Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 273,607 | A→G | pseudogene (761/1108 nt) | ykfC → | CP4‑6 prophage, conserved protein,Phage or Prophage Related |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 273,607 | 0 | A | G | 98.7% | 257.5 / NA | 74 | pseudogene (761/1108 nt) | ykfC | CP4‑6 prophage, conserved protein,Phage or Prophage Related |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (42/31); minor base T (1/0); total (43/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
TTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTG > NC_000913/273474‑273737 | tttCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGa < 1:1875178/139‑1 (MQ=255) ccACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGaa < 1:1759096/116‑1 (MQ=255) ccACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGaa > 2:1759096/1‑116 (MQ=255) ccACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGaa > 1:1780386/1‑139 (MQ=255) ccACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGaa > 2:1130712/1‑139 (MQ=255) cGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTAcggcg > 1:1478099/1‑139 (MQ=255) cGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCAAACGGGGCCGAAGTAcggcg > 2:1848661/1‑139 (MQ=255) ggTGTTCTATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTAcggcgg < 1:530542/139‑1 (MQ=255) tgtTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTc < 1:1130712/139‑1 (MQ=255) ttATATCGCCGCTATTATCGAACATCATGCTGAAGGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCag < 2:1310915/139‑1 (MQ=255) tCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGaaa > 2:1956187/1‑139 (MQ=255) ccgcTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGt > 2:1324313/1‑120 (MQ=255) ccgcTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACt > 1:942745/1‑139 (MQ=255) ccgcTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTCCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGTAGt < 1:1324313/120‑1 (MQ=255) cTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGc > 1:342018/1‑139 (MQ=255) cagtcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAcc < 2:1505239/136‑1 (MQ=255) catcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTgg < 1:462330/127‑1 (MQ=255) catcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTgg > 2:462330/1‑127 (MQ=255) catcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAccc > 2:949110/1‑139 (MQ=255) atcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg < 2:942745/139‑1 (MQ=255) tGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGt > 1:117426/1‑139 (MQ=255) tGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGc < 1:1848661/139‑1 (MQ=255) aTGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTa > 2:277886/1‑139 (MQ=255) gAGTTCGATCAATACCTGCATGAGCGCTACCTGCGCGGCAGAGCCAGAAAAGATCGGTGGTGCTCGAATAACAGTATCCAACGGCGCCGAAGTACACCGGTCAGACGAAACTGGCAGTGGAAACcggcgggagcgcctg > 1:1505239/1‑130 (MQ=255) ttCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTc > 2:1262612/1‑99 (MQ=255) ttCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTc < 1:1262612/99‑1 (MQ=255) aTCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACg > 1:1756061/1‑76 (MQ=255) aTCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACg < 2:1756061/76‑1 (MQ=255) aTCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGt > 1:988212/1‑94 (MQ=255) aTCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGt < 2:988212/94‑1 (MQ=255) tGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCga > 1:1055802/1‑136 (MQ=255) tGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCga < 2:1055802/136‑1 (MQ=255) cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGc < 1:334454/97‑1 (MQ=255) cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGc > 2:334454/1‑97 (MQ=255) cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg > 1:1673419/1‑109 (MQ=255) cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg < 2:1673419/109‑1 (MQ=255) cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt > 1:396694/1‑139 (MQ=255) cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt > 2:575212/1‑139 (MQ=255) cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt < 1:1956187/139‑1 (MQ=255) aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACgggg < 2:1932450/64‑1 (MQ=255) aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACgggg > 1:1932450/1‑64 (MQ=255) aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAcc > 2:1271566/1‑103 (MQ=255) aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAcc < 1:1271566/103‑1 (MQ=255) aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGt > 1:1302041/1‑139 (MQ=255) aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGt > 1:1850879/1‑139 (MQ=255) gcgcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTc < 2:1780386/139‑1 (MQ=255) cgcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGaa < 2:1319401/44‑1 (MQ=255) cgcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGaa > 1:1319401/1‑44 (MQ=255) gcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCt > 1:488963/1‑139 (MQ=255) cTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTTCTGGAATAACAGTATCCAAc > 1:1590317/1‑56 (MQ=255) cTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAAc < 2:1590317/56‑1 (MQ=255) cTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTc > 1:83528/1‑139 (MQ=255) cTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTc < 2:1478099/139‑1 (MQ=255) tACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCa < 1:1528070/139‑1 (MQ=255) aCCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCagag < 1:205992/78‑1 (MQ=255) aCCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCagag > 2:205992/1‑78 (MQ=255) gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCag > 1:1019397/1‑72 (MQ=255) gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCag < 2:1019397/72‑1 (MQ=255) gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCa < 2:624068/134‑1 (MQ=255) gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCa > 1:624068/1‑134 (MQ=255) gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTc > 2:1625489/1‑139 (MQ=255) aGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTcc < 1:583301/130‑1 (MQ=255) aGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTcc > 2:583301/1‑130 (MQ=255) gCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt > 2:233044/1‑123 (MQ=255) gCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt < 1:233044/123‑1 (MQ=255) gCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCaa > 1:1568496/1‑139 (MQ=255) ggAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg > 2:426555/1‑90 (MQ=255) ggAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg < 1:426555/90‑1 (MQ=255) ggAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAgg > 2:819419/1‑139 (MQ=255) aaaGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCaa > 2:454511/1‑42 (MQ=255) aaaGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCaa < 1:454511/42‑1 (MQ=255) aaaGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCa > 2:712117/1‑139 (MQ=255) aTCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGt > 2:1863483/1‑139 (MQ=255) tCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTg > 1:1734435/1‑139 (MQ=255) | TTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTG > NC_000913/273474‑273737 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |