Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,491 C→T *362* (TAG→TAA mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,4910CT100.0% 205.5 / NA 64*362* (TAG→TAAmltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/33);  total (31/33)

TGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGC  >  NC_000913/2824356‑2824612
                                                                                                                                       |                                                                                                                         
tGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAc                                                                                                                        >  2:1035317/1‑139 (MQ=255)
    agtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACttac                                                                                                                    >  1:824267/1‑136 (MQ=255)
                 tGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCgcgccagcgcc                                                                                                      <  1:1676334/139‑1 (MQ=255)
                      gACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                                 <  2:984017/139‑1 (MQ=255)
                      gACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                                 <  2:166667/139‑1 (MQ=255)
                           gATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCgcgcc                                                                                                            >  1:1245744/1‑123 (MQ=255)
                           gATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCgcgcc                                                                                                            <  2:1245744/123‑1 (MQ=255)
                                cACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                                 <  2:405870/129‑1 (MQ=255)
                                cACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                                 >  1:405870/1‑129 (MQ=255)
                                   tttCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGc                                                                                    <  1:1035317/139‑1 (MQ=255)
                                     tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAc                                                                                                                        <  2:984097/102‑1 (MQ=255)
                                     tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAc                                                                                                                        >  1:984097/1‑102 (MQ=255)
                                     tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCa                                                                                  >  2:752705/1‑139 (MQ=255)
                                     tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCa                                                                                  >  2:1200302/1‑139 (MQ=255)
                                       gTCAGGAAGATGGCGTTGGCGTTACAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCACGCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGa                                                                                <  1:1357626/139‑1 (MQ=255)
                                        tCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                               <  1:1377002/139‑1 (MQ=255)
                                        tCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                               <  2:4136/139‑1 (MQ=255)
                                                    tgtTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTa                                                                   >  2:75068/1‑139 (MQ=255)
                                                      ttGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAAt                                                                 >  1:67227/1‑139 (MQ=255)
                                                         gCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                              <  2:67227/139‑1 (MQ=255)
                                                            ttGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCg                                                                             <  2:1567120/121‑1 (MQ=255)
                                                            ttGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCg                                                                             >  1:1567120/1‑121 (MQ=255)
                                                             tGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                          <  1:1645603/139‑1 (MQ=255)
                                                              gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGc                                                                      >  1:615190/1‑126 (MQ=255)
                                                              gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGc                                                                      <  2:615190/126‑1 (MQ=255)
                                                              gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtg                                                         >  2:1946466/1‑139 (MQ=255)
                                                                    ttACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTg                                                   <  1:752705/139‑1 (MQ=255)
                                                                            aggaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGt                                           >  2:1637195/1‑139 (MQ=255)
                                                                              gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                                         <  1:1707466/139‑1 (MQ=255)
                                                                                ttCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                                          >  2:547785/1‑88 (MQ=255)
                                                                                ttCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCt                                                                                          <  1:547785/88‑1 (MQ=255)
                                                                                     aaaaaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTa                                  >  2:879769/1‑139 (MQ=255)
                                                                                        aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGaa                               >  1:667959/1‑139 (MQ=255)
                                                                                               aTTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                              <  1:740756/101‑1 (MQ=255)
                                                                                               aTTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                              >  2:740756/1‑101 (MQ=255)
                                                                                                      agagGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGt                 >  2:1931964/1‑139 (MQ=255)
                                                                                                       gagGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa                <  2:667959/139‑1 (MQ=255)
                                                                                                       gagGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa                <  1:1946466/139‑1 (MQ=255)
                                                                                                        agGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAt                                                                         >  2:1801555/1‑81 (MQ=255)
                                                                                                        agGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAt                                                                         <  1:1801555/81‑1 (MQ=255)
                                                                                                             aGCTCACGCCCCCCTCTTGTAAATAGTTGCTTACTCGCGCCAGCGCCACGGc                                                                                                 >  2:1276398/1‑52 (MQ=37)
                                                                                                             aGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                                 <  1:1276398/52‑1 (MQ=38)
                                                                                                             aGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTg                                                                                              >  1:1979453/1‑55 (MQ=255)
                                                                                                             aGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTg                                                                                              <  2:1979453/55‑1 (MQ=255)
                                                                                                              gCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAt                                                                         <  2:1611595/75‑1 (MQ=255)
                                                                                                              gCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCAt                                                                         >  1:1611595/1‑75 (MQ=255)
                                                                                                                   cGCCCCCCTCTTGTAAATAGTTACTTACTCgcgcc                                                                                                            >  1:59724/1‑35 (MQ=255)
                                                                                                                   cGCCCCCCTCTTGTAAATAGTTACTTACTCgcgcc                                                                                                            <  2:59724/35‑1 (MQ=255)
                                                                                                                    gCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                          <  2:837987/84‑1 (MQ=255)
                                                                                                                    gCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                                          >  1:837987/1‑84 (MQ=255)
                                                                                                                    gCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAg   >  2:184723/1‑139 (MQ=255)
                                                                                                                     ccccccTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGc  <  1:879769/139‑1 (MQ=255)
                                                                                                                         ccTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAAc                                               <  2:1805576/90‑1 (MQ=255)
                                                                                                                         ccTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAAc                                               >  1:1805576/1‑90 (MQ=255)
                                                                                                                              tGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGa                     >  2:594776/1‑111 (MQ=255)
                                                                                                                              tGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGa                     <  1:594776/111‑1 (MQ=255)
                                                                                                                                tAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                               <  1:674434/51‑1 (MQ=38)
                                                                                                                                tAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                               >  2:674434/1‑51 (MQ=38)
                                                                                                                                tAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                               >  1:1731230/1‑51 (MQ=38)
                                                                                                                                tAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                               <  2:1731230/51‑1 (MQ=38)
                                                                                                                                tAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGt                                                             >  2:1287089/1‑69 (MQ=255)
                                                                                                                                tAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGt                                                             <  1:1287089/69‑1 (MQ=255)
                                                                                                                                tAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGa                     >  1:975348/1‑109 (MQ=255)
                                                                                                                                tAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGa                     <  2:975348/109‑1 (MQ=255)
                                                                                                                                       |                                                                                                                         
TGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTACCAGTACTGGTAGC  >  NC_000913/2824356‑2824612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: