Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,191,696	G→A	*355* (TAG→TAA)	yqiI →	fimbrial protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,191,696	0	G	A	100.0%	122.1 / NA	39	*355* (TAG→TAA)	yqiI	fimbrial protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/21);  total (18/21)

ATTTGCTTCTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCA  >  NC_000913/3191561‑3191826                                                                                                                                        |                                                                                                                                   aTTTGCTTCTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAatat                                                                                                                                 <  2:1762045/139‑1 (MQ=255)         cttTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                         <  1:1107648/139‑1 (MQ=255)           ttttCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCTGCAGAAACGGTTGTTGAGGGACCGATTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACaaa                                                                                                                       <  2:1260840/139‑1 (MQ=255)                    tCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                         >  2:912227/1‑128 (MQ=255)                    tCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                         <  1:1391682/128‑1 (MQ=255)                    tCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                         >  2:1391682/1‑128 (MQ=255)                    tCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                         <  1:912227/128‑1 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                         <  2:434210/86‑1 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                                         >  1:434210/1‑86 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCa                                                                                       >  2:122400/1‑120 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCa                                                                                       <  1:122400/120‑1 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctct                                                                    >  2:427781/1‑139 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctct                                                                    >  1:824724/1‑139 (MQ=255)                                                                   gAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTc                                                                           >  1:471022/1‑127 (MQ=255)                                                                   gAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTc                                                                           <  2:471022/127‑1 (MQ=255)                                                                          gCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcatc                                                        <  2:877456/139‑1 (MQ=255)                                                                            aGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcatcat                                                      <  2:60269/139‑1 (MQ=255)                                                                                         gAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc                                         <  1:427781/139‑1 (MQ=255)                                                                                         gAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCCAATGCAAACACTACAACGGGCCATCAGGCCCGATATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc                                         >  1:1039873/1‑139 (MQ=255)                                                                                              aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCg                                                                                               <  2:1483379/80‑1 (MQ=255)                                                                                              aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCg                                                                                               >  1:1483379/1‑80 (MQ=255)                                                                                              aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGc                                    >  1:839926/1‑139 (MQ=255)                                                                                                          aGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctctct                                                                  <  1:265833/97‑1 (MQ=255)                                                                                                          aGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctctct                                                                  >  2:265833/1‑97 (MQ=255)                                                                                                                 aTCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAg                 <  2:839926/139‑1 (MQ=255)                                                                                                                     tGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCAt                                                                                                       >  1:337628/1‑49 (MQ=255)                                                                                                                     tGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCAt                                                                                                       <  2:337628/49‑1 (MQ=255)                                                                                                                      gAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctctct                                                                  >  1:401056/1‑85 (MQ=255)                                                                                                                      gAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctctct                                                                  <  2:401056/85‑1 (MQ=255)                                                                                                                       aaaaTCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcatc                                                        >  2:503794/1‑94 (MQ=255)                                                                                                                       aaaaTCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcatc                                                        <  1:503794/94‑1 (MQ=255)                                                                                                                        aaaTCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc                                         >  2:1651339/1‑108 (MQ=255)                                                                                                                        aaaTCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc                                         <  1:1651339/108‑1 (MQ=255)                                                                                                                            cACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCa                                                                         >  2:1766035/1‑72 (MQ=255)                                                                                                                            cACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCa                                                                         <  1:1766035/72‑1 (MQ=255)                                                                                                                              ccTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATc                                                                                        <  1:88351/55‑1 (MQ=255)                                                                                                                              ccTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATc                                                                                        >  2:88351/1‑55 (MQ=255)                                                                                                                                tataACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCa  <  2:1555531/139‑1 (MQ=255)                                                                                                                                 ataACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGttct     >  2:1098299/1‑135 (MQ=255)                                                                                                                                 ataACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGttct     <  1:1098299/135‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   ATTTGCTTCTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCA  >  NC_000913/3191561‑3191826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑