Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,431,882	T→C	V945A (GTG→GCG)	stfR →	Rac prophage, putative tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,431,882	0	T	C	100.0%	211.9 / NA	59	V945A (GTG→GCG)	stfR	Rac prophage, putative tail fiber protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/32);  total (27/32)

CACCAGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGTGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAA  >  NC_000913/1431747‑1432016                                                                                                                                        |                                                                                                                                       caccaGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGAt                                                                                                                                     <  2:1629609/139‑1 (MQ=255)  accaGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATc                                                                                                                                    <  2:821363/139‑1 (MQ=255)  accaGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATc                                                                                                                                    <  1:1662084/139‑1 (MQ=255)                 aGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGc                                                                                                                     >  2:271378/1‑139 (MQ=255)                  gTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCt                                                                                                                    <  1:344659/139‑1 (MQ=255)                     ggCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGa                                                                                                                 <  1:1124805/139‑1 (MQ=255)                          aCCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAtt                                                                                                            <  1:732566/139‑1 (MQ=255)                                     ggCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATc                                                                                                                                    >  1:493430/1‑104 (MQ=255)                                     ggCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATc                                                                                                                                    <  2:493430/104‑1 (MQ=255)                                          tCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCa                                                                                            <  1:271378/139‑1 (MQ=255)                                                aCCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTc                                                                                      >  1:10818/1‑139 (MQ=255)                                                   gTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCggg                                                                                   <  2:1322257/139‑1 (MQ=255)                                                     tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc                                                                                                      <  2:42497/118‑1 (MQ=255)                                                     tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGc                                                                                                      >  1:42497/1‑118 (MQ=255)                                                     tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGcc                                                                                 >  2:1436159/1‑139 (MQ=255)                                                     tCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGcc                                                                                 >  2:404168/1‑139 (MQ=255)                                                       cGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGt                                                                               >  1:750239/1‑139 (MQ=255)                                                        gTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                              <  1:1626941/139‑1 (MQ=255)                                                        gTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                              <  1:404168/139‑1 (MQ=255)                                                        gTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                              <  1:1436159/139‑1 (MQ=255)                                                                       gATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGt                                                                                       <  1:1774437/115‑1 (MQ=255)                                                                       gATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGt                                                                                       >  2:1774437/1‑115 (MQ=255)                                                                         tGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCCGCCGCTTATCCGTCAGGCGCGATCCCTGATACGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGa                                                             <  1:1339147/139‑1 (MQ=255)                                                                         tGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGa                                                             <  2:750239/139‑1 (MQ=255)                                                                           cAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                              <  2:595225/120‑1 (MQ=255)                                                                           cAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTa                                                                              >  1:595225/1‑120 (MQ=255)                                                                              gggCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCa                                                        >  1:1822943/1‑139 (MQ=255)                                                                                  aGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaa                                                    >  2:2020310/1‑139 (MQ=255)                                                                                   ggCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAtt                                                                                                            >  1:1527200/1‑82 (MQ=255)                                                                                   ggCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGAtt                                                                                                            <  2:1527200/82‑1 (MQ=255)                                                                                   ggCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTaaa                                                   >  2:2020214/1‑139 (MQ=255)                                                                                       tttGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGc                                               >  1:1150718/1‑139 (MQ=255)                                                                                              aaTCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCca                                        >  2:1660857/1‑139 (MQ=255)                                                                                               aTCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGa                                                             >  2:1024056/1‑117 (MQ=255)                                                                                               aTCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGa                                                             <  1:1024056/117‑1 (MQ=255)                                                                                                tCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCcaca                                      >  1:576772/1‑139 (MQ=255)                                                                                                   gCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGa                                                             >  2:1076876/1‑113 (MQ=255)                                                                                                   gCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGa                                                             <  1:1076876/113‑1 (MQ=255)                                                                                                       aCCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGAc                                                            >  1:1049676/1‑110 (MQ=255)                                                                                                       aCCCGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGAc                                                            <  2:1049676/110‑1 (MQ=255)                                                                                                        cccGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCAcagcgccagc                              <  2:10818/139‑1 (MQ=255)                                                                                                        cccGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCAcagcgccagc                              <  1:2020214/139‑1 (MQ=255)                                                                                                        cccGAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGACCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCAcagcgccagc                              <  2:23294/139‑1 (MQ=255)                                                                                                           gAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCa                           <  2:1470738/139‑1 (MQ=255)                                                                                                           gAAACTTGCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCa                           <  2:1150718/139‑1 (MQ=255)                                                                                                                  gCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGtct                                                                        >  2:918028/1‑87 (MQ=255)                                                                                                                  gCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGtct                                                                        <  1:918028/87‑1 (MQ=255)                                                                                                                  gCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAAt                                                  <  2:340046/109‑1 (MQ=255)                                                                                                                  gCAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAAt                                                  >  1:340046/1‑109 (MQ=255)                                                                                                                   cAGCCGCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCACACCCACAGCGCCAGCGCATCCAGTAc                   >  1:1310012/1‑139 (MQ=255)                                                                                                                        gCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCa                           <  1:668250/126‑1 (MQ=255)                                                                                                                        gCTTATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCa                           >  2:668250/1‑126 (MQ=255)                                                                                                                          ttATCCGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTg            <  1:1660857/139‑1 (MQ=255)                                                                                                                               cGTCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGAc       <  2:370783/139‑1 (MQ=255)                                                                                                                                 tCAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGa     >  1:2056428/1‑139 (MQ=255)                                                                                                                                  cAGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGaa    <  2:576772/139‑1 (MQ=255)                                                                                                                                   aGGCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGaaa   >  2:708155/1‑139 (MQ=255)                                                                                                                                    ggCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGaaaa  >  2:1138297/1‑139 (MQ=255)                                                                                                                                    ggCGCGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGaaaa  >  1:1224109/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                       CACCAGAAAGCTACCCAGTCGGCGCACCAATCCCGTGGCCATCAGATACCGTTCCGTCTGGTTATGCCCTGATGCAGGGGCAGGCTTTTGACAAATCTGCTTACCCGAAACTTGCAGCCGCTTATCCGTCAGGCGTGATCCCTGATATGCGTGGCTGGACGATTAAGGGCAAACCTGCCAGTGGTCGGGCCGTATTGTCTCAGGAACAGGACGGCATTAAATCGCATACCCACAGCGCCAGCGCATCCAGTACAGATTTGGGGACGAAAA  >  NC_000913/1431747‑1432016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑