Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,411	C→T	*394* (TAG→TAA)	yqeF ←	short chain acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,411	0	C	T	100.0%	199.1 / NA	63	*394* (TAG→TAA)	yqeF	short chain acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/28);  total (35/28)

TCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATC  >  NC_000913/2984284‑2984546                                                                                                                                |                                                                                                                                        tCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACg                                                                                                                              >  2:450230/1‑139 (MQ=255)    aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTc                                                                                                                           >  2:700626/1‑139 (MQ=255)    aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTc                                                                                                                           >  2:614875/1‑139 (MQ=255)    aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTc                                                                                                                           >  1:621765/1‑139 (MQ=255)         gAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATgg                                                                                                                      >  2:963960/1‑139 (MQ=255)                 cATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                              <  1:614875/139‑1 (MQ=255)                   tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCcac                                                                                                            >  1:107515/1‑139 (MQ=255)                         tGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTg                                                                                                      >  1:1728603/1‑139 (MQ=255)                          ggAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                              <  1:1650822/130‑1 (MQ=255)                          ggAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGcc                                                                                                              >  2:1650822/1‑130 (MQ=255)                                 tGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgc                                                                                              >  1:649455/1‑139 (MQ=255)                                  gAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgcc                                                                                             <  2:621765/139‑1 (MQ=255)                                         cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCATATATTTATCGGTAAGGAGGAAATGCAGAGTTAt                                                                                                                                      >  1:532190/1‑91 (MQ=255)                                         cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCATATATTTATCGGTAAGGAGGAAATGCAGAGTTAt                                                                                                                                      <  2:532190/91‑1 (MQ=255)                                         cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                                   <  1:1958642/126‑1 (MQ=255)                                         cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                                   >  2:1958642/1‑126 (MQ=255)                                            aGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                                   <  1:700626/139‑1 (MQ=255)                                            aGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGc                                                                                   <  1:450230/139‑1 (MQ=255)                                                      tgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATAc                                                                                        >  2:1116883/1‑124 (MQ=255)                                                      tgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATAc                                                                                        <  1:1116883/124‑1 (MQ=255)                                                           aCTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTg                                                                    <  1:963960/139‑1 (MQ=255)                                                            cTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGc                                                                   >  1:922064/1‑139 (MQ=255)                                                            cTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGc                                                                   >  1:493486/1‑139 (MQ=255)                                                             ttGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                                  <  2:1728603/139‑1 (MQ=255)                                                              tGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCgg                                                                 >  2:596189/1‑139 (MQ=255)                                                               gccagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCggg                                                                >  1:1082349/1‑139 (MQ=255)                                                                  agccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAt                                                             <  2:107515/139‑1 (MQ=255)                                                                        catTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGtt                                                       >  1:527820/1‑139 (MQ=255)                                                                         attattCAACAGATTAATGACGGCAGATATTTATCGGTAAGGGGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                                  <  1:377244/127‑1 (MQ=255)                                                                         attattCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                             >  1:497608/1‑84 (MQ=255)                                                                         attattCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCca                                                                                                             <  2:497608/84‑1 (MQ=255)                                                                         attattCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCg                                                                  >  2:377244/1‑127 (MQ=255)                                                                                 aCAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCAttt                                              >  2:1551534/1‑139 (MQ=255)                                                                                  cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgcc                                                                                             >  2:308552/1‑91 (MQ=255)                                                                                  cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgcc                                                                                             <  1:308552/91‑1 (MQ=255)                                                                                  cAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTc                                             >  1:1876354/1‑139 (MQ=255)                                                                                   aGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                            <  2:649455/139‑1 (MQ=255)                                                                                     aTTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATg                                          >  1:1858338/1‑139 (MQ=255)                                                                                      ttAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGa                                         <  2:922064/139‑1 (MQ=255)                                                                                       tAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                                   >  1:1536780/1‑80 (MQ=255)                                                                                       tAATGACGGAAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                                                                                   <  2:1536780/80‑1 (MQ=255)                                                                                            aCGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCaga                                   <  1:1551534/139‑1 (MQ=255)                                                                                            aCGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCaga                                   <  2:1082349/139‑1 (MQ=255)                                                                                               gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                            >  1:767207/1‑127 (MQ=255)                                                                                               gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                            <  2:767207/127‑1 (MQ=255)                                                                                               gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa                                >  2:1483111/1‑139 (MQ=255)                                                                                               gCAGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGaa                                >  1:723156/1‑139 (MQ=255)                                                                                                 aGATATTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAAc                              <  1:596189/139‑1 (MQ=255)                                                                                                   atatTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGc                                                                                                               >  2:858923/1‑56 (MQ=255)                                                                                                   atatTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGc                                                                                                               <  1:858923/56‑1 (MQ=255)                                                                                                   atatTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                            <  1:1999984/123‑1 (MQ=255)                                                                                                   atatTTATCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCa                                            >  2:1999984/1‑123 (MQ=255)                                                                                                         aTCGGTAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTc                      >  2:1061433/1‑139 (MQ=255)                                                                                                              tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACAcc                                                                                                         >  2:1455009/1‑51 (MQ=255)                                                                                                              tAAGGAGGAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACAcc                                                                                                         <  1:1455009/51‑1 (MQ=255)                                                                                                                aggaggAAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAAcc               >  2:1942550/1‑139 (MQ=255)                                                                                                                     gaAATGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAg          <  2:1858338/139‑1 (MQ=255)                                                                                                                       aaTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGcc                                                                             >  2:1039949/1‑70 (MQ=255)                                                                                                                       aaTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGcc                                                                             <  1:1039949/70‑1 (MQ=255)                                                                                                                       aaTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCAcc                                                  <  2:323420/97‑1 (MQ=255)                                                                                                                       aaTGCAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCAcc                                                  >  1:323420/1‑97 (MQ=255)                                                                                                                           cAGAGTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTa    <  1:1061433/139‑1 (MQ=255)                                                                                                                             gagTTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATc  >  1:90407/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                        TCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATC  >  NC_000913/2984284‑2984546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑