Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,874,798	G→A	*149* (TAG→TAA)  T260I (ACT→ATT)	yeaL → yeaM ←	UPF0756 family putative inner membrane protein putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,874,798	0	G	A	100.0%	152.3 / NA	47	*149* (TAG→TAA)  T260I (ACT→ATT)	yeaL yeaM	UPF0756 family putative inner membrane protein putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/28);  total (19/28)

AATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACT  >  NC_000913/1874666‑1874896                                                                                                                                     |                                                                                                   aaTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtc                                                                                              <  1:1405733/139‑1 (MQ=255)  aTGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtct                                                                                             <  1:1069481/139‑1 (MQ=255)       cagccagcCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCaa                                                                                        >  1:203488/1‑139 (MQ=255)          ccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACGGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAatat                                                                                     >  2:1889916/1‑139 (MQ=255)          ccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGc                                                                                          <  2:1978683/134‑1 (MQ=255)          ccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGc                                                                                          >  1:1978683/1‑134 (MQ=255)          ccagccGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAatat                                                                                     >  2:1837565/1‑139 (MQ=255)                  aaCTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCt                                                                             <  2:203488/139‑1 (MQ=255)                       gTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGt                                                                        >  2:1137070/1‑139 (MQ=255)                        tCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                       <  1:1137070/139‑1 (MQ=255)                        tCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                       >  2:916720/1‑139 (MQ=255)                        tCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                       >  2:1513736/1‑139 (MQ=255)                        tCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                       <  1:1971767/139‑1 (MQ=255)                        tCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                       <  1:1953921/139‑1 (MQ=255)                                  gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             <  1:1513736/139‑1 (MQ=255)                                  gctgGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             <  1:807977/139‑1 (MQ=255)                                            aCTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                   <  2:1916232/139‑1 (MQ=255)                                            aCTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                   <  1:802661/139‑1 (MQ=255)                                             cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGtaattaat                                                                                               >  1:758974/1‑94 (MQ=255)                                             cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGtaattaat                                                                                               <  2:758974/94‑1 (MQ=255)                                             cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                         <  2:2012050/100‑1 (MQ=255)                                             cTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCa                                                                                         >  1:2012050/1‑100 (MQ=255)                                                ttttAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             >  1:1762507/1‑125 (MQ=255)                                                ttttAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGc                                                             <  2:1762507/125‑1 (MQ=255)                                                          aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCtt                                                      <  1:371714/122‑1 (MQ=255)                                                          aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCtt                                                      >  2:371714/1‑122 (MQ=255)                                                          aGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAgg                                     >  1:230815/1‑139 (MQ=255)                                                             gcTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCg                                  <  1:2009138/139‑1 (MQ=255)                                                                gTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttg                               <  1:916720/139‑1 (MQ=255)                                                                 tttCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCgttgt                              <  1:1837565/139‑1 (MQ=255)                                                                           tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAAtc                                                                                              >  1:1381204/1‑65 (MQ=255)                                                                           tACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACACTAATTAAtc                                                                                              <  2:1381204/65‑1 (MQ=255)                                                                              cGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCACCAGGCCTTTATTAAACATAGTAATAAAGGCCGTTGTTGAATCATAcccc                 <  1:1889916/139‑1 (MQ=255)                                                                                   gggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                   >  2:200541/1‑100 (MQ=255)                                                                                   gggCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTaaa                                                   <  1:200541/100‑1 (MQ=255)                                                                                      ccGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCtt                                                      <  1:56593/94‑1 (MQ=255)                                                                                      ccGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCtt                                                      >  2:56593/1‑94 (MQ=255)                                                                                          ttATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGTCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGATTCATACCCCAGCGTATGAgaa     <  1:506557/139‑3 (MQ=255)                                                                                             ttGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACt  <  2:230815/139‑1 (MQ=255)                                                                                                                gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCt                                                                             <  1:730889/45‑1 (MQ=255)                                                                                                                gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCt                                                                             >  2:730889/1‑45 (MQ=255)                                                                                                                gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGc                                    <  1:61704/86‑1 (MQ=255)                                                                                                                gCTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGc                                    >  2:61704/1‑86 (MQ=255)                                                                                                                 cTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCttt                                                         >  1:1847995/1‑64 (MQ=255)                                                                                                                 cTGATTGTGGGGAAACAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCttt                                                         <  2:1847995/64‑1 (MQ=255)                                                                                                                              aaCAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                       >  1:1519302/1‑37 (MQ=255)                                                                                                                              aaCAGTAATTAATCTCGCAATATATCGGCCTGGCGtt                                                                       <  2:1519302/37‑1 (MQ=255)                                                                                                                              aaCAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGACCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGaa                          <  2:1996036/82‑1 (MQ=255)                                                                                                                              aaCAGTAATTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGACCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGaa                          >  1:1996036/1‑82 (MQ=255)                                                                                                                                     |                                                                                                   AATGGGCAGCCAGCCGCAACTGGTCGCCGGGCTGCTGGTAGGCACTGTTTTAGGCGTAGCGCTGTTTCGCGGCGTACCGGTTGGGCCGCTTATTGCCGCGGGTCTGGTTTCGCTGATTGTGGGGAAACAGTAGTTAATCTCGCAATATATCGGCCTGGCGTTTGCCCCAGGCCTTTCTTAAACATAGTAATAAAGGCCGTTGTTGAATCATACCCCAGCGTATGAGCAACT  >  NC_000913/1874666‑1874896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑