Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,333,691 A→G pseudogene (712/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,333,6910AG100.0% 288.6 / NA 82pseudogene (712/4605 nt)yfaSpseudogene, bacterial alpha2‑macroglobulin YfaS variant family, putative membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/42);  total (40/42)

CTGTTTTTTACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAGTGGATAACGGA  >  NC_000913/2333569‑2333825
                                                                                                                          |                                                                                                                                      
cTGTTTTTTACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCttgt                                                                                                                        <  2:1174706/139‑1 (MQ=255)
cTGTTTTTTACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCttgt                                                                                                                        <  2:1653229/139‑1 (MQ=255)
    tttttACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGc                                                                                                                    >  2:825964/1‑139 (MQ=255)
        tACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  1:825964/139‑1 (MQ=255)
        tACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  2:1663610/139‑1 (MQ=255)
        tACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  1:1948706/139‑1 (MQ=255)
        tACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  2:52720/139‑1 (MQ=255)
         aCCCGCGGTCCACACCAGAAGCTCTTTGCCTTACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGc                                                                                                                              <  2:1764918/124‑1 (MQ=255)
         aCCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGAGCAGTCCCGGc                                                                                                                              >  1:1764918/1‑124 (MQ=255)
               ggTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAata                                                                                                         >  1:1211105/1‑139 (MQ=255)
                gTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAatat                                                                                                        <  2:1211105/139‑1 (MQ=255)
                           gaGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTc                                                                                                                                   >  1:1286504/2‑101 (MQ=255)
                           aaGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGTCTCGACGAGGTGCAGTc                                                                                                                                   <  2:1286504/101‑1 (MQ=255)
                           aaGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              <  2:1324529/122‑1 (MQ=255)
                           aaGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              >  1:1324529/1‑122 (MQ=255)
                             gCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCgg                                                                                                                               <  1:985213/103‑1 (MQ=255)
                             gCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCgg                                                                                                                               >  2:985213/1‑103 (MQ=255)
                                ctTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTcc                                                                                                                                  <  2:495227/97‑1 (MQ=255)
                                ctTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTcc                                                                                                                                  >  1:495227/1‑97 (MQ=255)
                                ctTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTcc                                                                                                                                  <  2:1521460/97‑1 (MQ=255)
                                ctTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTcc                                                                                                                                  >  1:1521460/1‑97 (MQ=255)
                                ctTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTg                                                                                        >  2:1989663/1‑139 (MQ=255)
                                  ttGCCTTACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcg                                                                                      >  1:860040/1‑139 (MQ=255)
                                  ttGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGcc                                                                                                                   <  2:829849/110‑1 (MQ=255)
                                  ttGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGcc                                                                                                                   >  1:829849/1‑110 (MQ=255)
                                  ttGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCt                                                                                         >  2:799641/1‑136 (MQ=255)
                                  ttGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCt                                                                                         <  1:799641/136‑1 (MQ=255)
                                  ttGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcg                                                                                      >  2:670667/1‑139 (MQ=255)
                                   tGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcgg                                                                                     >  2:1132404/1‑139 (MQ=255)
                                             tttGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGcc                                                                                                                   >  2:2009046/1‑99 (MQ=255)
                                             tttGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGcc                                                                                                                   <  1:2009046/99‑1 (MQ=255)
                                               tGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              >  1:733520/1‑102 (MQ=255)
                                               tGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              <  2:733520/102‑1 (MQ=255)
                                                     gcgcCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGa                                                                   >  2:777431/1‑139 (MQ=255)
                                                       gcCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATg                                                                 <  1:586672/139‑1 (MQ=255)
                                                         cACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgcg                                                               >  1:1364387/1‑139 (MQ=255)
                                                         cACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgcg                                                               <  1:1932061/139‑1 (MQ=255)
                                                         cACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgcg                                                               <  2:860040/139‑1 (MQ=255)
                                                           cGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGcc                                                             >  2:927561/1‑139 (MQ=255)
                                                                tCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcggcg                                                                                   >  1:670615/1‑112 (MQ=255)
                                                                tCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcggcg                                                                                   <  2:670615/112‑1 (MQ=255)
                                                                tCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGACCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGAGCCTTCCa                                                        >  2:1223280/1‑139 (MQ=255)
                                                                  ggAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGt                                                      >  1:671599/1‑139 (MQ=255)
                                                                   gAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCga                                                                                  >  1:994866/1‑110 (MQ=255)
                                                                   gAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCga                                                                                  <  2:994866/110‑1 (MQ=255)
                                                                     aacaaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGtttt                                                   <  1:777431/139‑1 (MQ=255)
                                                                       caaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTc                                                                                                                                   <  1:748104/57‑1 (MQ=255)
                                                                       caaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTc                                                                                                                                   >  2:748104/1‑57 (MQ=255)
                                                                        aaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTc                                                          >  2:653825/1‑129 (MQ=255)
                                                                        aaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTc                                                          <  1:653825/129‑1 (MQ=255)
                                                                              caccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              <  1:1758745/71‑1 (MQ=255)
                                                                              caccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              >  2:1758745/1‑71 (MQ=255)
                                                                              caccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAatat                                                                                                        >  2:1620396/1‑77 (MQ=255)
                                                                              caccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAatat                                                                                                        <  1:1620396/77‑1 (MQ=255)
                                                                                 cgtcgtCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgcg                                                               <  2:558029/115‑1 (MQ=255)
                                                                                 cgtcgtCGCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgcg                                                               >  1:558029/1‑115 (MQ=255)
                                                                                   tcgtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCgg                                     >  2:995665/1‑139 (MQ=255)
                                                                                     gtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  1:720956/62‑1 (MQ=255)
                                                                                     gtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                >  2:720956/1‑62 (MQ=255)
                                                                                        gCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcgg                                                                                     >  1:252273/1‑86 (MQ=255)
                                                                                        gCCCGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcgg                                                                                     <  2:252273/86‑1 (MQ=255)
                                                                                           cGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              >  2:1117023/1‑58 (MQ=255)
                                                                                           cGATACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGagag                                                                                                              <  1:1117023/58‑1 (MQ=255)
                                                                                             aTACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  1:1555452/54‑1 (MQ=255)
                                                                                             aTACCCACCAACCATCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                >  2:1555452/1‑54 (MQ=255)
                                                                                                    ccaaccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCt                    >  2:211397/1‑139 (MQ=255)
                                                                                                     caaccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTg                   <  2:671599/139‑1 (MQ=255)
                                                                                                       accaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCTAGTTTTACCCCTTGCTGCGGCTCGAATGGCTTAGCCTGcc                 >  2:1074738/1‑139 (MQ=255)
                                                                                                        ccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                >  1:665169/1‑43 (MQ=255)
                                                                                                        ccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCgag                                                                                                                <  2:665169/43‑1 (MQ=255)
                                                                                                        ccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcgg                                                                                     <  2:1341854/70‑1 (MQ=255)
                                                                                                        ccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTgcgg                                                                                     >  1:1341854/1‑70 (MQ=255)
                                                                                                        ccaTCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCa                <  1:211397/139‑1 (MQ=255)
                                                                                                           tCGCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAg             <  1:927561/139‑1 (MQ=255)
                                                                                                             gCCTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAGTg           <  1:670667/139‑1 (MQ=255)
                                                                                                               cTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGaa                                                                                                           >  2:1516202/1‑41 (MQ=255)
                                                                                                               cTCGACGAGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGaa                                                                                                           <  1:1516202/41‑1 (MQ=255)
                                                                                                                 cgacgaGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCt                                                            >  2:2025764/1‑86 (MQ=255)
                                                                                                                 cgacgaGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCt                                                            <  1:2025764/86‑1 (MQ=255)
                                                                                                                  gacgaGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCAGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCa                <  2:1216479/129‑1 (MQ=255)
                                                                                                                  gacgaGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCAGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCa                >  1:1216479/1‑129 (MQ=255)
                                                                                                                      aGGTGCAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAGTGGATAACGGa  <  1:995665/139‑1 (MQ=255)
                                                                                                                          |                                                                                                                                      
CTGTTTTTTACCCGCGGTCCACACCAGAAGCTCTTTGCCTGACACTTTGCTAAGCGCCACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCCATAGTGGATAACGGA  >  NC_000913/2333569‑2333825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: