Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,297,157	T→C	T328A (ACA→GCA)	yraQ ←	putative inner membrane permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,297,157	0	T	C	100.0%	138.2 / NA	39	T328A (ACA→GCA)	yraQ	putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/20);  total (19/20)

TACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAC  >  NC_000913/3297033‑3297286                                                                                                                             |                                                                                                                                  tACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCtt                                                                                                                     <  2:713696/139‑1 (MQ=255)   cAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTc                                                                                                                   >  1:1289290/1‑139 (MQ=255)                  tAGGTCAGATAAGGCGTTTACGCCGCATCCGACATG‑TTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgc                                                                                                   <  2:142401/139‑1 (MQ=255)                    ggTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgcg                                                                                                  <  1:1957741/139‑1 (MQ=255)                                       gccgcATCCGACATGTTTTCCCTCAAATCAGAACAACCGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGc                                                                               <  1:1312697/139‑1 (MQ=255)                                         cgcATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCaa                                                                             >  2:34848/1‑139 (MQ=255)                                            aTCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGc                                                                                                                 >  1:975753/1‑100 (MQ=255)                                            aTCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGc                                                                                                                 <  2:975753/100‑1 (MQ=255)                                                 aCATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTg                                                                     >  1:1679047/1‑139 (MQ=255)                                                     gTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATa                                                                                                                           <  1:1008141/81‑1 (MQ=255)                                                     gTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATa                                                                                                                           >  2:1008141/1‑81 (MQ=255)                                                       tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  1:34848/139‑1 (MQ=255)                                                       tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  2:1289290/139‑1 (MQ=255)                                                       tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  1:666620/139‑1 (MQ=255)                                                       tttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  1:972994/139‑1 (MQ=255)                                                        ttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCgcagc                                                                                              >  1:559320/1‑107 (MQ=255)                                                        ttCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCgcagc                                                                                              <  2:559320/107‑1 (MQ=255)                                                                     gAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               >  2:322570/1‑125 (MQ=255)                                                                     gAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGa                                                               <  1:322570/125‑1 (MQ=255)                                                                      aacaacAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATg                                                >  1:1990217/1‑139 (MQ=255)                                                                      aacaacAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACACTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATg                                                >  1:1216268/1‑139 (MQ=255)                                                                           caGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAgc                                           >  1:979034/1‑139 (MQ=255)                                                                           caGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAgc                                           >  1:1624525/1‑139 (MQ=255)                                                                                ccAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgcgc                                                                                                 >  1:1293962/1‑80 (MQ=255)                                                                                ccAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTgcgc                                                                                                 <  2:1293962/80‑1 (MQ=255)                                                                                   gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATATGGCTTTCGCCGGGa                                                                                                            <  2:1249229/66‑1 (MQ=255)                                                                                   gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGa                                                                                                            >  1:1249229/1‑66 (MQ=255)                                                                                   gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGc                                                             >  1:752269/1‑113 (MQ=255)                                                                                   gCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGc                                                             <  2:752269/113‑1 (MQ=255)                                                                                    cccGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCg                                  <  2:1624525/139‑1 (MQ=255)                                                                                    cccGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCg                                  <  2:168475/139‑1 (MQ=255)                                                                                                  cacCAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGc                    >  1:1257973/1‑139 (MQ=255)                                                                                                    ccAGACACTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCa                  <  1:572462/139‑1 (MQ=255)                                                                                                         cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCa                           >  1:189865/1‑125 (MQ=255)                                                                                                         cacTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCa                           <  2:189865/125‑1 (MQ=255)                                                                                                          acTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGCTCCCATACCTGCCAGcatca            >  1:902897/1‑139 (MQ=255)                                                                                                           cTGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGcatcat           <  2:1216268/139‑1 (MQ=255)                                                                                                            tGCCACCATCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCatcatc          >  1:1412122/1‑139 (MQ=255)                                                                                                                    tCGCCCCTGCCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAc  >  1:567406/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                  TACAACGATACAAACCGTAGGTCAGATAAGGCGTTTACGCCGCATCCGACATGTTTTCCCTCAAATCAGAACAACAGCGCCAGCCCGCCGACAATCACACCAGACACTGCCACCATCGCCCCTGTCAGCCATAAGGCTTTCGCCGGGAACGCTTTGCGCAGCATAATCAGTGACGGCAAACTCACTGCCGGGAGCGTCATCAACAATGCCAGCGCCGGAGCGGTTCCCATACCTGCCAGCATCATCGTTTGTAC  >  NC_000913/3297033‑3297286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑