Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	162,927	G→A	A275T (GCT→ACT)	hrpB →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	162,927	0	G	A	100.0%	220.2 / NA	68	A275T (GCT→ACT)	hrpB	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/34);  total (34/34)

CAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAC  >  NC_000913/162795‑163057                                                                                                                                     |                                                                                                                                   caacTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaaa                                                                                                                              <  1:224065/139‑1 (MQ=255)   acTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAcc                                                                                                                            >  1:931203/1‑139 (MQ=255)   acTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAcc                                                                                                                            >  1:297613/1‑139 (MQ=255)   acTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAcc                                                                                                                            >  1:2044859/1‑139 (MQ=255)    cTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAc                                                                                                                             >  2:1919109/1‑137 (MQ=255)    cTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAAc                                                                                                                             <  1:1919109/137‑1 (MQ=255)        cTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGttt                                                                                                                       >  2:1679949/1‑139 (MQ=255)             cgcATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCa                                                                                                                  <  1:1823633/139‑1 (MQ=255)                            gTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                                   <  2:297613/139‑1 (MQ=255)                            gTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                                   <  2:2044859/139‑1 (MQ=255)                                  ctctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGa                                                                                                              <  1:1072097/122‑1 (MQ=255)                                  ctctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGa                                                                                                              >  2:1072097/1‑122 (MQ=255)                                  ctctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtgg                                                                                             >  1:538048/1‑139 (MQ=255)                                   tctGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggt                                                                                            <  2:713713/139‑1 (MQ=255)                                     tGCCCGCTGTATGTCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGttt                                                                                                                       >  2:1418751/1‑110 (MQ=255)                                     tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACt                                                                                                                                  <  2:35533/99‑1 (MQ=255)                                     tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGttt                                                                                                                       <  1:1418751/110‑1 (MQ=255)                                     tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTAt                                                                                                        <  1:1720280/125‑1 (MQ=255)                                     tGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTAt                                                                                                        >  2:1720280/1‑125 (MQ=255)                                      gCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGa                                                                                         <  2:931203/139‑1 (MQ=255)                                      gCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCG‑AAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCAtt                                                                                                                >  2:442294/1‑115 (MQ=255)                                      gCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCG‑AAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCAtt                                                                                                                <  1:442294/115‑1 (MQ=255)                                       cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAt                                                                                        >  2:1822520/1‑139 (MQ=255)                                       cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAt                                                                                        >  2:436252/1‑139 (MQ=255)                                       cccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTACCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggtggct                                                                                        >  1:1762927/1‑137 (MQ=255)                                        ccGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAtt                                                                                       <  1:436252/139‑1 (MQ=255)                                                 ggCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                                   <  1:224717/118‑1 (MQ=255)                                                 ggCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTc                                                                                                   >  2:224717/1‑118 (MQ=255)                                                 ggCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAgg                                                                              >  2:1080593/1‑139 (MQ=255)                                                     cgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCATGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCg                                                                          >  1:1304024/1‑139 (MQ=255)                                                     cgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCa                                                                                >  1:221588/1‑133 (MQ=255)                                                     cgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCa                                                                                <  2:221588/133‑1 (MQ=255)                                                     cgTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCg                                                                          >  2:592221/1‑139 (MQ=255)                                                        tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTTAAACCAGt                                                                                                                         >  2:154013/1‑89 (MQ=255)                                                        tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGt                                                                                                                         <  1:154013/89‑1 (MQ=255)                                                        tGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCgtgt                                                                       >  2:107966/1‑139 (MQ=255)                                                          tCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTgg                                                                     >  1:1169333/1‑139 (MQ=255)                                                           cGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgc                                                                    <  1:107966/139‑1 (MQ=255)                                                             cTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGgcgc                                                                  >  2:1786366/1‑139 (MQ=255)                                                                aaCGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTa                                                                                                                      <  1:1067131/84‑1 (MQ=255)                                                                aaCGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTa                                                                                                                      >  2:1067131/1‑84 (MQ=255)                                                                      cAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATc                                                         <  1:1786366/139‑1 (MQ=255)                                                                        gCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                       <  1:1679949/139‑1 (MQ=255)                                                                          gAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                     >  1:1493221/1‑139 (MQ=255)                                                                            aaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                   <  1:592221/139‑1 (MQ=255)                                                                                 gATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGt                                                                                                    >  2:1769604/1‑85 (MQ=255)                                                                                 gATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGt                                                                                                    <  1:1769604/85‑1 (MQ=255)                                                                                       cccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcg                                        <  1:1080593/139‑1 (MQ=255)                                                                                         cGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                      <  2:1493221/139‑1 (MQ=255)                                                                                         cGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                      <  1:290051/139‑1 (MQ=255)                                                                                         cGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcgcg                                      <  2:583144/139‑1 (MQ=255)                                                                                          ggCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGa                                     >  2:914902/1‑139 (MQ=255)                                                                                                 cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaa                                                                                                                               <  2:1470763/42‑1 (MQ=255)                                                                                                 cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGaa                                                                                                                               >  1:1470763/1‑42 (MQ=255)                                                                                                 cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCa                                                                                <  1:1719793/89‑1 (MQ=255)                                                                                                 cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCa                                                                                >  2:1719793/1‑89 (MQ=255)                                                                                                 cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTa                              >  2:293480/1‑139 (MQ=255)                                                                                                    gggATGCGCAAAGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                           >  2:358573/1‑139 (MQ=255)                                                                                                               aGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTgg                                                                     <  1:630049/86‑1 (MQ=255)                                                                                                               aGTGGTGCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTgg                                                                     >  2:630049/1‑86 (MQ=255)                                                                                                                gtggtgCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTa                                                                                                         <  1:65891/49‑1 (MQ=255)                                                                                                                gtggtgCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTa                                                                                                         >  2:65891/1‑49 (MQ=255)                                                                                                                 tggtgCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCa              <  1:358573/139‑1 (MQ=255)                                                                                                                    tgCTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGc           <  2:1304024/139‑1 (MQ=255)                                                                                                                      cTGGCGACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCAt         <  2:538048/139‑1 (MQ=255)                                                                                                                           gACCAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATg    >  2:1459787/1‑139 (MQ=255)                                                                                                                              cAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc  >  1:1661506/1‑138 (MQ=255)                                                                                                                              cAATATTACTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc  <  2:1661506/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   CAACTGGCTTCGCGCATCGGCAGTGATGTATTGCTCTGCCCGCTGTATGGCGCGTTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAC  >  NC_000913/162795‑163057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑