Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,125,011	A→G	C106R (TGC→CGC)	mdtH ←	multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,125,011	0	A	G	100.0%	216.7 / NA	60	C106R (TGC→CGC)	mdtH	multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/28);  total (32/28)

TGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGAC  >  NC_000913/1124879‑1125145                                                                                                                                      |                                                                                                                                       tGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGaaaa                                                                                                                                  <  2:446325/139‑1 (MQ=255)        aCCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAAt                                                                                                                           >  1:247830/1‑139 (MQ=255)                     tGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGt                                                                                                              <  2:2040310/139‑1 (MQ=255)                            tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACTGATTAATTTCGCCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACTGAGTCCCGAGAGCAGGCGTGAAAAc                                                                                                                                 >  2:1308926/1‑113 (MQ=255)                            tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAc                                                                                                                                 <  1:1308926/113‑1 (MQ=255)                            tcaACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                       >  1:832643/1‑139 (MQ=255)                                     agaAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                       <  2:295310/130‑1 (MQ=255)                                     agaAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGAt                                                                                                       >  1:295310/1‑130 (MQ=255)                                        aaaaaCGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGc                                                                                           >  1:367675/1‑139 (MQ=255)                                                ccACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCcgg                                                                                   >  2:1715497/1‑139 (MQ=255)                                                       gctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGgcgcg                                                                            <  2:367675/139‑1 (MQ=255)                                                       gctgTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGgcgcg                                                                            <  2:1801440/139‑1 (MQ=255)                                                                ggATTAATTTCACCACCAGCGCCGAACGCGGCGGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGt                                                                                                              <  1:1910334/97‑1 (MQ=255)                                                                ggATTAATTTCACCACCAGCGCCGAACGCGGCGGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGt                                                                                                              >  2:1910334/1‑97 (MQ=255)                                                                  aTTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                              <  2:691385/126‑1 (MQ=255)                                                                  aTTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                              >  1:691385/1‑126 (MQ=255)                                                                  aTTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATAc                                                                 <  2:776856/139‑1 (MQ=255)                                                                            ccaccaGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAat                                                       >  2:1887968/1‑139 (MQ=255)                                                                                 aGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCcggcgg                                                                                <  2:1910183/109‑1 (MQ=255)                                                                                 aGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCcggcgg                                                                                >  1:1910183/1‑109 (MQ=255)                                                                                  gcgcCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAc                                                                                                                                 <  1:472097/59‑1 (MQ=255)                                                                                  gcgcCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAc                                                                                                                                 >  2:472097/1‑59 (MQ=255)                                                                                    gcCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGtt                                               <  2:1334900/139‑1 (MQ=255)                                                                                     ccGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                              >  1:339296/1‑107 (MQ=255)                                                                                     ccGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                              <  2:339296/107‑1 (MQ=255)                                                                                     ccGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGttt                                              >  2:941029/1‑139 (MQ=255)                                                                                       gAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCCGGCGTGAAAACCACAATAGc                                                                                                                        <  1:1207720/63‑1 (MQ=255)                                                                                       gAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGc                                                                                                                        >  2:1207720/1‑63 (MQ=255)                                                                                        aaCGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAcc                                                                                                                                <  2:614211/54‑1 (MQ=255)                                                                                        aaCGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAAcc                                                                                                                                >  1:614211/1‑54 (MQ=255)                                                                                             ggc‑ggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCAtt                                                                                                >  1:662659/1‑81 (MQ=255)                                                                                             ggc‑ggATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCAtt                                                                                                <  2:662659/81‑1 (MQ=255)                                                                                                            cGTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAAtt                        >  1:1821041/1‑139 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGATCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc                                                                                                          <  2:304116/56‑1 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGt                                                                                                              <  2:1318280/52‑1 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGt                                                                                                              >  1:1318280/1‑52 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc                                                                                                          >  1:304116/1‑56 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGgcgcg                                                                            >  1:100771/1‑86 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGgcgcg                                                                            <  2:100771/86‑1 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt                                                                         >  1:1881778/1‑89 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt                                                                         <  2:1881778/89‑1 (MQ=255)                                                                                                             gTGCCACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTg                       >  2:1165993/1‑139 (MQ=255)                                                                                                                 cACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTaa                                                           >  2:145626/1‑99 (MQ=255)                                                                                                                 cACCGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTaa                                                           <  1:145626/99‑1 (MQ=255)                                                                                                                   ccGAGTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCAccgc                 <  2:1821041/139‑1 (MQ=255)                                                                                                                       gTCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGaa             <  2:247830/139‑1 (MQ=255)                                                                                                                        tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc                                                                                                          <  1:140443/45‑1 (MQ=255)                                                                                                                        tCCCGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGc                                                                                                          >  2:140443/1‑45 (MQ=255)                                                                                                                           cGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCg                                                                                                         >  1:635046/1‑43 (MQ=255)                                                                                                                           cGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCg                                                                                                         <  2:635046/43‑1 (MQ=255)                                                                                                                           cGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt                                                                         <  2:1909176/75‑1 (MQ=255)                                                                                                                           cGAGAGCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCAt                                                                         >  1:1909176/1‑75 (MQ=255)                                                                                                                             agagCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATAcc       >  1:558021/1‑139 (MQ=255)                                                                                                                                gCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                              >  2:1030743/1‑65 (MQ=255)                                                                                                                                gCAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                              <  1:1030743/65‑1 (MQ=255)                                                                                                                                 cAGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGa   >  1:2018527/1‑139 (MQ=255)                                                                                                                                  aGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGAc  >  1:1569710/1‑139 (MQ=255)                                                                                                                                  aGGCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGAc  >  2:1477633/1‑139 (MQ=255)                                                                                                                                   ggCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                              >  2:265/1‑62 (MQ=255)                                                                                                                                   ggCGTGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                              <  1:265/62‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCACGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGC‑GGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGAAAATACCCAGAC  >  NC_000913/1124879‑1125145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑