Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,800,475	G→A	*110* (TAG→TAA)	ygaM →	putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,800,475	0	G	A	100.0%	208.9 / NA	64	*110* (TAG→TAA)	ygaM	putative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/33);  total (31/33)

CGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCT  >  NC_000913/2800343‑2800607                                                                                                                                     |                                                                                                                                     cgTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaa                                                                                                                                >  2:1377299/1‑139 (MQ=255)  gTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                               <  2:1904957/139‑1 (MQ=255)    ccAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAAt                                                                                                                             <  1:1301515/139‑1 (MQ=255)                gcgcgATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATAc                                                                                                                 <  2:243309/139‑1 (MQ=255)                                tGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGa                                                                                                 >  2:1830826/1‑139 (MQ=255)                                               gTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCgggg                                                                                  <  1:1830826/139‑1 (MQ=255)                                                   gTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTc                                                                              >  2:1811840/1‑139 (MQ=255)                                                          aCCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATAGTAATGCAAAAATGata                                                                                                                         <  1:925139/89‑1 (MQ=255)                                                          aCCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATAGTAATGCAAAAATGata                                                                                                                         >  2:925139/1‑89 (MQ=255)                                                            ccTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCg                                                                                     <  1:119640/123‑1 (MQ=255)                                                            ccTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCg                                                                                     >  2:119640/1‑123 (MQ=255)                                                              tGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATAc                                                                                                                 >  2:1411143/1‑93 (MQ=255)                                                              tGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATAc                                                                                                                 <  1:1411143/93‑1 (MQ=255)                                                               ggTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                               >  1:1778873/1‑78 (MQ=255)                                                               ggTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                               <  2:1778873/78‑1 (MQ=255)                                                                     gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATca                                                            <  2:1039524/139‑1 (MQ=255)                                                                     gCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATca                                                            >  2:397702/1‑139 (MQ=255)                                                                         gggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaa                                                        <  1:1377299/139‑1 (MQ=255)                                                                          ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                       >  2:306312/1‑139 (MQ=255)                                                                           gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCGCTGTTAAGCATGCGCAAATCGTAATGCaaa                                                                                                                                <  1:1416530/65‑1 (MQ=255)                                                                           gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                               >  2:487331/1‑66 (MQ=255)                                                                           gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaaa                                                                                                                               <  1:487331/66‑1 (MQ=255)                                                                           gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCaaa                                                                                                                                >  2:1416530/1‑65 (MQ=255)                                                                           gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGAccc                                                                                                    >  2:329910/1‑93 (MQ=255)                                                                           gTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGAccc                                                                                                    <  1:329910/93‑1 (MQ=255)                                                                              cagcagCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGt                                                                                 <  1:888451/109‑1 (MQ=255)                                                                              cagcagCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGt                                                                                 >  2:888451/1‑109 (MQ=255)                                                                                    cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAAt                                                                                                                             <  2:130666/59‑1 (MQ=255)                                                                                    cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAAt                                                                                                                             >  1:130666/1‑59 (MQ=255)                                                                                      gCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTAccc                                           >  2:536123/1‑139 (MQ=255)                                                                                               aTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCt                                  <  1:1811840/139‑1 (MQ=255)                                                                                               aTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCt                                  <  2:1862899/139‑1 (MQ=255)                                                                                                        ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                      <  1:101422/126‑1 (MQ=255)                                                                                                        ggCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                      >  2:101422/1‑126 (MQ=255)                                                                                                         gcgcACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGa                                                                                                 <  2:1210336/66‑1 (MQ=255)                                                                                                         gcgcACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGa                                                                                                 >  1:1210336/1‑66 (MQ=255)                                                                                                                 ttAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGt                                                                                 <  1:1380754/74‑1 (MQ=255)                                                                                                                 ttAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGt                                                                                 >  2:1380754/1‑74 (MQ=255)                                                                                                                    aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc                                                                      <  2:2045066/82‑1 (MQ=255)                                                                                                                    aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGcc                                                                      >  1:2045066/1‑82 (MQ=255)                                                                                                                    aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc             <  1:536123/139‑1 (MQ=255)                                                                                                                    aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc             <  1:397702/139‑1 (MQ=255)                                                                                                                    aGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGAGTTTCTTTTTTCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc             >  2:979656/1‑139 (MQ=255)                                                                                                                       aTGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCAc          >  2:924974/1‑139 (MQ=255)                                                                                                                        tGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt         <  1:1825092/139‑1 (MQ=255)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATAcgc                                                                                                               >  2:1205608/1‑36 (MQ=38)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATAcgc                                                                                                               <  1:1205608/36‑1 (MQ=255)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                           >  1:402953/1‑88 (MQ=255)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATcac                                                           <  2:402953/88‑1 (MQ=255)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc             <  2:928161/134‑1 (MQ=255)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGAc             >  1:928161/1‑134 (MQ=255)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa        >  1:433131/1‑139 (MQ=255)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa        >  1:42216/1‑139 (MQ=255)                                                                                                                         gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTa        >  1:2001651/1‑139 (MQ=255)                                                                                                                          cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt       >  1:1507491/1‑139 (MQ=255)                                                                                                                           gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc                                 >  1:1078551/1‑112 (MQ=255)                                                                                                                           gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTc                                 <  2:1078551/112‑1 (MQ=255)                                                                                                                           gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                            <  1:1789473/117‑1 (MQ=255)                                                                                                                           gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATc                            >  2:1789473/1‑117 (MQ=255)                                                                                                                              aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc   <  2:2001651/139‑1 (MQ=255)                                                                                                                               aTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt       <  1:1217590/134‑1 (MQ=255)                                                                                                                               aTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt  <  2:433131/139‑1 (MQ=255)                                                                                                                               aTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCt  <  1:924974/139‑1 (MQ=255)                                                                                                                               aTCGTAATGCAAAAATGAAAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt       >  2:1217590/1‑134 (MQ=255)                                                                                                                                     |                                                                                                                                     CGTCCAGCAAGCCGCGCGCGATGCCGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGCT  >  NC_000913/2800343‑2800607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑