Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,505	C→T	S5N (AGC→AAC)  *322* (TAG→TAA)	sapC ← sapB ←	antimicrobial peptide transport ABC transporter permease antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,505	0	C	T	100.0%	216.8 / NA	66	S5N (AGC→AAC)  *322* (TAG→TAA)	sapC sapB	antimicrobial peptide transport ABC transporter permease antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/35);  total (31/35)

AAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGG  >  NC_000913/1354372‑1354623                                                                                                                                      |                                                                                                                       aaaCCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCACCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCg                                                                                                                   <  1:1806363/139‑1 (MQ=255)     cAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                               <  2:903262/139‑1 (MQ=255)                   acacAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTATGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCtt                                                                                                 <  2:995059/139‑1 (MQ=255)                     acaGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTAt                                                                                               >  2:1407261/1‑139 (MQ=255)                         tACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGttt                                                                                           >  2:328393/1‑139 (MQ=255)                           cagccagcCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         <  2:612603/139‑1 (MQ=255)                                   cccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCa                                                                                                            >  2:701079/1‑112 (MQ=255)                                   cccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCa                                                                                                            <  1:701079/112‑1 (MQ=255)                                               tACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTa                                                                                                                 <  1:1490366/95‑1 (MQ=255)                                               tACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTa                                                                                                                 >  2:1490366/1‑95 (MQ=255)                                               tACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCAccc                                                                     >  2:1409489/1‑139 (MQ=255)                                                 cAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCaa                                                                   <  1:341552/139‑1 (MQ=255)                                                      cGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                               >  2:641193/1‑90 (MQ=255)                                                      cGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                               <  1:641193/90‑1 (MQ=255)                                                             cGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTcc                                                                                                   >  2:355555/1‑95 (MQ=255)                                                             cGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTcc                                                                                                   <  1:355555/95‑1 (MQ=255)                                                                  agGCGTCACTATAAAATTTGTGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                               >  1:1357614/1‑78 (MQ=255)                                                                  agGCGTCACTATAAAATTTGTGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAg                                                                                                               <  2:1357614/78‑1 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTa                                                                                                                 <  2:1964171/71‑1 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTa                                                                                                                 >  1:1964171/1‑71 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCAt                                                                                                           >  2:353745/1‑77 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCAt                                                                                                           <  1:353745/77‑1 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCAt                                                                                                      <  2:1550233/82‑1 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCAt                                                                                                      >  1:1550233/1‑82 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         >  2:1079531/1‑95 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         <  1:1079531/95‑1 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACa                                             >  1:1942851/1‑139 (MQ=255)                                                                       tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTAGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGTCACCCAAAATATCAGAAATCACGTTAACa                                             >  2:1391111/1‑139 (MQ=255)                                                                          cTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCAtt                                                                                                     >  1:1001458/1‑80 (MQ=255)                                                                          cTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCAtt                                                                                                     <  2:1001458/80‑1 (MQ=255)                                                                              aaaaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         <  1:1391960/88‑1 (MQ=255)                                                                              aaaaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         >  2:1391960/1‑88 (MQ=255)                                                                               aaaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAc                                     <  1:328393/139‑1 (MQ=255)                                                                                 aTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCa                                   <  1:1407261/139‑1 (MQ=255)                                                                                   ttGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGt                                                                                   <  2:1196690/89‑1 (MQ=255)                                                                                   ttGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGt                                                                                   >  1:1196690/1‑89 (MQ=255)                                                                                     gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCg                                                                                                                   <  1:1747376/55‑1 (MQ=255)                                                                                     gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCg                                                                                                                   >  2:1747376/1‑55 (MQ=255)                                                                                     gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         >  1:1655175/1‑81 (MQ=255)                                                                                     gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         <  2:1655175/81‑1 (MQ=255)                                                                                                tACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGtt                                                 <  1:1585704/110‑1 (MQ=255)                                                                                                tACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGtt                                                 >  2:1585704/1‑110 (MQ=255)                                                                                                       cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATAcc                                                                                                        >  1:1861901/1‑48 (MQ=255)                                                                                                       cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATAcc                                                                                                        <  2:1861901/48‑1 (MQ=255)                                                                                                       cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         >  2:850445/1‑63 (MQ=255)                                                                                                       cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         <  1:850445/63‑1 (MQ=255)                                                                                                       cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAat                                                                >  2:1173677/1‑88 (MQ=255)                                                                                                       cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAat                                                                <  1:1173677/88‑1 (MQ=255)                                                                                                          gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCa                                                                        >  2:1980712/1‑77 (MQ=255)                                                                                                          gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCa                                                                        <  1:1980712/77‑1 (MQ=255)                                                                                                          gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTggacca                           >  2:1206996/1‑117 (MQ=255)                                                                                                          gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCca                           <  1:1206996/122‑1 (MQ=255)                                                                                                          gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcggc          >  2:1804979/1‑139 (MQ=255)                                                                                                                cgggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAt    <  1:1804979/139‑1 (MQ=255)                                                                                                                 gggcgCTTTTCGCTGTATACGTTATCGTAAGGCATAcc                                                                                                        >  1:333711/1‑38 (MQ=255)                                                                                                                 gggcgCTTTTCGCTGTATACGTTATCGTAAGGCATAcc                                                                                                        <  2:333711/38‑1 (MQ=255)                                                                                                                 gggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATg   >  2:1923226/1‑139 (MQ=255)                                                                                                                  ggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg  <  2:1942851/139‑1 (MQ=255)                                                                                                                  ggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg  <  1:1391111/139‑1 (MQ=255)                                                                                                                  ggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg  <  1:1923226/139‑1 (MQ=255)                                                                                                                    cgcTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         >  2:963512/1‑50 (MQ=255)                                                                                                                    cgcTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                         <  1:963512/50‑1 (MQ=255)                                                                                                                    cgcTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGc                             <  1:574789/110‑1 (MQ=255)                                                                                                                    cgcTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGc                             >  2:574789/1‑110 (MQ=255)                                                                                                                               gTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg  >  1:163732/1‑126 (MQ=255)                                                                                                                               gTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATgg  <  2:163732/126‑1 (MQ=255)                                                                                                                                      |                                                                                                                       AAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAATGG  >  NC_000913/1354372‑1354623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑