Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,205,324	C→T	*311* (TAG→TAA)	ttdR ←	transcriptional activator of ttdABT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,205,324	0	C	T	100.0%	180.3 / NA	56	*311* (TAG→TAA)	ttdR	transcriptional activator of ttdABT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

GGTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGC  >  NC_000913/3205188‑3205454                                                                                                                                         |                                                                                                                                   ggTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTa                                                                                                                                  <  2:1583336/139‑1 (MQ=255)                       aTCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCg                                                                                                            >  2:635280/1‑139 (MQ=255)                        tCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCgg                                                                                                           >  1:598606/1‑139 (MQ=255)                            gctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGCGGATTTACATGACCTGATAGCCTTCATCGGGCtt                                                                                                       >  2:10959/1‑139 (MQ=255)                             ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATg                                                                                                                              >  2:1920181/1‑115 (MQ=255)                             ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATg                                                                                                                              <  1:1920181/115‑1 (MQ=255)                             ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTg                                                                                                      >  2:1590495/1‑139 (MQ=255)                             ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTg                                                                                                      >  1:1788357/1‑139 (MQ=255)                              tgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGc                                                                                                     <  2:1788357/139‑1 (MQ=255)                                  tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                 >  1:1594189/1‑139 (MQ=255)                                    atcatGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc                                                                                               <  1:680567/139‑1 (MQ=255)                                    atcatGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc                                                                                               <  1:635280/139‑1 (MQ=255)                                       atGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCTTTCATCGGGCTTGCCCAGCCGtt                                                                                            >  2:1854145/1‑139 (MQ=255)                                         gACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCTTTCATCGGGCTTGCCCAGCCGTTGc                                                                                          <  1:1854145/139‑1 (MQ=255)                                                   aaCGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATg                                                                                <  1:1590495/139‑1 (MQ=255)                                                     cGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTg                                                                                                                         >  2:2033061/1‑96 (MQ=255)                                                     cGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTg                                                                                                                         <  1:2033061/96‑1 (MQ=255)                                                     cGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATgcc                                                                              <  2:969953/139‑1 (MQ=255)                                                         tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAGAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCa                                                                          >  1:1454845/1‑139 (MQ=255)                                                         tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCa                                                                          >  2:1602858/1‑139 (MQ=255)                                                                  gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATgccgcc                                                                           >  2:1751053/1‑129 (MQ=255)                                                                  gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATgccgcc                                                                           <  1:1751053/129‑1 (MQ=255)                                                                   tcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCt                                                                                         <  1:1965655/114‑1 (MQ=255)                                                                   tcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCt                                                                                         >  2:1965655/1‑114 (MQ=255)                                                                    cAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACg                                                               <  2:1734479/139‑1 (MQ=255)                                                                       gagaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAg                                                            >  1:538951/1‑139 (MQ=255)                                                                                   tGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTc                                                >  2:1167934/1‑139 (MQ=255)                                                                                      aCGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                             <  1:10959/139‑1 (MQ=255)                                                                                      aCGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                             >  2:1833041/1‑139 (MQ=255)                                                                                          aaTTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                <  1:1718453/52‑1 (MQ=255)                                                                                          aaTTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACa                                                                                                                                >  2:1718453/1‑52 (MQ=255)                                                                                                 aagaaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATagag                                  <  2:1594189/139‑1 (MQ=255)                                                                                                    aaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCgg                               >  2:120322/1‑139 (MQ=255)                                                                                                      agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGc                                                                                                         <  1:448900/63‑1 (MQ=255)                                                                                                      agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGc                                                                                                         >  2:448900/1‑63 (MQ=255)                                                                                                      agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTTCTGGCACCATgc                                                                               >  1:1469197/1‑89 (MQ=255)                                                                                                      agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATgc                                                                               <  2:1469197/89‑1 (MQ=255)                                                                                                      agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATg                                                                                >  1:1949157/1‑88 (MQ=255)                                                                                                      agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATg                                                                                <  2:1949157/88‑1 (MQ=255)                                                                                                      agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCt                             >  1:1445032/1‑139 (MQ=255)                                                                                                      agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCt                             >  2:1943220/1‑139 (MQ=255)                                                                                                        cgcgAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCTGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTcc                           >  2:2052169/1‑139 (MQ=255)                                                                                                               aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                    <  2:1570188/139‑1 (MQ=255)                                                                                                               aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                    <  1:1167934/139‑1 (MQ=255)                                                                                                               aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                    <  2:538951/139‑1 (MQ=255)                                                                                                               aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                    <  2:1454845/139‑1 (MQ=255)                                                                                                                        aGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATaga                                   >  1:1111479/1‑115 (MQ=255)                                                                                                                        aGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATaga                                   <  2:1111479/115‑1 (MQ=255)                                                                                                                         gTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGat          >  1:1957112/1‑139 (MQ=255)                                                                                                                           gATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGatat        >  2:1582776/1‑139 (MQ=255)                                                                                                                               tCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                             >  1:1279248/1‑98 (MQ=255)                                                                                                                               tCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGgc    <  1:2052169/139‑1 (MQ=255)                                                                                                                               tCTGGTGGATTTACATGACCTGATAGCCTGCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCAtg                                             <  2:1279248/98‑1 (MQ=255)                                                                                                                                cTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAAC‑GCCCAGATATTGgcgc  <  2:598606/139‑1 (MQ=255)                                                                                                                                 tggtggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGgcgc  <  1:1602858/139‑1 (MQ=255)                                                                                                                                 tggtggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGgcgc  <  1:1833041/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   GGTTTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACAGCCCAGATATTGGCGC  >  NC_000913/3205188‑3205454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑