Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,118,164	G→A	R81C (CGT→TGT)	wcaK ←	colanic acid biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,118,164	0	G	A	100.0%	183.4 / NA	56	R81C (CGT→TGT)	wcaK	colanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/26);  total (30/26)

TGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATCC  >  NC_000913/2118044‑2118297                                                                                                                         |                                                                                                                                      tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACagag                                                                                                                                    <  1:1149141/124‑1 (MQ=255) tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAAcgcg                                                                                                                     <  2:1099012/139‑1 (MQ=255) tGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCAGCGACagag                                                                                                                                    >  2:1149141/1‑124 (MQ=255)  ggCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACgcgc                                                                                                                    <  2:472274/139‑1 (MQ=255)       cgtAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGAc                                                                                                               <  1:482739/139‑1 (MQ=255)               gACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGTCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccg                                                                                                       <  1:464058/139‑1 (MQ=255)                aCAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccgc                                                                                                      >  1:579741/1‑139 (MQ=255)                  agcagGCGCACGAAATCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTAACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCccgccg                                                                                                    >  1:346034/1‑139 (MQ=255)                    cagGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                  <  1:552714/139‑1 (MQ=255)                                    gTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCa                                                                                  <  2:579741/139‑1 (MQ=255)                                                   atggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCg                                                                   <  2:1470679/139‑1 (MQ=255)                                                    tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTg                                                                              <  1:2040749/127‑1 (MQ=255)                                                    tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTg                                                                              >  2:2040749/1‑127 (MQ=255)                                                    tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCgg                                                                  >  2:866758/1‑139 (MQ=255)                                                    tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCgg                                                                  >  1:267088/1‑139 (MQ=255)                                                    tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCgg                                                                  >  2:1885209/1‑139 (MQ=255)                                                    tggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCgg                                                                  >  2:795999/1‑139 (MQ=255)                                                     ggcgatATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGa                                                                 >  2:1081674/1‑139 (MQ=255)                                                         atatTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccg                                                                                                       >  1:2040028/1‑97 (MQ=255)                                                         atatTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccg                                                                                                       <  2:2040028/97‑1 (MQ=255)                                                          tatTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGcc                                                            >  2:1657555/1‑139 (MQ=255)                                                                       gCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccg                                                                                                       <  2:953102/83‑1 (MQ=255)                                                                       gCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAAcgcccg                                                                                                       >  1:953102/1‑83 (MQ=255)                                                                         cAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACagagga                                                                                                                                  <  2:1472117/54‑1 (MQ=255)                                                                         cAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACagagga                                                                                                                                  >  1:1472117/1‑54 (MQ=255)                                                                              tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg                                                                                          <  2:1704692/89‑1 (MQ=255)                                                                              tCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg                                                                                          >  1:1704692/1‑89 (MQ=255)                                                                               cGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGagcag                                       <  1:1885209/139‑1 (MQ=255)                                                                                ggTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGAc                                                                                                               >  2:1447356/1‑66 (MQ=255)                                                                                ggTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGAc                                                                                                               <  1:1447356/66‑1 (MQ=255)                                                                                 gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGcc                                     >  1:1997425/1‑139 (MQ=255)                                                                                 gTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGcc                                     >  2:583066/1‑139 (MQ=255)                                                                                      gcgTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaa                                >  2:376802/1‑139 (MQ=255)                                                                                        gTGAGAGCAATACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGgaaga                              <  1:583066/139‑1 (MQ=255)                                                                                                  tACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg                                                                                          <  1:1763863/69‑1 (MQ=255)                                                                                                  tACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgtg                                                                                          >  2:1763863/1‑69 (MQ=255)                                                                                                  tACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAg                                                                     >  1:1895709/1‑90 (MQ=255)                                                                                                  tACCTGATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAg                                                                     <  2:1895709/90‑1 (MQ=255)                                                                                                       gATGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCg               >  2:1339383/1‑139 (MQ=255)                                                                                                        aTGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgt                                                                                           <  1:1467502/62‑1 (MQ=255)                                                                                                        aTGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgttgt                                                                                           >  2:1467502/1‑62 (MQ=255)                                                                                                         tGCTGGTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGc             >  2:642368/1‑139 (MQ=255)                                                                                                             ggTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcat         >  2:1751955/1‑139 (MQ=255)                                                                                                             ggTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcat         <  1:1657555/139‑1 (MQ=255)                                                                                                              gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCgcgc                                                                                                 <  1:839022/50‑1 (MQ=255)                                                                                                              gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCgcgc                                                                                                 >  2:839022/1‑50 (MQ=255)                                                                                                              gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAg                 >  2:1840424/1‑130 (MQ=255)                                                                                                              gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAg                 <  1:1840424/130‑1 (MQ=255)                                                                                                              gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcat         >  2:1162423/1‑138 (MQ=255)                                                                                                              gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcat         <  1:1162423/138‑1 (MQ=255)                                                                                                              gTAGCGGCGACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTAGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCtcatc        >  1:1202007/1‑139 (MQ=255)                                                                                                                  cggcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                  >  2:1224980/1‑45 (MQ=255)                                                                                                                  cggcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCcgcg                                                                                                  <  1:1224980/45‑1 (MQ=255)                                                                                                                   ggcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATcac      >  1:559022/1‑136 (MQ=255)                                                                                                                   ggcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATcac      <  2:559022/136‑1 (MQ=255)                                                                                                                    gcgACAGAGGACTTTTTTAACGCGCCCGACAAcgcc                                                                                                         <  2:1846723/36‑1 (MQ=255)                                                                                                                    gcgACAGAGGACTTTTTTAACGCGCCCGACAAcgcc                                                                                                         >  1:1846723/1‑36 (MQ=255)                                                                                                                    gcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgtt                                                                                             >  2:1107054/1‑48 (MQ=255)                                                                                                                    gcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCtgtt                                                                                             <  1:1107054/48‑1 (MQ=255)                                                                                                                    gcgACAGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATcc  <  2:267088/139‑1 (MQ=255)                                                                                                                         |                                                                                                                                      TGGCGTCGTAACCTGACAGCAGGCGCACGAAGTCGGTGAATCCCTGGGCGATGGCGATATTGCGCAGCTTGCCAGTGTCGGTGACGCGTGAGAGCAATACCTGATGCTGGTAGCGGCGACGGAGGACTTTTTTAACGCGCCCGACAACGCCCGCCGCGCTGTTGTGTTGTTTCATTTGCAGGAACAGCGGATCGCCCATTACCGGGCGGTTGAGCAGCCAGGAAGAACTGACCGGATAGCGGCTCATCACATCC  >  NC_000913/2118044‑2118297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑