Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,130,853 C→T S9N (AGC→AAC) 
*406* (TAG→TAA
wcaD ←
wcaC ←
putative colanic acid polymerase
putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,130,8530CT100.0% 209.0 / NA 64S9N (AGC→AAC) 
*406* (TAG→TAA
wcaD
wcaC
putative colanic acid polymerase
putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/33);  total (31/33)

TTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTT  >  NC_000913/2130720‑2130975
                                                                                                                                     |                                                                                                                          
tttAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAg                                                                                                                       <  2:2049916/139‑1 (MQ=255)
  tAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                     <  1:1731959/139‑1 (MQ=255)
        tGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGAt                                                                                                               <  1:29404/139‑1 (MQ=255)
          aTGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAg                                                                                                             >  2:1543165/1‑139 (MQ=255)
             cGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                          <  1:661452/139‑1 (MQ=255)
                   aaaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACa                                                                                                    <  1:1413467/139‑1 (MQ=255)
                    aaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACAt                                                                                                   >  1:563015/1‑139 (MQ=255)
                         agcagTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcc                                                                                              <  2:103216/139‑1 (MQ=255)
                                 cAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCAt                                                                                      <  2:563015/139‑1 (MQ=255)
                                    gACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtg                                                                                   <  2:728523/139‑1 (MQ=255)
                                      cAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgtt                                                                                 <  1:1543165/139‑1 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAtt                                                                                                                    <  1:1135687/101‑1 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAtt                                                                                                                    >  2:1135687/1‑101 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGAt                                                                                                               <  1:247936/106‑1 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGAt                                                                                                               >  2:247936/1‑106 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                            >  1:1603867/1‑109 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                            <  2:1603867/109‑1 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcct                                                                                             <  2:2044060/124‑1 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcct                                                                                             >  1:2044060/1‑124 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                               >  2:867873/1‑138 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                               <  1:867873/138‑1 (MQ=255)
                                         gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTc                                                                              >  1:1205351/1‑139 (MQ=255)
                                         gTAAAAAAGTGACGAGGGGGATGGGGCAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTc                                                                              >  1:529679/1‑139 (MQ=255)
                                                    aCGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcct                                                                                             <  2:1808377/113‑1 (MQ=255)
                                                    aCGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcct                                                                                             >  1:1808377/1‑113 (MQ=255)
                                                           tGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTa                                                                                                                          >  2:1334131/1‑77 (MQ=255)
                                                           tGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTa                                                                                                                          >  1:556142/1‑77 (MQ=255)
                                                           tGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTa                                                                                                                          <  1:1334131/77‑1 (MQ=255)
                                                                    aaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAtt                                                                                                                    <  1:1562541/74‑1 (MQ=255)
                                                                    aaCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAtt                                                                                                                    >  2:1562541/1‑74 (MQ=255)
                                                                     aCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                     >  2:1342060/1‑72 (MQ=255)
                                                                     aCTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGAt                                                                                                                     <  1:1342060/72‑1 (MQ=255)
                                                                         tCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACAt                                                                                                   >  1:2050014/1‑86 (MQ=255)
                                                                         tCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACAt                                                                                                   <  2:2050014/86‑1 (MQ=255)
                                                                                    ggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGa                                                                                                      <  1:471403/72‑1 (MQ=255)
                                                                                    ggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGa                                                                                                      >  2:471403/1‑72 (MQ=255)
                                                                                    ggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAgg                                                                     <  2:366657/105‑1 (MQ=255)
                                                                                    ggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAgg                                                                     >  1:366657/1‑105 (MQ=255)
                                                                                    ggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGt                                   >  2:679540/1‑139 (MQ=255)
                                                                                           ttGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCa                            >  2:1204329/1‑139 (MQ=255)
                                                                                                   aaCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                          <  1:1927016/53‑1 (MQ=255)
                                                                                                   aaCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAg                                                                                                          >  2:1927016/1‑53 (MQ=255)
                                                                                                   aaCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                  >  2:1985449/1‑61 (MQ=255)
                                                                                                   aaCCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                  <  1:1985449/61‑1 (MQ=255)
                                                                                                       agcagATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTgc                <  2:529679/139‑1 (MQ=255)
                                                                                                           gATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtg                                                                                   <  1:2000661/68‑1 (MQ=255)
                                                                                                           gATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtg                                                                                   >  2:2000661/1‑68 (MQ=255)
                                                                                                           gATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGa            >  2:1566356/1‑139 (MQ=255)
                                                                                                              aaaTCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                  <  2:1239663/50‑1 (MQ=255)
                                                                                                              aaaTCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                                  >  1:1239663/1‑50 (MQ=255)
                                                                                                              aaaTCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActc                                                                                               <  2:1475402/53‑1 (MQ=255)
                                                                                                              aaaTCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActc                                                                                               >  1:1475402/1‑53 (MQ=255)
                                                                                                              aaaTCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGc                                                   <  2:1981226/97‑1 (MQ=255)
                                                                                                              aaaTCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGc                                                   >  1:1981226/1‑97 (MQ=255)
                                                                                                                aTCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTcc       >  1:502029/1‑139 (MQ=255)
                                                                                                                 tCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCg      <  2:1205351/139‑1 (MQ=255)
                                                                                                                   aGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTg                                  <  2:108982/109‑1 (MQ=255)
                                                                                                                   aGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTg                                  >  1:108982/1‑109 (MQ=255)
                                                                                                                   aGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGt    <  1:679540/139‑1 (MQ=255)
                                                                                                                     cGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCg                                    <  2:1163357/105‑1 (MQ=255)
                                                                                                                     cGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCg                                    >  1:1163357/1‑105 (MQ=255)
                                                                                                                     cGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGttt  >  1:1084047/1‑139 (MQ=255)
                                                                                                                       gcagcaACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCg                                    >  1:81555/1‑103 (MQ=255)
                                                                                                                       gcagcaACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCg                                    <  2:81555/103‑1 (MQ=255)
                                                                                                                                     |                                                                                                                          
TTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGGTTT  >  NC_000913/2130720‑2130975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: