Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,907	C→T	*407* (TAG→TAA)	wcaL ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,907	0	C	T	100.0%	111.6 / NA	36	*407* (TAG→TAA)	wcaL	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/17);  total (19/17)

TGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCG  >  NC_000913/2115771‑2116041                                                                                                                                         |                                                                                                                                       tgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTta                                                                                                                                      >  2:759408/1‑139 (MQ=255) tgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTta                                                                                                                                      >  2:1733505/1‑139 (MQ=255)   ggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCTGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTtata                                                                                                                                    <  1:1197557/139‑1 (MQ=255)       gTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGc                                                                                                                                <  1:177158/139‑1 (MQ=255)                      aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 <  1:1733505/139‑1 (MQ=255)                       aCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCg                                                                                                                >  2:572091/1‑139 (MQ=255)                           tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGtt                                                                                                            <  1:1055/139‑1 (MQ=255)                            ttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgcag                                                                                                                          <  1:1379157/124‑1 (MQ=255)                            ttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgcag                                                                                                                          >  2:1379157/1‑124 (MQ=255)                             tGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                                                                          >  2:86988/1‑139 (MQ=255)                             tGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                                                                          >  1:722050/1‑139 (MQ=255)                                      ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                                                                 <  1:572091/139‑1 (MQ=255)                                            gcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                                                                          <  1:602745/124‑1 (MQ=255)                                            gcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                                                                          >  2:602745/1‑124 (MQ=255)                                            gcgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgc                                                                                           <  1:86988/139‑1 (MQ=255)                                             cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                         <  1:1722111/124‑1 (MQ=255)                                             cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                                                                         >  2:1722111/1‑124 (MQ=255)                                                 ggCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgcagc                                                                                                                         >  1:2034967/1‑104 (MQ=255)                                                 ggCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTgcagc                                                                                                                         <  2:2034967/104‑1 (MQ=255)                                                  gCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTa                                                                                     <  2:722050/139‑1 (MQ=255)                                                            tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 >  1:207473/1‑101 (MQ=255)                                                            tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGc                                                                                                                 <  2:207473/101‑1 (MQ=255)                                                                   gaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGtt                                                                           >  1:1635249/1‑132 (MQ=255)                                                                   gaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGtt                                                                           <  2:1635249/132‑1 (MQ=255)                                                                    aaGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACtttt                                                                   >  2:1611966/1‑139 (MQ=255)                                                                             gcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcg                                                          >  1:651750/1‑139 (MQ=255)                                                                                            aGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGa                                           >  1:1800014/1‑139 (MQ=255)                                                                                                      gTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATtcgt                                 <  2:1800014/139‑1 (MQ=255)                                                                                                              agTTTTTTAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTcc                         >  2:1579524/1‑139 (MQ=255)                                                                                                                    ttAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcg                                                              <  2:1470227/96‑1 (MQ=255)                                                                                                                    ttAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAAGCGTGTTCAACTTTTTcgcg                                                              >  1:1470227/1‑96 (MQ=255)                                                                                                                     tAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                                                             >  1:79150/1‑80 (MQ=255)                                                                                                                     tAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                                                             <  2:79150/80‑1 (MQ=255)                                                                                                                     tAAATGGCATGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGc                  >  1:1501922/1‑139 (MQ=255)                                                                                                                             aTGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            <  2:1447710/57‑1 (MQ=255)                                                                                                                             aTGCGTTCTCCTTTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                                                            >  1:1447710/1‑57 (MQ=255)                                                                                                                                     tccttTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCg  >  1:1672064/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       TGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCGGAGCCAATTCGTCGGTGTCCAGTTGGCTAAACGCCGCCAGTCG  >  NC_000913/2115771‑2116041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑