Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 253,084 A→G T126A (ACA→GCA)  yafP → GNAT family putative N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913253,0840AG100.0% 192.3 / NA 55T126A (ACA→GCA) yafPGNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/30);  total (25/30)

TTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATG  >  NC_000913/252949‑253198
                                                                                                                                       |                                                                                                                  
ttATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAg                                                                                                                 <  2:1918369/139‑1 (MQ=255)
  aTTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGtt                                                                                                               <  2:957636/139‑1 (MQ=255)
         gACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagc                                                                                                        >  2:685101/1‑139 (MQ=255)
         gACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagc                                                                                                        <  2:1625025/139‑1 (MQ=255)
          aCCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagca                                                                                                       <  2:1912171/139‑1 (MQ=255)
          aCCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagca                                                                                                       >  1:911824/1‑139 (MQ=255)
                aaTACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGt                                                                                                 >  2:303382/1‑139 (MQ=255)
                 aTACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGtt                                                                                                <  2:911824/139‑1 (MQ=255)
                     acCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAAt                                                                                            >  1:2015089/1‑139 (MQ=255)
                          ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagca                                                                                                       >  1:1325730/1‑123 (MQ=255)
                          ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAagcagca                                                                                                       <  2:1325730/123‑1 (MQ=255)
                          ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAag                                                                                                            <  2:1783427/118‑1 (MQ=255)
                          ccgTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAag                                                                                                            >  1:1783427/1‑118 (MQ=255)
                              ggggTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAg                                                                                   <  2:1651191/139‑1 (MQ=255)
                               gggTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTAACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGc                                                                                  <  1:1239242/139‑1 (MQ=255)
                                ggTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                 <  1:1017826/139‑1 (MQ=255)
                                         cgcTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGa                                                                                              <  2:953214/117‑1 (MQ=255)
                                         cgcTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGa                                                                                              >  1:953214/1‑117 (MQ=255)
                                          gcTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTa                                                                       >  2:2029181/1‑139 (MQ=255)
                                              tGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGa                                                                                              >  1:1287420/1‑112 (MQ=255)
                                              tGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGa                                                                                              <  2:1287420/112‑1 (MQ=255)
                                                    gtataagagacaggTCTGAATCCGAAGTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTatat                                                             <  1:1307175/126‑1 (MQ=255)
                                                          tGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                 >  2:1882601/1‑113 (MQ=255)
                                                          tGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                 <  1:1882601/113‑1 (MQ=255)
                                                                            aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCggg                                                                                      >  1:45958/1‑90 (MQ=255)
                                                                            aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCggg                                                                                      <  2:45958/90‑1 (MQ=255)
                                                                            aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCACAACATTAAAt                                     <  1:2029181/139‑1 (MQ=255)
                                                                            aaCTTACGGTGGACGCAAGCATAACCGCAAAACCCTCTTTTGAACGTGATGGTTTTCAGGCAGGTAAGCAGCAGCGCGTTGAATGCCTGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAAcctgtctcttatac                                     >  2:1307175/1‑127 (MQ=255)
                                                                                 aCGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATcc                                   >  2:1626290/1‑136 (MQ=255)
                                                                                 aCGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATcc                                   <  1:1626290/136‑1 (MQ=255)
                                                                                 aCGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGc                                <  1:1762545/139‑1 (MQ=255)
                                                                                  cGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCt                               <  1:303382/139‑1 (MQ=255)
                                                                                       gACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCa                                              >  1:1681685/1‑119 (MQ=255)
                                                                                       gACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCa                                              <  2:1681685/119‑1 (MQ=255)
                                                                                         cGCAAGCATAACCGCAAAGCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTGGCAATg                        <  1:685101/139‑1 (MQ=255)
                                                                                                 tAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCa                           <  1:856032/128‑1 (MQ=255)
                                                                                                 tAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCa                           >  2:856032/1‑128 (MQ=255)
                                                                                                  aaCCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcg               >  2:675840/1‑139 (MQ=255)
                                                                                                    ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTg                                                                               <  1:1659966/73‑1 (MQ=255)
                                                                                                    ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTg                                                                               >  2:1659966/1‑73 (MQ=255)
                                                                                                    ccGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAAcgcc             <  1:675840/139‑1 (MQ=255)
                                                                                                         aaaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCt        >  1:444770/1‑139 (MQ=255)
                                                                                                          aaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGc                                                                                  >  2:126167/1‑64 (MQ=255)
                                                                                                          aaCCCTTTTTTGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGc                                                                                  <  1:126167/64‑1 (MQ=255)
                                                                                                               ttttttGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCg                                                                                        <  2:550516/53‑1 (MQ=255)
                                                                                                               ttttttGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCg                                                                                        >  1:550516/1‑53 (MQ=255)
                                                                                                               ttttttGAACGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTAtg  >  1:519645/1‑139 (MQ=255)
                                                                                                                      aaCGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATg                                                                                           <  1:1399004/43‑1 (MQ=255)
                                                                                                                      aaCGTTATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATg                                                                                           >  2:1399004/1‑43 (MQ=255)
                                                                                                                           tATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                 >  1:1299589/1‑48 (MQ=255)
                                                                                                                           tATGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCg                                                                                 <  2:1299589/48‑1 (MQ=255)
                                                                                                                            aTGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAAttttt                                                                 <  2:529593/63‑1 (MQ=255)
                                                                                                                            aTGGTTTTCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAAttttt                                                                 >  1:529593/1‑63 (MQ=255)
                                                                                                                                   tCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACAtt                                         <  2:397328/80‑1 (MQ=255)
                                                                                                                                   tCAGGCAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACAtt                                         >  1:397328/1‑80 (MQ=255)
                                                                                                                                       |                                                                                                                  
TTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTTTGTTAAAACCTTTGATTAAGTCTGAATCCGAACTTACGGTGGACGCAAGCATAACCGCAAAACCCTTTTTTGAACGTTATGGTTTTCAGACAGTTAAGCAGCAGCGCGTTGAATGCCGGGGAGCGTGGTTTACTAATTTTTATATGCGATATAAACCGCAACATTAAATCCAGCTTGCAATGAAAATAACGCCCGCCTGGTATG  >  NC_000913/252949‑253198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑