Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	75,447	A→G	C12R (TGC→CGC)	thiB ←	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,447	0	A	G	100.0%	221.5 / NA	61	C12R (TGC→CGC)	thiB	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/28);  total (33/28)

CGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCA  >  NC_000913/75313‑75580                                                                                                                                       |                                                                                                                                      cgcCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagc                                                                                                                                   <  1:214121/139‑1 (MQ=255)               gTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAg                                                                                                                     >  2:1743507/1‑139 (MQ=255)                   gCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                                                 <  1:508230/139‑1 (MQ=255)                    cAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAtt                                                                                                                >  1:133874/1‑139 (MQ=255)                    cAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAtt                                                                                                                >  1:137838/1‑139 (MQ=255)                             tCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagca                                                                                                                                  <  2:679387/112‑1 (MQ=255)                             tCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagca                                                                                                                                  >  1:679387/1‑112 (MQ=255)                                 cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACttt                                                                                                   >  2:510673/1‑139 (MQ=255)                                 cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACttt                                                                                                   >  2:1686816/1‑139 (MQ=255)                                 cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACttt                                                                                                   >  2:1891678/1‑139 (MQ=255)                                 cTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTAAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACttt                                                                                                   >  2:1262084/1‑139 (MQ=255)                                    cAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                                <  1:1262084/139‑1 (MQ=255)                                                 aaCCACCGGACCACGCCCCCAGTCGGCGGCGAAGTAATCGTAGGTATAAACAGTCAGAACGTGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                                   <  1:50628/139‑1 (MQ=255)                                                   ccaccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTaa                                                                                                         >  1:406290/1‑115 (MQ=255)                                                   ccaccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTaa                                                                                                         <  2:406290/115‑1 (MQ=255)                                                   ccaccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTg                                                                                 >  1:1519224/1‑139 (MQ=255)                                                      ccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa                                                                              <  1:1686816/139‑1 (MQ=255)                                                      ccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCa                                                                              <  2:1519224/139‑1 (MQ=255)                                                       cGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                             <  1:1743507/139‑1 (MQ=255)                                                       cGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                             <  1:1362869/139‑1 (MQ=255)                                                         gACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACTGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagcaacag                                                                                                                              >  2:1377041/1‑88 (MQ=255)                                                         gACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCgcagcaacag                                                                                                                              <  1:1377041/88‑1 (MQ=255)                                                              ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCaaaa                                                                                        <  2:2037421/121‑1 (MQ=255)                                                              ggCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCaaaa                                                                                        >  1:2037421/1‑121 (MQ=255)                                                                   ccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGaa                                                                 >  1:1052513/1‑139 (MQ=255)                                                                         ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                                                 >  2:92716/1‑85 (MQ=255)                                                                         ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                                                 <  1:92716/85‑1 (MQ=255)                                                                         ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                                <  2:1655324/102‑1 (MQ=255)                                                                         ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                                >  1:1770233/1‑102 (MQ=255)                                                                         ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                                >  1:1655324/1‑102 (MQ=255)                                                                         ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                                <  2:1770233/102‑1 (MQ=255)                                                                         ggcggcGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGcc                                                           >  2:357062/1‑139 (MQ=255)                                                                           cggcgAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTc                                                         >  1:515729/1‑139 (MQ=255)                                                                             gcgAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCaa                                                       <  1:548868/139‑1 (MQ=255)                                                                                     aTCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                  <  2:161180/88‑1 (MQ=255)                                                                                     aTCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTg                                                                                                  >  1:161180/1‑88 (MQ=255)                                                                                            gTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCg                                        <  1:357062/139‑1 (MQ=255)                                                                                             tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                                                 <  1:88223/65‑1 (MQ=255)                                                                                             tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACAt                                                                                                                 >  2:88223/1‑65 (MQ=255)                                                                                               taAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTa                                     <  1:1891678/139‑1 (MQ=255)                                                                                                 aaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAg                                                                >  1:1238165/1‑110 (MQ=255)                                                                                                 aaCAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAg                                                                <  2:1238165/110‑1 (MQ=255)                                                                                                      tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGTACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                              >  1:1011926/1‑139 (MQ=255)                                                                                                      tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                                <  1:902318/73‑1 (MQ=255)                                                                                                      tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCa                                                                                                >  2:902318/1‑73 (MQ=255)                                                                                                      tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                                   >  2:9414/1‑86 (MQ=255)                                                                                                      tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAagag                                                                                   <  1:9414/86‑1 (MQ=255)                                                                                                      tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                              >  2:1431928/1‑139 (MQ=255)                                                                                                      tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGAt                              >  2:1797134/1‑139 (MQ=255)                                                                                                       cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                             >  1:1810938/1‑139 (MQ=255)                                                                                                       cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATc                             >  1:630678/1‑139 (MQ=255)                                                                                                           aCGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGt                         <  2:1810938/139‑1 (MQ=255)                                                                                                            cGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCg                                              >  2:64341/1‑117 (MQ=255)                                                                                                            cGGGTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCg                                              <  1:64341/117‑1 (MQ=255)                                                                                                              ggTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCg                      >  2:1004367/1‑139 (MQ=255)                                                                                                              ggTTTAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCg                      >  1:250716/1‑139 (MQ=255)                                                                                                                tttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                             <  2:1163802/82‑1 (MQ=255)                                                                                                                tttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCaa                                                                             >  1:1163802/1‑82 (MQ=255)                                                                                                                tttAGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGAc                    >  2:1115564/1‑139 (MQ=255)                                                                                                                   aGCGAAAACGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACggg                 <  1:1115564/139‑1 (MQ=255)                                                                                                                           cGGGCGCTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTtct         <  1:510673/139‑1 (MQ=255)                                                                                                                              gcgcTGTGCGCAGCAGCAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                               <  2:1831655/82‑1 (MQ=255)                                                                                                                              gcgcTGTGCGCAGCAGCAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAgg                                                               >  1:1831655/1‑82 (MQ=255)                                                                                                                                 cTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGTACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcgc   <  2:1011926/139‑1 (MQ=255)                                                                                                                                 cTGTGCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAgcgc   <  1:1797134/139‑1 (MQ=255)                                                                                                                                  tgtgCGCAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCa  >  1:819750/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      CGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCA  >  NC_000913/75313‑75580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑