Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 273,607 A→G pseudogene (761/1108 nt) ykfC → CP4‑6 prophage, conserved protein,Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913273,6070AG98.7% 257.5 / NA 74pseudogene (761/1108 nt)ykfCCP4‑6 prophage, conserved protein,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (42/31);  minor base T (1/0);  total (43/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTG  >  NC_000913/273474‑273737
                                                                                                                                     |                                                                                                                                  
tttCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGa                                                                                                                               <  1:1875178/139‑1 (MQ=255)
                        ccACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGaa                                                                                                                              <  1:1759096/116‑1 (MQ=255)
                        ccACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGaa                                                                                                                              >  2:1759096/1‑116 (MQ=255)
                        ccACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGaa                                                                                                       >  1:1780386/1‑139 (MQ=255)
                        ccACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGaa                                                                                                       >  2:1130712/1‑139 (MQ=255)
                                cGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTAcggcg                                                                                               >  1:1478099/1‑139 (MQ=255)
                                cGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCAAACGGGGCCGAAGTAcggcg                                                                                               >  2:1848661/1‑139 (MQ=255)
                                 ggTGTTCTATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTAcggcgg                                                                                              <  1:530542/139‑1 (MQ=255)
                                   tgtTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTc                                                                                            <  1:1130712/139‑1 (MQ=255)
                                     ttATATCGCCGCTATTATCGAACATCATGCTGAAGGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCag                                                                                          <  2:1310915/139‑1 (MQ=255)
                                          tCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGaaa                                                                                     >  2:1956187/1‑139 (MQ=255)
                                             ccgcTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGt                                                                                                     >  2:1324313/1‑120 (MQ=255)
                                             ccgcTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACt                                                                                  >  1:942745/1‑139 (MQ=255)
                                             ccgcTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTCCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGTAGt                                                                                                     <  1:1324313/120‑1 (MQ=255)
                                                cTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGc                                                                               >  1:342018/1‑139 (MQ=255)
                                                          cagtcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAcc                                                                     <  2:1505239/136‑1 (MQ=255)
                                                           catcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTgg                                                                                <  1:462330/127‑1 (MQ=255)
                                                           catcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTgg                                                                                >  2:462330/1‑127 (MQ=255)
                                                           catcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAccc                                                                    >  2:949110/1‑139 (MQ=255)
                                                            atcatGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg                                                                   <  2:942745/139‑1 (MQ=255)
                                                                tGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGt                                                               >  1:117426/1‑139 (MQ=255)
                                                                   tGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGc                                                            <  1:1848661/139‑1 (MQ=255)
                                                                      aTGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTa                                                         >  2:277886/1‑139 (MQ=255)
                                                                        gAGTTCGATCAATACCTGCATGAGCGCTACCTGCGCGGCAGAGCCAGAAAAGATCGGTGGTGCTCGAATAACAGTATCCAACGGCGCCGAAGTACACCGGTCAGACGAAACTGGCAGTGGAAACcggcgggagcgcctg                                                       >  1:1505239/1‑130 (MQ=255)
                                                                           ttCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTc                                                                                            >  2:1262612/1‑99 (MQ=255)
                                                                           ttCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTc                                                                                            <  1:1262612/99‑1 (MQ=255)
                                                                               aTCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACg                                                                                                               >  1:1756061/1‑76 (MQ=255)
                                                                               aTCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACg                                                                                                               <  2:1756061/76‑1 (MQ=255)
                                                                               aTCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGt                                                                                             >  1:988212/1‑94 (MQ=255)
                                                                               aTCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGt                                                                                             <  2:988212/94‑1 (MQ=255)
                                                                                        tGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCga                                          >  1:1055802/1‑136 (MQ=255)
                                                                                        tGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCga                                          <  2:1055802/136‑1 (MQ=255)
                                                                                          cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGc                                                                               <  1:334454/97‑1 (MQ=255)
                                                                                          cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGc                                                                               >  2:334454/1‑97 (MQ=255)
                                                                                          cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg                                                                   >  1:1673419/1‑109 (MQ=255)
                                                                                          cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg                                                                   <  2:1673419/109‑1 (MQ=255)
                                                                                          cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt                                     >  1:396694/1‑139 (MQ=255)
                                                                                          cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt                                     >  2:575212/1‑139 (MQ=255)
                                                                                          cATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt                                     <  1:1956187/139‑1 (MQ=255)
                                                                                              aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACgggg                                                                                                            <  2:1932450/64‑1 (MQ=255)
                                                                                              aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACgggg                                                                                                            >  1:1932450/1‑64 (MQ=255)
                                                                                              aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAcc                                                                     >  2:1271566/1‑103 (MQ=255)
                                                                                              aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAAcc                                                                     <  1:1271566/103‑1 (MQ=255)
                                                                                              aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGt                                 >  1:1302041/1‑139 (MQ=255)
                                                                                              aGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGt                                 >  1:1850879/1‑139 (MQ=255)
                                                                                               gcgcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTc                                <  2:1780386/139‑1 (MQ=255)
                                                                                                cgcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGaa                                                                                                                              <  2:1319401/44‑1 (MQ=255)
                                                                                                cgcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGaa                                                                                                                              >  1:1319401/1‑44 (MQ=255)
                                                                                                 gcTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCt                              >  1:488963/1‑139 (MQ=255)
                                                                                                  cTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTTCTGGAATAACAGTATCCAAc                                                                                                                >  1:1590317/1‑56 (MQ=255)
                                                                                                  cTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAAc                                                                                                                <  2:1590317/56‑1 (MQ=255)
                                                                                                  cTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTc                             >  1:83528/1‑139 (MQ=255)
                                                                                                  cTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTc                             <  2:1478099/139‑1 (MQ=255)
                                                                                                   tACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCa                            <  1:1528070/139‑1 (MQ=255)
                                                                                                    aCCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCagag                                                                                        <  1:205992/78‑1 (MQ=255)
                                                                                                    aCCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCagag                                                                                        >  2:205992/1‑78 (MQ=255)
                                                                                                        gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCag                                                                                          >  1:1019397/1‑72 (MQ=255)
                                                                                                        gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCag                                                                                          <  2:1019397/72‑1 (MQ=255)
                                                                                                        gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCa                            <  2:624068/134‑1 (MQ=255)
                                                                                                        gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCa                            >  1:624068/1‑134 (MQ=255)
                                                                                                        gAGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTc                       >  2:1625489/1‑139 (MQ=255)
                                                                                                         aGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTcc                               <  1:583301/130‑1 (MQ=255)
                                                                                                         aGCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTcc                               >  2:583301/1‑130 (MQ=255)
                                                                                                          gCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt                                     >  2:233044/1‑123 (MQ=255)
                                                                                                          gCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGAtt                                     <  1:233044/123‑1 (MQ=255)
                                                                                                          gCGGGAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCaa                     >  1:1568496/1‑139 (MQ=255)
                                                                                                             ggAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg                                                                   >  2:426555/1‑90 (MQ=255)
                                                                                                             ggAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCcg                                                                   <  1:426555/90‑1 (MQ=255)
                                                                                                             ggAAAGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAgg                  >  2:819419/1‑139 (MQ=255)
                                                                                                               aaaGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCaa                                                                                                                 >  2:454511/1‑42 (MQ=255)
                                                                                                               aaaGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCaa                                                                                                                 <  1:454511/42‑1 (MQ=255)
                                                                                                               aaaGCCAGAAAAGATCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCa                >  2:712117/1‑139 (MQ=255)
                                                                                                                            aTCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGt   >  2:1863483/1‑139 (MQ=255)
                                                                                                                             tCGGTGGTGCTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTg  >  1:1734435/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                  
TTTCGGGCGGCCAGTGAAGGTGTGCCACAGGGCGGTGTTATATCGCCGCTATTATCGAACATCATGCTGAATGAGTTCGATCAATACCTGCATGAGCGCTACCTGAGCGGGAAAGCCAGAAAAGATCGGTGGTACTGGAATAACAGTATCCAACGGGGCCGAAGTACGGCGGTCAGAGAAAACTGGCAGTGGAAACCCGCGGTGGCGTACTGCCGCTATGCCGATGATTTTGTCCTCATCGTCAAAGGCACCAAAGCACAGGTG  >  NC_000913/273474‑273737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: