Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,525,144	C→A	L279F (TTG→TTT)	ansP ←	L‑asparagine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,525,144	0	C	A	100.0%	279.0 / NA	85	L279F (TTG→TTT)	ansP	L‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (46/39);  total (46/39)

ACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAAT  >  NC_000913/1525015‑1525275                                                                                                                                  |                                                                                                                                    aCCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata                                                                                                                            <  1:893499/139‑1 (MQ=255)   cTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCC‑GTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAAcc                                                                                                                         >  2:124830/1‑139 (MQ=255)      aaTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAg                                                                                                                       >  2:1315544/1‑139 (MQ=255)      aaTTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAg                                                                                                                       >  2:526561/1‑139 (MQ=255)             gCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAAcacc                                                                                                                >  2:79434/1‑139 (MQ=255)                   gagGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCC‑GTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                         <  1:124830/139‑1 (MQ=255)                    agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                         <  1:526561/139‑1 (MQ=255)                    agGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                         <  1:1315544/139‑1 (MQ=255)                                aGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACa                                                                                             >  1:335628/1‑139 (MQ=255)                                      acaATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCaa                                                                                       <  1:79434/139‑1 (MQ=255)                                            ttCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGcc                                                                                 >  2:1215476/1‑139 (MQ=255)                                               aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataac                                                                                                                               <  2:15082/90‑1 (MQ=255)                                               aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataac                                                                                                                               >  1:15082/1‑90 (MQ=255)                                               aTAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                                                              >  2:1888411/1‑139 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACa                                                                                             >  1:718886/1‑117 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACa                                                                                             <  2:718886/117‑1 (MQ=255)                                                      tgcCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACt                                                                       >  1:1100596/1‑139 (MQ=255)                                                        ccGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataa                                                                                                                                >  2:1145620/1‑80 (MQ=255)                                                        ccGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataa                                                                                                                                <  1:1145620/80‑1 (MQ=255)                                                        ccGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                         <  2:1935625/103‑1 (MQ=255)                                                        ccGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                         >  1:1935625/1‑103 (MQ=255)                                                        ccGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGt                                                                     >  2:1017969/1‑139 (MQ=255)                                                             atatGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATg                                                                >  1:694400/1‑139 (MQ=255)                                                                  gCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCttt                                                           >  2:1209687/1‑139 (MQ=255)                                                                    acacCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                                                         <  2:335628/139‑1 (MQ=255)                                                                    acacCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAg                                                         <  1:918113/139‑1 (MQ=255)                                                                     cacCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                         >  1:1689320/1‑90 (MQ=255)                                                                     cacCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGc                                                                                                         <  2:1689320/90‑1 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataac                                                                                                                               <  2:1870419/67‑1 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataac                                                                                                                               >  1:1870419/1‑67 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAAcacca                                                                                                               <  1:1317427/83‑1 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAAcacca                                                                                                               >  2:1317427/1‑83 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca                                                                         <  2:1438081/121‑1 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATcaca                                                                         >  1:1438081/1‑121 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                                                       >  1:1588401/1‑139 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                                                       >  2:1764378/1‑139 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGc                                                       >  1:290493/1‑139 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTACGc                                                       >  1:1367494/1‑139 (MQ=255)                                                                      acCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCCTCAGGc                                                       >  1:841813/1‑139 (MQ=255)                                                                       cccAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata                                                                                                                            >  2:461074/1‑69 (MQ=255)                                                                       cccAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacata                                                                                                                            <  1:461074/69‑1 (MQ=255)                                                                        ccAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAc                                                     >  2:886294/1‑139 (MQ=255)                                                                        ccAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCAc                                                     >  1:517455/1‑139 (MQ=255)                                                                            tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATa                                                                                     <  1:1608681/103‑1 (MQ=255)                                                                            tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATa                                                                                     >  2:1608681/1‑103 (MQ=255)                                                                            tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATca                                                                           <  2:653469/113‑1 (MQ=255)                                                                            tttAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATca                                                                           >  1:653469/1‑113 (MQ=255)                                                                                gagaAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCaa                                                                                       >  1:1597852/1‑97 (MQ=255)                                                                                gagaAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCaa                                                                                       <  2:1597852/97‑1 (MQ=255)                                                                                   aaaaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg                                          <  2:694400/139‑1 (MQ=255)                                                                                     aaaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGa                                        >  2:721853/1‑139 (MQ=255)                                                                                      aaCGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGAt                                       >  1:1898891/1‑139 (MQ=255)                                                                                        cGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacataac                                                                                                                          >  1:290097/1‑54 (MQ=255)                                                                                        cGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAataacataac                                                                                                                          <  2:290097/54‑1 (MQ=255)                                                                                               aaCGGACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACAtt                              <  1:1209687/139‑1 (MQ=255)                                                                                                  ggACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCaa                                                                                       >  1:693584/1‑79 (MQ=255)                                                                                                  ggACTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCaa                                                                                       <  2:693584/79‑1 (MQ=255)                                                                                                     cTTTGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCgg                        <  2:517455/139‑1 (MQ=255)                                                                                                        tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACg                                                                                   >  1:263274/1‑77 (MQ=255)                                                                                                        tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACg                                                                                   <  2:263274/77‑1 (MQ=255)                                                                                                        tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg                                          >  1:1832160/1‑118 (MQ=255)                                                                                                        tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCg                                          <  2:1832160/118‑1 (MQ=255)                                                                                                        tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAg                     >  1:356524/1‑139 (MQ=255)                                                                                                        tGCCCCGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAg                     <  2:1100596/139‑1 (MQ=255)                                                                                                             cGCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTa                <  2:356524/139‑1 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACg                                                                                                    <  1:598672/54‑1 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACg                                                                                                    >  2:598672/1‑54 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCa                                                                                <  2:971761/74‑1 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCa                                                                                >  1:971761/1‑74 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCt                                                             <  2:1338913/93‑1 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTAc               >  2:1927153/1‑139 (MQ=255)                                                                                                              gCCTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGACAATACGCCAAATCACACTGTTAATGGCt                                                             >  1:1338913/1‑93 (MQ=255)                                                                                                               ccTGATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGCTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTAcc              <  1:1888411/139‑1 (MQ=255)                                                                                                                  gATACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCGAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACccac           <  1:886294/139‑1 (MQ=255)                                                                                                                   aTACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTaaaa                                                                                               <  1:774022/54‑1 (MQ=255)                                                                                                                   aTACGCGCTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTaaaa                                                                                               >  2:774022/1‑54 (MQ=255)                                                                                                                         gcTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                                                              >  2:415469/1‑65 (MQ=255)                                                                                                                         gcTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCaaa                                                                              <  1:415469/65‑1 (MQ=255)                                                                                                                          cTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACg                                                                                                    >  2:58929/1‑42 (MQ=255)                                                                                                                          cTCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACg                                                                                                    <  1:58929/42‑1 (MQ=255)                                                                                                                           tCCACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAAt  <  2:290493/139‑1 (MQ=255)                                                                                                                             cACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTaaaa                                                                                               >  2:1377583/1‑44 (MQ=255)                                                                                                                             cACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTaaaa                                                                                               <  1:1377583/44‑1 (MQ=255)                                                                                                                             cACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCgg                        <  2:1589339/115‑1 (MQ=255)                                                                                                                             cACGGAAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCgg                        >  1:1589339/1‑115 (MQ=255)                                                                                                                                  |                                                                                                                                    ACCTGAATTCAGGCTGGAGAGGGCAGCGGTCAGCACCACAATGTTCATAATGCTGCCGATATATGGCACACCCAGTTTAGAGAAAAACGTCACGAACGGACTTTGCCCCGCCTGATACGCGCTCCACGGCAATAACATAACCAGCAACACCACGGAGCCGACGTAAAACAGGCCAATACGCCAAATCACACTGTTAATGGCTTTAGGCACCATGGTCTGCGGATCTTTACATTCTCCGGCAGCTGTACCCACCATTTCAAT  >  NC_000913/1525015‑1525275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑