Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,159	G→A	*163* (TAG→TAA)  S51S (AGC→AGT)	sieB → ydaF ←	phage superinfection exclusion protein, Rac prophage uncharacterized protein, Rac prophage

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,159	0	G	A	100.0%	200.5 / NA	62	*163* (TAG→TAA)  S51S (AGC→AGT)	sieB ydaF	phage superinfection exclusion protein, Rac prophage uncharacterized protein, Rac prophage
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

AAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTG  >  NC_000913/1419025‑1419288                                                                                                                                       |                                                                                                                                  aaaaaaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTa                                                                                                                               >  1:250534/1‑139 (MQ=255)     aaTTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgt                                                                                                                           >  2:1545554/1‑139 (MQ=255)         aGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGc                                                                                                                       <  1:1899317/139‑1 (MQ=255)         aGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGc                                                                                                                       <  2:250534/139‑1 (MQ=255)           aaCGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCtt                                                                                                                     >  2:1972712/1‑139 (MQ=255)                 aGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCaa                                                                                                               >  2:1066794/1‑139 (MQ=255)                     catcatTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCAt                                                                                                           >  1:1365986/1‑139 (MQ=255)                          ttCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                      <  1:1545554/139‑1 (MQ=255)                               cATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTGATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCAc                                                                                                 >  1:240923/1‑139 (MQ=255)                                      gTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                          <  1:311705/139‑1 (MQ=255)                                      gTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                          <  1:1972712/139‑1 (MQ=255)                                      gTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTc                                                                                          <  1:138693/139‑1 (MQ=255)                                         aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                        >  1:1740270/1‑106 (MQ=255)                                         aTAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                        <  2:1740270/106‑1 (MQ=255)                                          tAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGtt                                                                                      >  1:1685295/1‑139 (MQ=255)                                            gcgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATAGTTAACTTACTATGTGCCTCTCCAACCATCGGCGCGCACCAGTTTCGGtttt                                                                                    >  2:1361262/1‑139 (MQ=255)                                             cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTCTTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGATTTTa                                                                                   >  1:1122452/1‑139 (MQ=255)                                             cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACt                                                                                                                             <  2:817537/97‑1 (MQ=255)                                             cgcAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACt                                                                                                                             >  1:817537/1‑97 (MQ=255)                                                       tCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCtt                                                                                                                     >  2:1021327/1‑95 (MQ=255)                                                       tCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCtt                                                                                                                     <  1:1021327/95‑1 (MQ=255)                                                       tCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCAt                                                                                                           <  1:4878/105‑1 (MQ=255)                                                       tCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCAt                                                                                                           >  2:4878/1‑105 (MQ=255)                                                        cTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCt                                                                        <  2:1365986/139‑1 (MQ=255)                                                           attatCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCtttt                                                                     >  2:2030106/1‑139 (MQ=255)                                                                ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCtt                                                                                                                     <  1:1340627/86‑1 (MQ=255)                                                                ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCtt                                                                                                                     >  2:1340627/1‑86 (MQ=255)                                                                ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtat                                                                >  1:1896152/1‑139 (MQ=255)                                                                ctcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtat                                                                >  1:1714649/1‑139 (MQ=255)                                                                 tcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTGATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtata                                                               <  2:240923/139‑1 (MQ=255)                                                                 tcGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGtata                                                               <  1:1066794/139‑1 (MQ=255)                                                                      aCCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCa                                                          <  2:368255/139‑1 (MQ=255)                                                                                  tCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                 >  2:1941038/1‑120 (MQ=255)                                                                                  tCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGta                                                                 <  1:1941038/120‑1 (MQ=255)                                                                                    ccATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGtt                                            >  2:1923928/1‑139 (MQ=255)                                                                                    ccATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGtt                                            <  2:1685295/139‑1 (MQ=255)                                                                                        tttATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCAt                                        >  1:825550/1‑139 (MQ=255)                                                                                        tttATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCAt                                        <  1:1923928/139‑1 (MQ=255)                                                                                        tttATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCAt                                        <  2:825550/139‑1 (MQ=255)                                                                                            tGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCTTCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTg                                    >  2:399/1‑139 (MQ=255)                                                                                                    ttCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                        <  1:1419327/47‑1 (MQ=255)                                                                                                    ttCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                        >  2:1419327/1‑47 (MQ=255)                                                                                                         gAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                        >  1:802726/1‑42 (MQ=255)                                                                                                         gAACAGCAGGAGTAGACGTTTTAATCGTTAACTTACtgtgtg                                                                                                                        <  2:802726/42‑1 (MQ=255)                                                                                                            cagcagGAGTAGACGTTTTAATCGTTAACTTACTATGTGCCTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                    <  1:1361262/139‑1 (MQ=255)                                                                                                               cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                      >  2:1731578/1‑54 (MQ=255)                                                                                                               cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGgcg                                                                                                      <  1:1731578/54‑1 (MQ=255)                                                                                                               cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCtt                                                                       <  2:897251/85‑1 (MQ=255)                                                                                                               cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCtt                                                                       >  1:897251/1‑85 (MQ=255)                                                                                                               cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTg                                                                    >  2:250810/1‑88 (MQ=255)                                                                                                               cagGAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTg                                                                    <  1:250810/88‑1 (MQ=255)                                                                                                                 ggAGTAGACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCTTCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCacac               <  1:399/139‑1 (MQ=255)                                                                                                                       gACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGa                                                 <  1:775946/99‑1 (MQ=255)                                                                                                                       gACGTTTTAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGa                                                 >  2:775946/1‑99 (MQ=255)                                                                                                                            tttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCacac               >  2:1342803/1‑128 (MQ=255)                                                                                                                            tttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCacac               <  1:1342803/128‑1 (MQ=255)                                                                                                                             ttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGttt                                                                            <  2:124603/66‑1 (MQ=255)                                                                                                                             ttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGttt                                                                            >  1:124603/1‑66 (MQ=255)                                                                                                                             ttAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgtt   >  1:1138990/1‑139 (MQ=255)                                                                                                                              tAATCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCgttg  <  2:1714649/139‑1 (MQ=255)                                                                                                                               aaTCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                    >  2:1056195/1‑120 (MQ=255)                                                                                                                               aaTCGTTAACTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACAcgccg                    <  1:1056195/120‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  AAAAAATTAGAACGGAAGGGCATCATTCAACATCAGAGTGATAGCGCAAACTGTTCTTATTATCTCGTCACCGAAAAATACTCCCATTTTATGAAGTTATTCTGGAACAGCAGGAGTAGACGTTTTAATCGTTAGCTTACTGTGTGCTTCTCCAACCATCGGCGCGCACCAGTTTCGGTTTTAAATGTTTTGCTTTTGGTATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTG  >  NC_000913/1419025‑1419288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑