Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 969,352 G→A *329* (TAG→TAA lpxK → lipid A 4'kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913969,3520GA100.0% 228.9 / NA 70*329* (TAG→TAAlpxKlipid A 4'kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/36);  total (34/36)

TGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAGGA  >  NC_000913/969220‑969486
                                                                                                                                    |                                                                                                                                      
tGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTAcgc                                                                                                                                  >  1:1601483/1‑139 (MQ=255)
  aaaaaGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTAcgccg                                                                                                                                <  2:2017840/139‑1 (MQ=255)
  aaaaaGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTAcgccg                                                                                                                                >  1:2017840/1‑139 (MQ=255)
                     ccGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                                             <  1:689439/139‑1 (MQ=255)
                     ccGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                                             <  1:1664922/139‑1 (MQ=255)
                      cGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATgg                                                                                                            <  1:1925956/139‑1 (MQ=255)
                        ggCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAg                                                                                                          >  2:19885/1‑139 (MQ=255)
                             ttGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATg                                                                                                     >  1:1990129/1‑139 (MQ=255)
                             ttGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATg                                                                                                     <  1:1296922/139‑1 (MQ=255)
                              tGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGa                                                                                                    >  2:1809931/1‑139 (MQ=255)
                              tGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGa                                                                                                    <  2:1171129/139‑1 (MQ=255)
                                          ttGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCg                                                                                        <  1:1943460/139‑1 (MQ=255)
                                             gtggtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAc                                                                                     >  2:1895491/1‑139 (MQ=255)
                                               ggtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACct                                                                                   >  1:932216/1‑139 (MQ=255)
                                                gtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGa                                                                                                           >  2:1691988/1‑114 (MQ=255)
                                                gtATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGa                                                                                                           <  1:1691988/114‑1 (MQ=255)
                                                          gTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGcc                                                                                         >  1:1441512/1‑122 (MQ=255)
                                                          gTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGcc                                                                                         <  2:1441512/122‑1 (MQ=255)
                                                           tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGt                                                                                                                       <  1:1822848/91‑1 (MQ=255)
                                                           tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGt                                                                                                                       >  2:1822848/1‑91 (MQ=255)
                                                           tAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGa                                                                       >  1:848975/1‑139 (MQ=255)
                                                                 cacaGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGcgcg                                                                 <  2:848975/139‑1 (MQ=255)
                                                                     gCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTGATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAAt                                                             >  1:1335917/1‑139 (MQ=255)
                                                                      cTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATc                                                            >  1:1811189/1‑139 (MQ=255)
                                                                           aGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCg                                                                                        >  1:99291/1‑106 (MQ=255)
                                                                           aGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCg                                                                                        <  2:99291/106‑1 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                                             >  2:1125140/1‑80 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                                             <  1:2019501/80‑1 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                                             >  2:2019501/1‑80 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATg                                                                                                             <  1:1125140/80‑1 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGc                                                                                          <  2:1265090/99‑1 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGc                                                                                          >  1:1265090/1‑99 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAcc                                                                                    >  2:510178/1‑105 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCAcc                                                                                    <  1:510178/105‑1 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGc                                                  >  1:1231369/1‑139 (MQ=255)
                                                                                atgaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGc                                                  <  2:1231369/139‑1 (MQ=255)
                                                                                  gaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGcc                                                                                         <  1:1377857/98‑1 (MQ=255)
                                                                                  gaACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGcc                                                                                         >  2:1377857/1‑98 (MQ=255)
                                                                                        gCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCt                                                     <  1:951404/128‑1 (MQ=255)
                                                                                        gCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCt                                                     >  2:951404/1‑128 (MQ=255)
                                                                                        gCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAg                                          <  2:932216/139‑1 (MQ=255)
                                                                                        gCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAg                                          >  2:374589/1‑139 (MQ=255)
                                                                                        gCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAg                                          <  1:1118900/139‑1 (MQ=255)
                                                                                         cGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAgg                                         >  1:1512495/1‑139 (MQ=255)
                                                                                          gAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGc                                        >  2:1919733/1‑139 (MQ=255)
                                                                                          gAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGc                                        <  2:128345/139‑1 (MQ=255)
                                                                                          gAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGc                                        <  2:1811189/139‑1 (MQ=255)
                                                                                            aaCTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCt                                      <  2:1601483/139‑1 (MQ=255)
                                                                                                        aaCTAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAAcc                          <  2:1512495/139‑1 (MQ=255)
                                                                                                           tAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGt                                                                                                                       <  2:1045713/43‑1 (MQ=255)
                                                                                                           tAACCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGt                                                                                                                       >  1:1045713/1‑43 (MQ=255)
                                                                                                             aCCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACctc                                                                                  <  1:1095621/78‑1 (MQ=255)
                                                                                                             aCCTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACctc                                                                                  >  2:1095621/1‑78 (MQ=255)
                                                                                                               cTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGc                                                                                             <  1:945421/65‑1 (MQ=255)
                                                                                                               cTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGc                                                                                             >  2:945421/1‑65 (MQ=255)
                                                                                                               cTTGCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGt                   >  1:122555/1‑139 (MQ=255)
                                                                                                                  gCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGAt                                                                      >  2:1598268/1‑85 (MQ=255)
                                                                                                                  gCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGAt                                                                      <  1:1598268/85‑1 (MQ=255)
                                                                                                                  gCTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAAAAAACCCCGCCGTCGa                >  1:493408/1‑139 (MQ=255)
                                                                                                                   cTGGCTTCTGGTAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCTCCGTCGAg               <  2:1335917/139‑1 (MQ=255)
                                                                                                                   cTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAg                                          <  1:985514/112‑1 (MQ=255)
                                                                                                                   cTGGCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAg                                          >  2:985514/1‑112 (MQ=255)
                                                                                                                      gCTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGcgt            <  1:1809931/139‑1 (MQ=255)
                                                                                                                       cTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATgg                                                                                                            <  1:530888/42‑1 (MQ=255)
                                                                                                                       cTTCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATgg                                                                                                            >  2:530888/1‑42 (MQ=255)
                                                                                                                        ttCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGcgcg                                                                 >  2:1434407/1‑84 (MQ=255)
                                                                                                                        ttCTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGcgcg                                                                 <  1:1434407/84‑1 (MQ=255)
                                                                                                                          cTGGCAACTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGtt        <  1:1173810/139‑1 (MQ=255)
                                                                                                                                aCTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCGTGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTggagga  >  2:93980/1‑139 (MQ=255)
                                                                                                                                 cTAATTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGc                                        <  1:1731240/100‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
TGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGGTGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAACTAACCTTGCTGGCTTCTGGCAACTAGTTACGCCGCGGCAGCGTTCGATTGATGGAGTCATGAATGTCGCTGCCGCACCTCTCCCTTGCTGATGCGCGTAATCTTCACCTTGCCGCACAAGGCCTGTTAAACAAACCCCGCCGTCGAGCGTCGTTGGAGGA  >  NC_000913/969220‑969486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: