Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,576,407	T→C	E657E (GAA→GAG)	maeB ←	malic enzyme: putative oxidoreductase/phosphotransacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,576,407	0	T	C	100.0%	238.3 / NA	66	E657E (GAA→GAG)	maeB	malic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/27);  total (39/27)

CCATGTTCGGCATCACCAGAATATTGGCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCATCAA  >  NC_000913/2576277‑2576538                                                                                                                                   |                                                                                                                                    ccATGTTCGGCATCACCAGAATATTGGCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGAc                                                                                                                             <  2:1630782/139‑1 (MQ=255)        cGGCATCACCAGAATATTGGCTGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTcc                                                                                                                      >  1:1433660/1‑139 (MQ=255)        cGGCATCACCAGAATATTGGCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTcc                                                                                                                      >  1:393290/1‑139 (MQ=255)        cGGCATCACCAGAATATTGGCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTcc                                                                                                                      >  1:735018/1‑139 (MQ=255)        cGGCATCACCAGAATATTGGCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTcc                                                                                                                      >  2:170441/1‑139 (MQ=255)                ccAGAATATTGGCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTCCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTg                                                                                                              >  2:2012728/1‑139 (MQ=255)                         tGGCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCAtttt                                                                                                     >  1:844414/1‑139 (MQ=255)                           gCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGc                                                                                                   >  1:2038570/1‑139 (MQ=255)                            cGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCt                                                                                                  <  2:1735216/139‑1 (MQ=255)                              gAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGAc                                                                                                                             >  2:1322192/1‑110 (MQ=255)                              gAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGAc                                                                                                                             <  1:1322192/110‑1 (MQ=255)                              gAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcg                                                                                                >  2:10539/1‑139 (MQ=255)                              gAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTcg                                                                                                >  2:346902/1‑139 (MQ=255)                                    ttCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGacgacg                                                                                          <  2:1433660/139‑1 (MQ=255)                                     tCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACgg                                                                                         >  2:317727/1‑139 (MQ=255)                                      cAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACggg                                                                                        <  1:10539/139‑1 (MQ=255)                                      cAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACggg                                                                                        <  2:1123573/139‑1 (MQ=255)                                             cTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCag                                                                                 <  2:735018/139‑1 (MQ=255)                                                tCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGAc                                                                                                            >  2:1425692/1‑109 (MQ=255)                                                tCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGAc                                                                                                            <  1:1425692/109‑1 (MQ=255)                                                tCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCagaag                                                                              >  2:114201/1‑139 (MQ=255)                                                tCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCagaag                                                                              >  1:184379/1‑139 (MQ=255)                                                   ggCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAAc                                                                           <  2:2038570/139‑1 (MQ=255)                                                    gCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAAcc                                                                          <  1:170441/139‑1 (MQ=255)                                                               ttGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGAc                                                                                                                             <  2:1730059/77‑1 (MQ=255)                                                               ttGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGAc                                                                                                                             >  1:1730059/1‑77 (MQ=255)                                                               ttGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGt                                                               >  2:653143/1‑139 (MQ=255)                                                                   gAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGa                                                           <  1:346902/139‑1 (MQ=255)                                                                    aaTCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGac                                                          >  2:1782145/1‑139 (MQ=255)                                                                      tCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGacaa                                                        >  1:1737045/1‑139 (MQ=255)                                                                         cTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGAcaacaa                                                     >  2:845960/1‑139 (MQ=255)                                                                          ttCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGa                                                           <  2:1023744/132‑1 (MQ=255)                                                                          ttCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGa                                                           >  1:1023744/1‑132 (MQ=255)                                                                             caccaGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCa                                                 <  1:114201/139‑1 (MQ=255)                                                                                        cATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGAc                                                                                                                             <  1:50714/52‑1 (MQ=255)                                                                                        cATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGAc                                                                                                                             >  2:50714/1‑52 (MQ=255)                                                                                        cATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTc                                      <  1:653143/139‑1 (MQ=255)                                                                                        cATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTc                                      >  1:1649064/1‑139 (MQ=255)                                                                                                  cATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCag                                                                                 <  1:1021419/86‑1 (MQ=255)                                                                                                  cATTTCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCag                                                                                 >  2:1021419/1‑86 (MQ=255)                                                                                                     ttCACCATCAATCATCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAacga                         >  1:813309/1‑139 (MQ=255)                                                                                                            tcaatcaTCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCt                                                                                                  <  2:895467/59‑1 (MQ=255)                                                                                                            tcaatcaTCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCt                                                                                                  >  1:895467/1‑59 (MQ=255)                                                                                                            tcaatcaTCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAg                  >  2:555571/1‑139 (MQ=255)                                                                                                            tcaatcaTCAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAg                  >  2:335887/1‑139 (MQ=255)                                                                                                               atcatcAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGttt               >  2:851661/1‑139 (MQ=255)                                                                                                               atcatcAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGttt               >  1:1026365/1‑139 (MQ=255)                                                                                                               atcatcAGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGttt               >  2:2020088/1‑139 (MQ=255)                                                                                                                 catcaGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACggg                                                                                        >  1:280527/1‑64 (MQ=255)                                                                                                                 catcaGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACggg                                                                                        <  2:280527/64‑1 (MQ=255)                                                                                                                   tcaGTTCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgc           <  2:393290/139‑1 (MQ=255)                                                                                                                       ttCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGc                                                                                       >  1:491716/1‑59 (MQ=255)                                                                                                                       ttCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGc                                                                                       <  2:491716/59‑1 (MQ=255)                                                                                                                       ttCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTgccgcc       >  1:208584/1‑139 (MQ=255)                                                                                                                        tCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCa      <  1:2020088/139‑1 (MQ=255)                                                                                                                        tCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCa      >  2:1057256/1‑139 (MQ=255)                                                                                                                        tCTGGTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCa      <  1:1782145/139‑1 (MQ=255)                                                                                                                            gTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGt                                                                     >  2:2038112/1‑72 (MQ=255)                                                                                                                            gTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAg                                                                >  1:1012542/1‑77 (MQ=255)                                                                                                                            gTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAg                                                                <  2:1012542/77‑1 (MQ=255)                                                                                                                            gTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGc                                                             >  1:146136/1‑80 (MQ=255)                                                                                                                            gTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAAcgcg                                            >  2:1541333/1‑97 (MQ=255)                                                                                                                            gTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAAcgcg                                            <  1:1541333/97‑1 (MQ=255)                                                                                                                            gTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCATCaa  >  1:59738/1‑139 (MQ=255)                                                                                                                            gTGCACGCTCCCTGACCAGTTCCAGCGCCTGACGCATGTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGt                                                                     <  1:2038112/72‑1 (MQ=255)                                                                                                                            gTGCACGCGCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGc                                                             <  2:146136/80‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    CCATGTTCGGCATCACCAGAATATTGGCGGAACCTTTCAAAGAGCTGTCCGGCATACGGTCGTTGCGAATCGCTTCCACCAGCGCTGCATCGCCGTGCATTTCACCATCAATCATCAGTTCTGGTGCACGTTCCCTGACCAGTTCCAGCGCCTGACGCATTTTGCTCGACGACGGGCAGTCAGAAGAACCAAAGTTGGAGTGCGACAACAAAGCAACGCGCGGCTCAATACCAAAACGACGGACAGTTTCTGCCGCCATCAA  >  NC_000913/2576277‑2576538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑