Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,964	C→T	*317* (TAG→TAA)	cbl ←	ssuEADCB/tauABCD operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,964	0	C	T	100.0%	198.3 / NA	61	*317* (TAG→TAA)	cbl	ssuEADCB/tauABCD operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/30);  total (31/30)

TTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTC  >  NC_000913/2059828‑2060088                                                                                                                                         |                                                                                                                             ttAAGATGTGCCTCAACAACGTTTCCTCTGTAGTTCAGTCGGTAGAACGGCGGCCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTta                                                                                                                            <  1:595083/139‑1 (MQ=255)     gATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATc                                                                                                                        <  2:471101/139‑1 (MQ=255)           ccTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATaa                                                                                                                  >  2:870786/1‑139 (MQ=255)                               tAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATATCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATaa                                                                                                                  >  2:1867393/1‑119 (MQ=255)                               tAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATATCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATaa                                                                                                                  <  1:1867393/119‑1 (MQ=255)                                  ttCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTc                                                                                           >  2:811005/1‑139 (MQ=255)                                    cAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCa                                                                                         >  1:839815/1‑139 (MQ=255)                                       tCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCa                                                                                      >  2:338192/1‑139 (MQ=255)                                         ggTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAcc                                                                                    >  1:38144/1‑139 (MQ=255)                                          gTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCt                                                                                   <  2:295630/139‑1 (MQ=255)                                                   cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                <  2:947115/117‑1 (MQ=255)                                                   cggACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTg                                                                                                >  1:947115/1‑117 (MQ=255)                                                      aCTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGatat                                                                       <  1:870786/139‑1 (MQ=255)                                                                cGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                             <  2:38144/139‑1 (MQ=255)                                                                  tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCa                                                                                                   >  1:1025199/1‑99 (MQ=255)                                                                  tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCa                                                                                                   <  2:1025199/99‑1 (MQ=255)                                                                  tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                               >  2:719035/1‑103 (MQ=255)                                                                  tATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                               <  1:719035/103‑1 (MQ=255)                                                                   aTGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAg                                                          >  2:1765286/1‑139 (MQ=255)                                                                    tGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGt                                                         >  2:1042432/1‑139 (MQ=255)                                                                     gTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTc                                                        <  1:508607/139‑1 (MQ=255)                                                                        aCTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTg                                                     >  2:135440/1‑139 (MQ=255)                                                                          tGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCa                                                                                                               >  1:811755/1‑79 (MQ=255)                                                                          tGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCa                                                                                                               <  2:811755/79‑1 (MQ=255)                                                                               cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                              <  1:135440/139‑1 (MQ=255)                                                                               cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                              <  1:811005/139‑1 (MQ=255)                                                                               cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                              <  2:1474170/139‑1 (MQ=255)                                                                               cGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATGTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                              >  1:1474170/1‑139 (MQ=255)                                                                                 aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                               <  1:543321/88‑1 (MQ=255)                                                                                 aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGc                                                                                               >  2:543321/1‑88 (MQ=255)                                                                                 aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCa                                                                                         >  1:1487707/1‑94 (MQ=255)                                                                                 aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCa                                                                                         <  2:1487707/94‑1 (MQ=255)                                                                                 aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                           <  1:1752539/108‑1 (MQ=255)                                                                                 aGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTg                                                                           >  2:1752539/1‑108 (MQ=255)                                                                                            aggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                                                                         <  1:1988977/99‑1 (MQ=255)                                                                                            aggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                                                                         >  2:1988977/1‑99 (MQ=255)                                                                                             ggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTtat                                                                                                                           <  1:145070/48‑1 (MQ=255)                                                                                             ggagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTtat                                                                                                                           >  2:145070/1‑48 (MQ=255)                                                                                              gagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                               <  2:839815/139‑1 (MQ=255)                                                                                              gagCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcg                               <  2:1961743/139‑1 (MQ=255)                                                                                               agCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                                                                         >  2:1102678/1‑96 (MQ=255)                                                                                               agCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                                                                         <  1:1102678/96‑1 (MQ=255)                                                                                                gCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGAt                                                                                                                    >  2:905243/1‑52 (MQ=255)                                                                                                gCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGAt                                                                                                                    <  1:905243/52‑1 (MQ=255)                                                                                                gCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGcc                             >  1:1159366/1‑139 (MQ=255)                                                                                                  cAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAcc                                                                                    <  2:65802/82‑1 (MQ=255)                                                                                                  cAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCAcc                                                                                    >  1:65802/1‑82 (MQ=255)                                                                                                        ccTGAAAAGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAg                     >  2:472099/1‑139 (MQ=255)                                                                                                              aaGCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGc               <  1:1042432/139‑1 (MQ=255)                                                                                                                gCCCGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTg             >  2:534127/1‑139 (MQ=255)                                                                                                                 cccGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGa            >  2:868250/1‑139 (MQ=255)                                                                                                                   cGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                                                                         <  1:452577/76‑1 (MQ=255)                                                                                                                   cGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGat                                                                         >  2:452577/1‑76 (MQ=255)                                                                                                                   cGCTTTTATAGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAg          <  1:338192/139‑1 (MQ=255)                                                                                                                           taGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                             >  2:880021/1‑80 (MQ=255)                                                                                                                           taGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGa                                                             <  1:880021/80‑1 (MQ=255)                                                                                                                           taGCGGGATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTc  >  2:536140/1‑139 (MQ=255)                                                                                                                                ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                              <  2:820465/90‑1 (MQ=255)                                                                                                                                ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACa                                              >  1:820465/1‑90 (MQ=255)                                                                                                                                ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                              <  2:8565/106‑1 (MQ=255)                                                                                                                                ggATTTTTGTTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAgcgc                              >  1:8565/1‑106 (MQ=255)                                                                                                                                         |                                                                                                                             TTAAGATGTGCCTCAACAACGATTCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTTAATCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTCCTGAAAAGCCCGCTTTTATAGCGGGATTTTTGCTATATCTGATAATCAATTTCCTCTTCACTGCTTTCCATCACCTGCCGCTTGATATCCTCAACTGACAGTCCTGCATTACAAAGTTCCAGAAAGCGCCAGACATAGTTACGCTGAAGTTGTCCTC  >  NC_000913/2059828‑2060088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑