Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,661	T→C	M22V (ATG→GTG)	hofC ←	assembly protein in type IV pilin biogenesis, transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,661	0	T	C	100.0%	214.3 / NA	60	M22V (ATG→GTG)	hofC	assembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/31);  total (29/31)

TTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGG  >  NC_000913/115539‑115786                                                                                                                           |                                                                                                                              ttCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTg                                                                                                               <  2:802668/139‑1 (MQ=255)            tATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                    <  2:135576/139‑1 (MQ=255)            tATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                    <  1:2012900/139‑1 (MQ=255)                   tcGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgcca                                                                                             >  1:995247/1‑139 (MQ=255)                   tcGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgcca                                                                                             >  2:1435507/1‑139 (MQ=255)                     gCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatg                                                                                           >  2:1148405/1‑139 (MQ=255)                       cACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgcc                                                                                         <  2:1830296/139‑1 (MQ=255)                       cACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgcc                                                                                         <  2:995247/139‑1 (MQ=255)                            cgcAGAATTGATGGTGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTc                                                                                                          >  1:1014373/1‑117 (MQ=255)                            cgcAGAATTGATGGTGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTc                                                                                                          <  2:1014373/117‑1 (MQ=255)                                  aTTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGt                                                                              <  2:468876/139‑1 (MQ=255)                                      aTGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCt                                                                          >  1:969946/1‑139 (MQ=255)                                                gCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCAt                                                                <  2:1065709/139‑1 (MQ=255)                                                           aGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATcccc                                                     <  1:1435507/139‑1 (MQ=255)                                                            gTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgcc                                                                                              >  2:137447/1‑97 (MQ=255)                                                            gTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgcc                                                                                              <  1:137447/97‑1 (MQ=255)                                                                 gTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                               >  2:377310/1‑139 (MQ=255)                                                                 gTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                               >  1:971453/1‑139 (MQ=255)                                                                 gTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                               <  2:969946/139‑1 (MQ=255)                                                                  tAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAgcgc                                                                                    >  1:1432690/1‑101 (MQ=255)                                                                  tAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAgcgc                                                                                    <  2:1432690/101‑1 (MQ=255)                                                                         cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                       >  1:700279/1‑139 (MQ=255)                                                                          tGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                 <  1:569149/128‑1 (MQ=255)                                                                          tGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                 >  2:569149/1‑128 (MQ=255)                                                                          tGTTGCTGTAGTGCCATAAACAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAAc                                      >  2:1474020/1‑139 (MQ=255)                                                                                    gTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                         >  2:203863/1‑62 (MQ=255)                                                                                    gTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                         <  1:203863/62‑1 (MQ=255)                                                                                    gTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                          <  1:719390/77‑1 (MQ=255)                                                                                    gTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                          >  2:719390/1‑77 (MQ=255)                                                                                      gCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCg                                                             <  2:1947633/104‑1 (MQ=255)                                                                                      gCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCg                                                             >  1:1947633/1‑104 (MQ=255)                                                                                       ccATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgcc                                                                                         <  2:40632/75‑1 (MQ=255)                                                                                       ccATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgcc                                                                                         >  1:40632/1‑75 (MQ=255)                                                                                         aTAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                       <  1:1844627/139‑1 (MQ=255)                                                                                         aTAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                       <  1:377310/139‑1 (MQ=255)                                                                                            aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                          <  2:467653/69‑1 (MQ=255)                                                                                            aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                          >  1:467653/1‑69 (MQ=255)                                                                                            aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCGAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAg                    >  1:1199130/1‑139 (MQ=255)                                                                                            aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAg                    >  2:1061019/1‑139 (MQ=255)                                                                                              cAGTAAAGTACGGCTCTCTGCCCATAGCACCCCCTCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                             <  1:519547/128‑1 (MQ=255)                                                                                              cAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                    <  2:841510/57‑1 (MQ=255)                                                                                              cAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGt                                                                                                    >  1:841510/1‑57 (MQ=255)                                                                                              cAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATTCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                             >  2:519547/1‑128 (MQ=255)                                                                                                gTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCtt                <  2:700279/139‑1 (MQ=255)                                                                                                 tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                         >  1:1388724/1‑49 (MQ=255)                                                                                                 tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                         <  2:1388724/49‑1 (MQ=255)                                                                                                 tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCa                                                                 >  2:1668373/1‑89 (MQ=255)                                                                                                 tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCa                                                                 <  1:1668373/89‑1 (MQ=255)                                                                                                 tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                 >  2:655097/1‑105 (MQ=255)                                                                                                 tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTa                                                 <  1:655097/105‑1 (MQ=255)                                                                                                 tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCt                 >  1:1192778/1‑137 (MQ=255)                                                                                                 tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCt                 <  2:1192778/137‑1 (MQ=255)                                                                                                         ggCTCTCTGCCCATAGCACCCCATCTTGCGGATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGcc                                                                  <  1:1827889/80‑1 (MQ=255)                                                                                                         ggCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGcc                                                                  >  2:1827889/1‑80 (MQ=255)                                                                                                              tctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAgg  >  2:1520577/1‑139 (MQ=255)                                                                                                              tctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAgg  <  2:1124344/139‑1 (MQ=255)                                                                                                                tGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                               >  2:31132/1‑92 (MQ=255)                                                                                                                tGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                               <  1:31132/92‑1 (MQ=255)                                                                                                                     aTAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGt                                                                              <  2:51976/56‑1 (MQ=255)                                                                                                                     aTAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGt                                                                              >  1:51976/1‑56 (MQ=255)                                                                                                                           |                                                                                                                              TTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGG  >  NC_000913/115539‑115786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑