Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,083,620 | G→A | R60C (CGT→TGT) | speB ← | agmatinase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,083,620 | 0 | G | A | 100.0% | 245.6 / NA | 75 | R60C (CGT→TGT) | speB | agmatinase |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (38/37); total (38/37) |
TCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGG > NC_000913/3083490‑3083751 | tCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTg > 1:1095757/1‑139 (MQ=255) gCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGac < 1:1757003/139‑1 (MQ=255) tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGaccac > 1:1148491/1‑139 (MQ=255) tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGaccac > 2:916660/1‑139 (MQ=255) tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGaccac > 2:1528831/1‑139 (MQ=255) aGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTg > 2:839433/1‑124 (MQ=255) aGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTg < 1:839433/124‑1 (MQ=255) tataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc > 1:278692/1‑131 (MQ=255) tataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc < 2:278692/131‑1 (MQ=255) gATCGCCGCAGTCCATGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGAt < 1:618480/97‑1 (MQ=255) gATCGCCGCAGTCCATGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGAt > 2:618480/1‑97 (MQ=255) gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAg < 1:1004350/135‑1 (MQ=255) gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAg > 2:1004350/1‑135 (MQ=255) gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGc > 2:1475896/1‑139 (MQ=255) aTCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc > 2:171412/1‑123 (MQ=255) aTCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc < 1:171412/123‑1 (MQ=255) ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGAt < 2:1951513/92‑1 (MQ=255) ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGAt > 1:1951513/1‑92 (MQ=255) ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGacc < 2:1938492/113‑1 (MQ=255) ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGacc > 1:1938492/1‑113 (MQ=255) ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCatat > 1:1376288/1‑139 (MQ=255) ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCatat > 2:724896/1‑139 (MQ=255) cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc > 2:1911723/1‑123 (MQ=255) cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc < 1:1911723/123‑1 (MQ=255) cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATc > 1:427344/1‑139 (MQ=255) aGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa < 1:724896/139‑1 (MQ=255) tCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGAt > 2:949784/1‑86 (MQ=255) tCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGAt < 1:949784/86‑1 (MQ=255) acgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGc < 2:427344/139‑1 (MQ=255) tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc > 1:1227868/1‑136 (MQ=255) tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc < 2:1227868/136‑1 (MQ=255) tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGt > 1:1552756/1‑139 (MQ=255) cAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa < 2:593371/139‑1 (MQ=255) cTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGt < 1:214356/132‑1 (MQ=255) cTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGt > 2:214356/1‑132 (MQ=255) tCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGacca > 1:2018688/1‑90 (MQ=255) tCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGacca < 2:2018688/90‑1 (MQ=255) tCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAccc < 2:1880872/139‑1 (MQ=255) tCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAccc < 2:1376288/139‑1 (MQ=255) cACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCa > 1:1734695/1‑139 (MQ=255) gTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCa > 2:1169385/1‑139 (MQ=255) aaaTTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGc < 1:1169385/139‑1 (MQ=255) cACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGAGAGATCGCTGCCGGACCGTGGCGACCACGCGCACGACCAGa > 1:1187976/1‑86 (MQ=255) cACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa < 2:1187976/86‑1 (MQ=255) aCGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATa > 1:1715484/1‑139 (MQ=255) cGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATAc < 2:1552756/139‑1 (MQ=255) aGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGa < 2:759714/53‑1 (MQ=255) aGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGa < 2:797089/53‑1 (MQ=255) aGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGa > 1:797089/1‑53 (MQ=255) aGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGa > 1:759714/1‑53 (MQ=255) aGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCg < 1:1922285/61‑1 (MQ=255) aGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCg > 2:1922285/1‑61 (MQ=255) cGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCg < 2:5077/90‑1 (MQ=255) cGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCg > 1:5077/1‑90 (MQ=255) gTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGt < 2:1735421/113‑1 (MQ=255) gTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGt > 1:1735421/1‑113 (MQ=255) tgtTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc < 1:549823/67‑1 (MQ=255) tgtTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc > 2:549823/1‑67 (MQ=255) aGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa < 1:1695654/79‑1 (MQ=255) aGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa > 2:1695654/1‑79 (MQ=255) ggCCAGATTCGTCGAAACCTGACATATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAgg < 2:1148491/139‑1 (MQ=255) gCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc > 1:1368810/1‑53 (MQ=255) gCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGc < 2:1368810/53‑1 (MQ=255) ccAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa < 2:1862275/61‑1 (MQ=255) ccAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa > 1:1862275/1‑61 (MQ=255) ccAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAcc > 1:1636753/1‑95 (MQ=255) ccAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAcc < 2:1636753/95‑1 (MQ=255) aTTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACTCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGc > 2:516370/1‑130 (MQ=255) aTTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACTCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGc < 1:516370/130‑1 (MQ=255) tcgAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc > 1:193850/1‑48 (MQ=255) tcgAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc < 2:193850/48‑1 (MQ=255) tcgAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAAcc > 2:918152/1‑139 (MQ=255) gAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCaa < 1:918152/139‑1 (MQ=255) aCCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAgg < 1:1475896/139‑1 (MQ=255) aCCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAgg < 1:1528831/139‑1 (MQ=255) | TCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGG > NC_000913/3083490‑3083751 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |