Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,228,724	A→G	T216A (ACT→GCT)	ycgM →	putative isomerase/hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,228,724	0	A	G	100.0%	170.4 / NA	48	T216A (ACT→GCT)	ycgM	putative isomerase/hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

AGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTA  >  NC_000913/1228607‑1228857                                                                                                                      |                                                                                                                                      aGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACtttt                                                                                                                  >  2:254363/1‑139 (MQ=255) aGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTC‑TTGACAGCTCGCGTTTTGTAATAAttttt                                                                                                                 >  2:1279143/1‑139 (MQ=255)   cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcgcg                                                                                                                                 >  2:1712875/1‑122 (MQ=255)   cAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcgcg                                                                                                                                 <  1:1712875/122‑1 (MQ=255)                    aGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGcggcgg                                                                                               <  2:1222525/139‑1 (MQ=255)                         ggTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGtt                                                                                                                               <  2:394714/102‑1 (MQ=255)                         ggTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGtt                                                                                                                               >  1:394714/1‑102 (MQ=255)                         ggTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTgc                                                                                                               >  2:1945890/1‑118 (MQ=255)                         ggTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTgc                                                                                                               <  1:1945890/118‑1 (MQ=255)                         ggTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGTGGCaaaa                                                                                          >  1:236458/1‑139 (MQ=255)                           tGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACt                                                                                        >  1:49372/1‑139 (MQ=255)                             aCGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGTGGCAAAACTTg                                                                                      <  2:236458/139‑1 (MQ=255)                                  gtgtTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                 <  1:254363/139‑1 (MQ=255)                                         cAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGcc                                                                          <  2:49372/139‑1 (MQ=255)                                                 ccTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGt                                                                  >  2:700016/1‑139 (MQ=255)                                                     aTGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcgc                                                                                                                                  <  1:1589093/71‑1 (MQ=255)                                                     aTGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTcgc                                                                                                                                  >  2:1589093/1‑71 (MQ=255)                                                               cccGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGttt                                                    <  1:700016/139‑1 (MQ=255)                                                                   ttGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgcg                                                                                                   >  2:473209/1‑88 (MQ=255)                                                                   ttGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAgcg                                                                                                   <  1:473209/88‑1 (MQ=255)                                                                             gTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCgg                                      >  1:1894533/1‑139 (MQ=255)                                                                              tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAg                                                                                                     >  1:1831271/1‑75 (MQ=255)                                                                              tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAg                                                                                                     <  2:1831271/75‑1 (MQ=255)                                                                              tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                 >  2:778329/1‑95 (MQ=255)                                                                              tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                 <  1:778329/95‑1 (MQ=255)                                                                              tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTaa                                            >  1:2055959/1‑132 (MQ=255)                                                                              tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTaa                                            <  2:2055959/132‑1 (MQ=255)                                                                              tgatgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGa                                     >  1:920047/1‑139 (MQ=255)                                                                                atgaGCTGACAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAac                                   <  2:1894533/139‑1 (MQ=255)                                                                                        aCAGTCACTTTCGATGGGCATTCTTTGACAGCTcgcg                                                                                                                                 <  1:1601165/37‑1 (MQ=255)                                                                                        aCAGTCACTTTCGATGGGCATTCTTTGACAGCTcgcg                                                                                                                                 >  2:1601165/1‑37 (MQ=255)                                                                                        aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCggcggc                                                                                              <  1:886004/72‑1 (MQ=255)                                                                                        aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCggcggc                                                                                              >  2:886004/1‑72 (MQ=255)                                                                                        aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCtgtg                                                                            >  1:1239215/1‑90 (MQ=255)                                                                                        aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCtgtg                                                                            <  2:1239215/90‑1 (MQ=255)                                                                                        aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTg                                                         >  1:943321/1‑109 (MQ=255)                                                                                        aCAGTCACTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTg                                                         <  2:943321/109‑1 (MQ=255)                                                                                              aCTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                 >  1:714417/1‑79 (MQ=255)                                                                                              aCTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCg                                                                                 <  2:714417/79‑1 (MQ=255)                                                                                               cTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATg                    >  2:1793182/1‑139 (MQ=255)                                                                                               cTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATg                    >  2:228145/1‑139 (MQ=255)                                                                                               cTTTCGATGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATg                    <  2:920047/139‑1 (MQ=255)                                                                                                     aTGGGCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCtt              >  1:1125240/1‑139 (MQ=255)                                                                                                        ggCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCg                                       <  2:421767/111‑1 (MQ=255)                                                                                                        ggCATTCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCg                                       >  1:421767/1‑111 (MQ=255)                                                                                                            ttCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCa       <  2:1125240/139‑1 (MQ=255)                                                                                                            ttCTTTGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCa       <  1:778778/139‑1 (MQ=255)                                                                                                                 tGACAGCTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTa  <  1:1793182/139‑1 (MQ=255)                                                                                                                      |                                                                                                                                      AGCAAGTTTTTTACCCTCAAGGCCGGTGACGTTGTGTTGACAGGCACGCCTGATGGCGTCGGCCCGTTGCAAAGCGGTGATGAGCTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTA  >  NC_000913/1228607‑1228857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑