Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,980,188	C→T	*475* (TAG→TAA)	otsA ←	trehalose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,980,188	0	C	T	100.0%	167.5 / NA	52	*475* (TAG→TAA)	otsA	trehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/28);  total (24/28)

ATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTC  >  NC_000913/1980054‑1980316                                                                                                                                       |                                                                                                                                 atcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACg                                                                                                                              >  1:1364723/1‑139 (MQ=255) atcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACg                                                                                                                              <  1:306850/139‑1 (MQ=255) atcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACg                                                                                                                              >  1:1702943/1‑139 (MQ=255)  tcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc                                                                                                                             <  2:141036/139‑1 (MQ=255)  tcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc                                                                                                                             <  1:31379/139‑1 (MQ=255)  tcatTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc                                                                                                                             <  2:1037985/139‑1 (MQ=255)      ttCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGc                                                                                                                         <  1:2044051/139‑1 (MQ=255)              ggTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGaaa                                                                                                                 >  1:594727/1‑139 (MQ=255)              ggTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGaaa                                                                                                                 <  2:1828100/139‑1 (MQ=255)                              cacaatcacaGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATcg                                                                                                 <  2:1702943/139‑1 (MQ=255)                                       aGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTgg                                                                                        >  1:1389122/1‑139 (MQ=255)                                           tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACg                                                                                                                              <  2:100575/97‑1 (MQ=255)                                           tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACg                                                                                                                              >  1:100575/1‑97 (MQ=255)                                           tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc                                                                                                                             <  1:1617728/98‑1 (MQ=255)                                           tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGc                                                                                                                             >  2:1617728/1‑98 (MQ=255)                                           tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGt                                                                                                              <  2:412702/113‑1 (MQ=255)                                           tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGt                                                                                                              >  1:412702/1‑113 (MQ=255)                                                tCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCcgcg                                                                               <  2:1578783/139‑1 (MQ=255)                                                       cATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcgcg                                                                        <  2:1364723/139‑1 (MQ=255)                                                          ccTGCTCGGCGAAAAGAGTTATTGCCTCATGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGTTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                     <  2:1389122/139‑1 (MQ=255)                                                            tGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACt                                                                   >  1:1715009/1‑139 (MQ=255)                                                                      aaaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAg                                                                                                            <  1:1506512/88‑1 (MQ=255)                                                                      aaaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAg                                                                                                            >  2:1506512/1‑88 (MQ=255)                                                                       aaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAg                                                        <  1:1717730/139‑1 (MQ=255)                                                                       aaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAg                                                        <  2:1944680/139‑1 (MQ=255)                                                                        agagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGaa                                                                                                                  >  1:940034/1‑80 (MQ=255)                                                                        agagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGaa                                                                                                                  <  2:940034/80‑1 (MQ=255)                                                                         gagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGt                                                      <  2:1715009/139‑1 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCt                                                                             <  2:933911/96‑1 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCt                                                                             >  1:933911/1‑96 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                     >  2:223957/1‑104 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                     <  1:223957/104‑1 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGaa                                            >  1:814646/1‑129 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGaa                                            <  2:814646/129‑1 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAg                                           >  2:1282413/1‑130 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAg                                           <  1:1282413/130‑1 (MQ=255)                                                                                             gTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGc                                  >  1:1417833/1‑139 (MQ=255)                                                                                               cGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCa                                <  2:594727/139‑1 (MQ=255)                                                                                                  tgtgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTg                             >  1:1119975/1‑139 (MQ=255)                                                                                                      cTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCgcgc                                                                                              <  1:1961378/70‑1 (MQ=255)                                                                                                      cTGTTAGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCgcgc                                                                                              >  2:1961378/1‑70 (MQ=255)                                                                                                           aGTTCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATc                    >  1:347120/1‑139 (MQ=255)                                                                                                              tCCACTTACGGGAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGtt                 >  1:936584/1‑139 (MQ=255)                                                                                                                       gggAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATg                                              <  2:168727/101‑1 (MQ=255)                                                                                                                       gggAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATg                                              >  1:168727/1‑101 (MQ=255)                                                                                                                       gggAGATTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGAt        >  1:1496561/1‑139 (MQ=255)                                                                                                                            aTTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc                                          <  2:1678688/100‑1 (MQ=255)                                                                                                                            aTTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGc                                          >  1:1678688/1‑100 (MQ=255)                                                                                                                            aTTAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGt   >  1:226695/1‑139 (MQ=255)                                                                                                                             ttAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                     >  2:561797/1‑72 (MQ=255)                                                                                                                             ttAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                     <  1:561797/72‑1 (MQ=255)                                                                                                                             ttAACCGCTCTTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTc  <  2:1417833/139‑1 (MQ=255)                                                                                                                                    ctctTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCt                                                                             >  2:1630796/1‑57 (MQ=255)                                                                                                                                    ctctTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCt                                                                             <  1:1630796/57‑1 (MQ=255)                                                                                                                                    ctctTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCg                                                    <  1:376700/82‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 ATCATTTCGATTTGGTGATTTGCGGTAATCACAATCACAGTTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTC  >  NC_000913/1980054‑1980316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑