Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,167,693	C→T	P32L (CCG→CTG)	ycfJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,167,693	0	C	T	100.0%	211.5 / NA	65	P32L (CCG→CTG)	ycfJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/32);  total (33/32)

TGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCG  >  NC_000913/1167565‑1167827                                                                                                                                 |                                                                                                                                       tGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              <  2:179994/139‑1 (MQ=255)    ttGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              >  1:463460/1‑136 (MQ=255)    ttGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              <  2:463460/136‑1 (MQ=255)                     aagGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcccc                                                                                                          <  1:731879/139‑1 (MQ=255)                       ggaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCaa                                                                                                                                    >  1:163597/1‑111 (MQ=255)                       ggaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCaa                                                                                                                                    <  2:163597/111‑1 (MQ=255)                        gaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAAt                                                                                                       >  2:1540753/1‑139 (MQ=255)                        gaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAAt                                                                                                       >  2:1997305/1‑139 (MQ=255)                        gaggTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAAt                                                                                                       >  2:1746840/1‑139 (MQ=255)                           gtgtTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                          >  1:234795/1‑117 (MQ=255)                           gtgtTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                          <  2:234795/117‑1 (MQ=255)                               tCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              >  1:1833194/1‑109 (MQ=255)                               tCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCt                                                                                                                              <  2:1833194/109‑1 (MQ=255)                               tCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGaa                                                                                                >  2:251306/1‑139 (MQ=255)                               tCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGaa                                                                                                >  2:1612690/1‑139 (MQ=255)                                           aTCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGCACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAaca                                                                                    >  2:1283094/1‑139 (MQ=255)                                           aTCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCa                                                                                                               <  1:170104/112‑1 (MQ=255)                                           aTCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCa                                                                                                               >  2:170104/1‑112 (MQ=255)                                            tCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   <  1:1746840/139‑1 (MQ=255)                                            tCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   <  1:251306/139‑1 (MQ=255)                                            tCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   <  1:474738/139‑1 (MQ=255)                                                tGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACcgcg                                                                               >  1:2054953/1‑139 (MQ=255)                                                tGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACcgcg                                                                               >  1:924593/1‑139 (MQ=255)                                                     gCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGgttgt                                                                                                                      <  1:306610/95‑1 (MQ=255)                                                     gCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGgttgt                                                                                                                      >  2:306610/1‑95 (MQ=255)                                                          tATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                            <  1:1714397/100‑1 (MQ=255)                                                          tATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcc                                                                                                            >  2:1714397/1‑100 (MQ=255)                                                          tATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   >  2:893336/1‑125 (MQ=255)                                                          tATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAacac                                                                                   <  1:893336/125‑1 (MQ=255)                                                            tCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAcccc                                                                                                          <  1:1251703/100‑1 (MQ=255)                                                            tCGGGATTGGTGTCACAGCTGCGCTGGGCGTAGCGGCAGTAGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAAccct                                                                                                          >  2:1251703/1‑99 (MQ=255)                                                                gATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAAc                                                               <  1:1997305/139‑1 (MQ=255)                                                                  ttGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGt                                                             <  2:924593/139‑1 (MQ=255)                                                                  ttGGTGTCGCAGCTGCGCTGCGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGt                                                             <  1:595852/139‑1 (MQ=255)                                                                   tGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTc                                                            >  1:1543775/1‑139 (MQ=255)                                                                    ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTg                                                                                                                 >  2:945224/1‑85 (MQ=255)                                                                    ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTg                                                                                                                 <  1:945224/85‑1 (MQ=255)                                                                    ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAg                                                                           <  2:775272/123‑1 (MQ=255)                                                                    ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAg                                                                           >  1:775272/1‑123 (MQ=255)                                                                    ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTca                                                           >  2:1920063/1‑139 (MQ=255)                                                                    ggTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTca                                                           >  2:1855056/1‑139 (MQ=255)                                                                         cGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                          <  2:616176/71‑1 (MQ=255)                                                                         cGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAgg                                                                                                                          >  1:616176/1‑71 (MQ=255)                                                                           cAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGAc                                                    >  2:1602836/1‑139 (MQ=255)                                                                            aGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGAcc                                                   <  2:1118402/139‑1 (MQ=255)                                                                                gcgcTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAg                                                                           >  2:1258194/1‑111 (MQ=255)                                                                                gcgcTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAg                                                                           <  1:1258194/111‑1 (MQ=255)                                                                                   cTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCAtcgtc                                            <  1:1283094/139‑1 (MQ=255)                                                                                            gCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGc                                   <  1:1920063/139‑1 (MQ=255)                                                                                                    ggCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaa                           <  1:1612690/139‑1 (MQ=255)                                                                                                     gCCAGTCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGaaa                          >  2:1141013/1‑139 (MQ=255)                                                                                                          tCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTcaca                                                         <  1:1153336/103‑1 (MQ=255)                                                                                                          tCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTcaca                                                         >  2:1153336/1‑103 (MQ=255)                                                                                                          tCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCa                                  <  1:62166/126‑1 (MQ=255)                                                                                                          tCTGAACGTGTTTGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCa                                  >  2:62166/1‑126 (MQ=255)                                                                                                                    tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGc                                                                  <  2:1927709/84‑1 (MQ=255)                                                                                                                    tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGc                                                                  >  1:1927709/1‑84 (MQ=255)                                                                                                                    tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCa                                                                 >  1:39394/1‑85 (MQ=255)                                                                                                                    tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCa                                                                 <  2:39394/85‑1 (MQ=255)                                                                                                                    tttGAACGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGt           <  1:1540753/139‑1 (MQ=255)                                                                                                                        aaCGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGt                                                       <  2:325778/91‑1 (MQ=255)                                                                                                                        aaCGGGGCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGt                                                       >  1:325778/1‑91 (MQ=255)                                                                                                                           ggggCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCt    <  2:1029636/139‑1 (MQ=255)                                                                                                                             ggCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCg  >  2:1498762/1‑139 (MQ=255)                                                                                                                             ggCCTGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGAGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCg  >  1:31188/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       TGATTGGCATAACTGCAAAGAAGGAGGTGTTCCCGTGAATAAATCAATGTTGGCGGGTATCGGGATTGGTGTCGCAGCTGCGCTGGGCGTAGCGGCAGTGGCCAGTCTGAACGTGTTTGAACGGGGCCCGCAATACGCTCAGGTTGTTTCTGCAACCCCAATCAAGGAAACGGTTAAAACACCGCGTCAGGAGTGTCGCAACGTCACAGTGACCCATCGTCGACCGGTGCAGGATGAAAATCGCATTACCGGGTCGGTGCTCG  >  NC_000913/1167565‑1167827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑