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCaa                                                                                                     <  1:445773/136‑1 (MQ=255)
                         ccTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGa                                                                                                  >  1:930394/1‑139 (MQ=255)
                             aCTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTa                                                                                                             >  1:1106471/1‑124 (MQ=255)
                             aCTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTa                                                                                                             <  2:1106471/124‑1 (MQ=255)
                              cTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGa                                                                                                                         >  2:1359011/1‑111 (MQ=255)
                              cTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGa                                                                                                                         <  1:1359011/111‑1 (MQ=255)
                                  gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTa                                                                                                             >  2:1591743/1‑119 (MQ=255)
                                  gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTa                                                                                                             <  1:1591743/119‑1 (MQ=255)
                                  gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAAc                                                                                                    >  2:1115693/1‑128 (MQ=255)
                                  gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAAc                                                                                                    <  1:1115693/128‑1 (MQ=255)
                                  gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGAcc                                                                                         >  2:743931/1‑139 (MQ=255)
                                  gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGAcc                                                                                         >  2:728683/1‑139 (MQ=255)
                                  gTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGAcc                                                                                         >  1:598884/1‑139 (MQ=255)
                                    tGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCt                                                                                       <  1:758708/139‑1 (MQ=255)
                                              tGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGAGCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAAtg                                                                             <  1:1663737/139‑1 (MQ=255)
                                                 cGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGa                                                                          >  2:953653/1‑139 (MQ=255)
                                                   ttCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAAc                                                                                                    <  2:858661/111‑1 (MQ=255)
                                                   ttCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAAc                                                                                                    >  1:858661/1‑111 (MQ=255)
                                                   ttCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCTGACCCTGACACCAATGTGAAg                                                                        <  1:1493550/139‑1 (MQ=255)
                                                   ttCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAg                                                                        <  2:930394/139‑1 (MQ=255)
                                                   ttCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCCGACACCAATGTGAAg                                                                        <  2:1623033/139‑1 (MQ=255)
                                                    tCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTcc                                                                                             >  2:918359/1‑117 (MQ=255)
                                                    tCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTcc                                                                                             <  1:918359/117‑1 (MQ=255)
                                                        cGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCt                                                                                                                     <  1:1643483/89‑1 (MQ=255)
                                                        cGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCt                                                                                                                     >  2:1643483/1‑89 (MQ=255)
                                                                  gTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTg                                                         <  1:728683/139‑1 (MQ=255)
                                                                    tACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCa                                                       >  2:1809614/1‑139 (MQ=255)
                                                                     aCCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTcc                                                                                             <  2:2048988/100‑1 (MQ=255)
                                                                     aCCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTcc                                                                                             >  1:2048988/1‑100 (MQ=255)
                                                                     aCCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAg                                                      <  2:1254966/139‑1 (MQ=255)
                                                                     aCCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAg                                                      >  2:567664/1‑139 (MQ=255)
                                                                     aCCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAg                                                      <  1:743931/139‑1 (MQ=255)
                                                                               ggAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACg                                            >  2:1204982/1‑139 (MQ=255)
                                                                                   tCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGt                                                                                                               <  1:1727158/68‑1 (MQ=255)
                                                                                   tCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGt                                                                                                               >  2:1727158/1‑68 (MQ=255)
                                                                                    cAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCTGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCa                                                  >  1:933577/1‑128 (MQ=255)
                                                                                    cAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCTGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCa                                                  <  2:933577/128‑1 (MQ=255)
                                                                                    cAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGaa                                                                         <  2:838486/105‑1 (MQ=255)
                                                                                    cAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGaa                                                                         >  1:838486/1‑105 (MQ=255)
                                                                                    cAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATAc                                       >  2:1965756/1‑139 (MQ=255)
                                                                                                   ccACTCTCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCAc                        <  2:598884/139‑1 (MQ=255)
                                                                                                      ctctCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCa                                                                                                                  <  2:1565531/46‑1 (MQ=255)
                                                                                                      ctctCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCa                                                                                                                  >  1:1565531/1‑46 (MQ=255)
                                                                                                      ctctCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGacac                                                                                  <  2:1555688/78‑1 (MQ=255)
                                                                                                      ctctCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGacac                                                                                  >  1:1555688/1‑78 (MQ=255)
                                                                                                      ctctCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAg                     <  2:1891132/139‑1 (MQ=255)
                                                                                                      ctctCAAACGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGA‑CCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGc                    >  1:1982873/1‑139 (MQ=255)
                                                                                                           aaaCGCGTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGc                >  1:2007572/1‑139 (MQ=255)
                                                                                                                cgTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGa                                                                                     <  2:519740/65‑1 (MQ=255)
                                                                                                                cgTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGa                                                                                     >  1:519740/1‑65 (MQ=255)
                                                                                                                cgTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAg                                                                        <  2:1872922/78‑1 (MQ=255)
                                                                                                                cgTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAg                                                                        >  1:1872922/1‑78 (MQ=255)
                                                                                                                cgTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATAc                                       <  2:1746982/111‑1 (MQ=255)
                                                                                                                cgTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATAc                                       >  1:1746982/1‑111 (MQ=255)
                                                                                                                cgTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCg                               >  2:1774621/1‑119 (MQ=255)
                                                                                                                cgTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCg                               <  1:1774621/119‑1 (MQ=255)
                                                                                                                 gTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGTAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAAtct          >  2:631171/1‑139 (MQ=255)
                                                                                                                 gTGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAAtct          >  2:1695915/1‑139 (MQ=255)
                                                                                                                  tGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCaa                                                                                                     >  1:1434125/1‑47 (MQ=255)
                                                                                                                  tGAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCaa                                                                                                     <  2:1434125/47‑1 (MQ=255)
                                                                                                                   gAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAg                                                                        >  2:646169/1‑75 (MQ=255)
                                                                                                                   gAATATTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAg                                                                        <  1:646169/75‑1 (MQ=255)
                                                                                                                     atatTTCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGAt      <  2:563958/139‑1 (MQ=255)
                                                                                                                         ttCCGCTGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGc  >  1:652968/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                    
GCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTGGCGTTCGACGTTCCAGCAGTTACCTGGTACGGGAGTCAAACCGGATCAATTCCACTCTCAAACGCGTGAATATTTCCGCCGCCTGGCGAAAGACGCTTCACGTTACTCTTCAACGATCTCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGC  >  NC_000913/758780‑759039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑