Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,794,172	C→T	*327* (TAG→TAA)	btuC ←	vitamin B12 ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,794,172	0	C	T	100.0%	222.5 / NA	69	*327* (TAG→TAA)	btuC	vitamin B12 ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/32);  total (37/32)

GAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGC  >  NC_000913/1794041‑1794299                                                                                                                                    |                                                                                                                                gAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTc                                                                                                                          <  1:681423/139‑1 (MQ=255)        tcCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCttt                                                                                                                   <  2:161546/139‑1 (MQ=255)          cAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTa                                                                                                                 >  2:1779281/1‑139 (MQ=255)             cGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACa                                                                                                              >  1:47047/1‑139 (MQ=255)                ggTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTTGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTGGCGGTTAACGTCCTGCTTTTAACAATa                                                                                                           <  1:869726/139‑1 (MQ=255)                ggTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATa                                                                                                           <  2:563998/139‑1 (MQ=255)                 gTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATaa                                                                                                          >  1:1002252/1‑139 (MQ=255)                           cGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCtttt                                                                                                                  >  1:635235/1‑121 (MQ=255)                           cGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCtttt                                                                                                                  <  2:635235/121‑1 (MQ=255)                           cGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAac                                                                                                >  2:1549215/1‑139 (MQ=255)                                atatCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACa                                                                                                              >  2:1215793/1‑120 (MQ=255)                                atatCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACa                                                                                                              <  1:1215793/120‑1 (MQ=255)                                     tttCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCAc                                                                                      >  1:924937/1‑139 (MQ=255)                                      ttCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCAcc                                                                                     <  2:1002252/139‑1 (MQ=255)                                         aCTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTaa                                                                                  >  2:1054844/1‑139 (MQ=255)                                               gtcgtcAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGC‑TTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATa                                                                                                           <  2:1266723/107‑1 (MQ=255)                                               gtcgtcAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGC‑TTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATa                                                                                                           >  1:1266723/1‑107 (MQ=255)                                                     aGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGAc                                                                      >  1:134621/1‑139 (MQ=255)                                                       aaTGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                    <  1:1779281/139‑1 (MQ=255)                                                       aaTGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                    >  1:1818972/1‑139 (MQ=255)                                                       aaTGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCAGCTTTTGACAATAACCAGATAACCACCGGCGCACCTAACGTTGCGGTGACCa                                                                    <  1:1189176/139‑1 (MQ=255)                                                                                 ggTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCt                                          <  1:1549215/139‑1 (MQ=255)                                                                                       gTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGcc                                    >  2:428779/1‑139 (MQ=255)                                                                                            tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                               <  2:47047/139‑1 (MQ=255)                                                                                            tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                               <  2:120891/139‑1 (MQ=255)                                                                                            tAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                               <  2:1818972/139‑1 (MQ=255)                                                                                                    ttAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCgg                                                                                           <  1:1678697/71‑1 (MQ=255)                                                                                                    ttAGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCgg                                                                                           >  2:1678697/1‑71 (MQ=255)                                                                                                      aGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAaca                                                                                               <  2:1612793/65‑1 (MQ=255)                                                                                                      aGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAaca                                                                                               >  1:1612793/1‑65 (MQ=255)                                                                                                      aGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                               <  2:955292/129‑1 (MQ=255)                                                                                                      aGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                               >  1:955292/1‑129 (MQ=255)                                                                                                      aGCTTTTAATCATAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAata                     <  2:924937/139‑1 (MQ=255)                                                                                                                  tAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                               >  2:1634158/1‑117 (MQ=255)                                                                                                                  tAGACCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGgcg                               <  1:1634158/117‑1 (MQ=255)                                                                                                                     aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCg                                                                                            <  1:1084903/53‑1 (MQ=255)                                                                                                                     aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCg                                                                                            >  2:1084903/1‑53 (MQ=255)                                                                                                                     aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCa                                                               <  1:439915/82‑1 (MQ=255)                                                                                                                     aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCa                                                               >  2:439915/1‑82 (MQ=255)                                                                                                                     aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAAt      >  2:1825701/1‑139 (MQ=255)                                                                                                                     aCCGTCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAAt      >  2:1127542/1‑139 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAAcc                                                                                                        >  2:1968031/1‑37 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAAcc                                                                                                        <  1:1968031/37‑1 (MQ=38)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCTGATAAACACCGGCGCACCTAACGTTGCTGTGa                                                                       >  2:1016493/1‑70 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAaca                                                                                               <  1:1585183/46‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAaca                                                                                               >  2:1585183/1‑46 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCa                                                                                       <  1:1472366/54‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCa                                                                                       >  2:1472366/1‑54 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCg                                                                           >  1:950374/1‑66 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCg                                                                           <  2:950374/66‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGa                                                                       <  1:1016493/70‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                    <  1:85765/73‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                    <  2:26779/73‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                    >  1:26779/1‑73 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCa                                                                    >  2:85765/1‑73 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCgg                                               <  1:641118/94‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCgg                                               >  2:641118/1‑94 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAgg                                 >  2:1075560/1‑108 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAgg                                 >  1:694292/1‑108 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAgg                                 <  1:1075560/108‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAgg                                 <  2:694292/108‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  2:96912/1‑139 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  2:1568729/1‑139 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  1:1110203/1‑139 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  <  1:1127542/139‑1 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  2:905486/1‑139 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  1:1863930/1‑139 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  1:1938811/1‑139 (MQ=255)                                                                                                                         tCTTTTTGCGGTTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATgcgc  >  1:258110/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                GAACTTCTCCAGCGTGGTCACTTCACCGTCGATATCTTTCACTACGGTCGTCAGAATGGAATCTTGCATCGTTTCTCCTGGGTGTGGTCAGTAAAAATCTTAGCTTTTAATCATAGACCGTCTTTTTGCGGCTAACGTCCTGCTTTTAACAATAACCAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGC  >  NC_000913/1794041‑1794299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑