Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	927,205	A→G	R76R (CGT→CGC)	aat ←	leucyl/phenylalanyl‑tRNA‑protein transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	927,205	0	A	G	100.0%	209.9 / NA	60	R76R (CGT→CGC)	aat	leucyl/phenylalanyl‑tRNA‑protein transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/33);  total (27/33)

AGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGC  >  NC_000913/927075‑927327                                                                                                                                   |                                                                                                                           aGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGat                                                                                                                    <  2:177912/139‑1 (MQ=255)  ggCTTCGACCACGCCAGGCGTGTTCCAGGTTCCTTCTGCGCGATCGCTTCCACAGCCTTTAATGACCTGTCCAAAAGCCTACTTCATCGGAAAACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCAGCTGaaa                                                                                                                   <  1:765308/139‑3 (MQ=255)       cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACATCCGTCAATGACCTGACCAAAAGCGTAATTCATCGTCACACGATAGAGCGAGCGTTTATGAAATTTCTTCATACTGCGGCTGATATGCAg                                                                                                              <  1:1977275/139‑1 (MQ=255)       cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAGTGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              <  1:104073/139‑1 (MQ=255)       cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGata                                                                                                                   <  2:53328/134‑1 (MQ=255)       cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGata                                                                                                                   >  1:53328/1‑134 (MQ=255)       cGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              >  1:314595/1‑139 (MQ=255)             cgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTc                                                                                                        >  2:873529/1‑139 (MQ=255)             cgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTc                                                                                                        >  1:1899937/1‑139 (MQ=255)                        aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                         <  2:1911892/127‑1 (MQ=255)                        aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                         >  1:1911892/1‑127 (MQ=255)                             ggTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACcgcg                                                                                        >  1:1705106/1‑139 (MQ=255)                                   ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGat                                                                                                                    >  2:325119/1‑105 (MQ=255)                                   ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGat                                                                                                                    <  1:325119/105‑1 (MQ=255)                                    tcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              <  2:166651/110‑1 (MQ=255)                                    tcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              >  1:166651/1‑110 (MQ=255)                                        cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCggg                                                                             >  1:1790008/1‑139 (MQ=255)                                        cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCggg                                                                             <  2:1899937/139‑1 (MQ=255)                                         gcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGc                                                                            <  2:1948920/139‑1 (MQ=255)                                          cgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCg                                                                           <  2:290182/139‑1 (MQ=255)                                            aTCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac                                                                         <  1:873529/139‑1 (MQ=255)                                                 tGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGAccacca                                                                    <  2:314595/139‑1 (MQ=255)                                                         ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCt                                                                                                       <  1:386718/96‑1 (MQ=255)                                                         ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCt                                                                                                       >  2:386718/1‑96 (MQ=255)                                                         ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCg                                                                           <  1:645549/124‑1 (MQ=255)                                                         ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCg                                                                           >  2:645549/1‑124 (MQ=255)                                                          cTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATggg                                                           >  2:252781/1‑139 (MQ=255)                                                              aaTGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGc                                                                            <  2:1986159/118‑1 (MQ=255)                                                              aaTGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGc                                                                            >  1:1986159/1‑118 (MQ=255)                                                               aTGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGc                                                      <  2:1705106/139‑1 (MQ=255)                                                                   cctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                         >  2:947408/1‑84 (MQ=255)                                                                   cctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                         <  1:947408/84‑1 (MQ=255)                                                                       accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac                                                                         <  2:1108406/112‑1 (MQ=255)                                                                       accAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGac                                                                         >  1:1108406/1‑112 (MQ=255)                                                                        ccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCa                                                                                             >  2:577957/1‑91 (MQ=255)                                                                        ccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCa                                                                                             <  1:577957/91‑1 (MQ=255)                                                                                gTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGa                                     <  1:252781/139‑1 (MQ=255)                                                                                 tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCgg                                                                                                                         <  2:1281051/54‑1 (MQ=255)                                                                                 tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCgg                                                                                                                         >  1:1281051/1‑54 (MQ=255)                                                                                 tAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGaa                                    >  1:898792/1‑139 (MQ=255)                                                                                              cacGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              >  2:1855053/1‑52 (MQ=255)                                                                                              cacGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAg                                                                                                              <  1:1855053/52‑1 (MQ=255)                                                                                                cGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGTGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                     <  1:1340679/139‑1 (MQ=255)                                                                                                cGATAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                     <  2:1849686/139‑1 (MQ=255)                                                                                                   tAGGGCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAg                  >  2:891562/1‑139 (MQ=255)                                                                                                     gggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGcc                >  1:1908209/1‑139 (MQ=255)                                                                                                      ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                     <  2:1195130/133‑1 (MQ=255)                                                                                                      ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                     >  1:1195130/1‑133 (MQ=255)                                                                                                      ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAg                  >  2:1191301/1‑136 (MQ=255)                                                                                                      ggCGAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAg                  <  1:1191301/136‑1 (MQ=255)                                                                                                         gAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                         >  1:1005816/1‑46 (MQ=255)                                                                                                         gAGCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGAtt                                                                                                         <  2:1005816/46‑1 (MQ=255)                                                                                                           gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGTGGATCGGGCGACCACGAGAGGATGGGGTCGCCTGGAGAAAACCAc                                        <  1:1188937/109‑1 (MQ=255)                                                                                                           gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCAc                                        >  2:1188937/1‑109 (MQ=255)                                                                                                           gCGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAAc          >  1:1123525/1‑139 (MQ=255)                                                                                                            cGTTTATGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACa         >  2:1428943/1‑139 (MQ=255)                                                                                                                  tGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg   <  2:1790008/139‑1 (MQ=255)                                                                                                                  tGAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg   <  2:898792/139‑1 (MQ=255)                                                                                                                   gAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc  <  1:135228/139‑1 (MQ=255)                                                                                                                   gAAATCGCTTCATACTGCGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc  <  1:891562/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                           AGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGC  >  NC_000913/927075‑927327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑