Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,325	A→G	T104T (ACT→ACC)	yeaE ←	aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,325	0	A	G	100.0%	181.9 / NA	53	T104T (ACT→ACC)	yeaE	aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/23);  total (30/23)

AGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCA  >  NC_000913/1865211‑1865460                                                                                                                   |                                                                                                                                        aGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGc                                                                                                                  >  1:361346/1‑138 (MQ=255) aGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGc                                                                                                                  <  2:361346/138‑1 (MQ=255) aGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGT‑AAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCtt                                                                                                                >  2:554894/1‑139 (MQ=255)   aaaCGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTc                                                                                                               >  2:1564143/1‑139 (MQ=255)   aaaCGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTc                                                                                                               >  1:914078/1‑139 (MQ=255)        ccccAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCtt                                                                                                                >  2:1207935/1‑133 (MQ=255)        ccccAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCtt                                                                                                                <  1:1207935/133‑1 (MQ=255)         cccAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACAAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGc                                                                                                                  <  2:1421754/130‑1 (MQ=255)         cccAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACAAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGc                                                                                                                  >  1:1421754/1‑130 (MQ=255)                   gATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCt                                                                                               >  1:1260956/1‑139 (MQ=255)                              gggCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGcc                                                                                    >  2:1129865/1‑139 (MQ=255)                                   aTCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCAt                                                                               >  1:600159/1‑139 (MQ=255)                                    tCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCAtt                                                                              >  2:1459712/1‑139 (MQ=255)                                     cAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTc                                                                             <  2:1260956/139‑1 (MQ=255)                                          tttCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACgg                                                                        <  1:1564143/139‑1 (MQ=255)                                                        aCAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAgaga                                                          <  1:1459712/139‑1 (MQ=255)                                                           gTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGcc                                                                                        >  2:1046345/1‑106 (MQ=255)                                                           gTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGcc                                                                                        <  1:1046345/106‑1 (MQ=255)                                                            tctcTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTcc                                                                            <  2:1807844/117‑1 (MQ=255)                                                            tctcTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTcc                                                                            >  1:1807844/1‑117 (MQ=255)                                                                      aGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgaga                                                                                                                                >  1:296940/1‑55 (MQ=255)                                                                      aGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTgaga                                                                                                                                <  2:296940/55‑1 (MQ=255)                                                                          aaaCTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                     <  1:34915/110‑1 (MQ=255)                                                                          aaaCTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                     >  2:34915/1‑110 (MQ=255)                                                                               gCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGa                                   <  2:600159/139‑1 (MQ=255)                                                                                ccAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGAc                                  <  1:1129865/139‑1 (MQ=255)                                                                                 cAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGcc                                                                                        <  1:1837048/84‑1 (MQ=255)                                                                                 cAGACCAGTGTAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGcc                                                                                        >  2:1837048/1‑84 (MQ=255)                                                                                        gtgtAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAAt                          >  1:140332/1‑139 (MQ=255)                                                                                        gtgtAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAAt                          >  2:1928271/1‑139 (MQ=255)                                                                                          gtAATAAGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGc                        >  1:864455/1‑139 (MQ=255)                                                                                                aGTAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTcccc                  >  2:499694/1‑139 (MQ=255)                                                                                                  tAAAGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCaa                >  1:200135/1‑139 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCtttt                                                                                            <  2:115553/62‑1 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCtttt                                                                                            >  1:115553/1‑62 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGa                                                                       <  2:1106462/83‑1 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGa                                                                       >  1:1106462/1‑83 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGAt                                                                      >  2:1153057/1‑84 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGAt                                                                      <  1:1153057/84‑1 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGAt                                                                      <  1:1183183/84‑1 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGAt                                                                      >  2:1183183/1‑84 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACg                                                        >  2:988005/1‑98 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACg                                                        <  1:988005/98‑1 (MQ=255)                                                                                                   aaaGATCGAGATAATCGGTATTGAGACAGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAac               >  2:1331907/1‑139 (MQ=255)                                                                                                    aaGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAacc              <  2:864455/139‑1 (MQ=255)                                                                                                     aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAg                                                                                  >  2:161061/1‑70 (MQ=255)                                                                                                     aGATCGAGATAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAg                                                                                  <  1:161061/70‑1 (MQ=255)                                                                                                           agaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                     <  2:1080043/77‑1 (MQ=255)                                                                                                           agaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATa                                                                     >  1:1080043/1‑77 (MQ=255)                                                                                                           agaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCtttt       >  2:1564959/1‑139 (MQ=255)                                                                                                            gaTAATCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTc      >  2:87666/1‑139 (MQ=255)                                                                                                               aaTCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGc   <  1:499694/139‑1 (MQ=255)                                                                                                                aTCGGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCa  <  1:1928271/139‑1 (MQ=255)                                                                                                                   |                                                                                                                                        AGAAACGCCCCAGCGGCGGATTTTTCCCTGGGCGATCAATTTTTCCATCGCTGCGACAGTCTCTTCAAAAGCGAAACTGCCAGACCAGTGTAATAAGTAAAGATCGAGATAATCAGTATTGAGACGGCGTAAACTGGCTTCGCATGCATTTATCGCTTTTTGCCCGCCAGCATTCCACGGATAGACTTTAGAGACGAGAAAGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCA  >  NC_000913/1865211‑1865460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑