Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,298,035 T→C E35G (GAA→GGA)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,298,0350TC100.0% 353.0 / NA 98E35G (GAA→GGA) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (47/51);  total (47/51)

Only 100 of 107 total aligned reads displayed.

CTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTT  >  NC_000913/3297901‑3298159
                                                                                                                                      |                                                                                                                            
cTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          <  1:388318/139‑1 (MQ=255)
cTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          <  2:2050326/139‑1 (MQ=255)
cTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          <  1:1359916/139‑1 (MQ=255)
            cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTcac                                                                                                                    >  1:1360831/1‑133 (MQ=255)
            cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTcac                                                                                                                    <  2:1360831/133‑1 (MQ=255)
            cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCa                                                                                                              >  1:48775/1‑139 (MQ=255)
                gAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAgaga                                                                                                          <  2:48775/139‑1 (MQ=255)
                gAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAgaga                                                                                                          <  1:461445/139‑1 (MQ=255)
                      gATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCa                                                                                                    <  1:1205091/139‑1 (MQ=255)
                         catcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTcccc                                                                                                                           <  1:1039207/113‑1 (MQ=255)
                         catcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTcccc                                                                                                                           >  2:1039207/1‑113 (MQ=255)
                          atcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGa                                                                                                >  2:367972/1‑139 (MQ=255)
                           tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGAc                                                                                               >  1:1584898/1‑139 (MQ=255)
                           tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGAc                                                                                               >  2:1404703/1‑139 (MQ=255)
                            cgcgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                              >  2:876452/1‑139 (MQ=255)
                            cgcgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                              >  2:1998652/1‑139 (MQ=255)
                              cgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAAc                                                                                            >  1:224442/1‑139 (MQ=255)
                                 aaTCCAACGCCGCCTGCCAAGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACgag                                                                                         >  2:1369647/1‑139 (MQ=255)
                                   tCCAACGCCGCCTGTCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          <  2:1658272/104‑1 (MQ=255)
                                   tCCAACGCCGCCTGTCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          >  1:1658272/1‑104 (MQ=255)
                                          ccgccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAg                                                                                >  1:1740684/1‑139 (MQ=255)
                                           cgccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTATACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgc                                                                               >  2:166130/1‑139 (MQ=255)
                                             ccTGCCATGGGTTAGCATCCGCCTGTGCTAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACgaga                                                                                        <  1:102724/128‑1 (MQ=255)
                                             ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGCCCGGCAATGACAACgaga                                                                                        >  2:102724/1‑128 (MQ=255)
                                             ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             <  1:433393/139‑1 (MQ=255)
                                             ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             <  1:876452/139‑1 (MQ=255)
                                             ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             >  1:2026000/1‑139 (MQ=255)
                                             ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                              >  1:1525704/1‑138 (MQ=255)
                                             ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                              <  2:1525704/138‑1 (MQ=255)
                                             ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCAGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             >  2:654691/1‑139 (MQ=255)
                                                  cATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGtt                                                                        <  2:224442/139‑1 (MQ=255)
                                                    tGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTc                                                                      <  1:166130/139‑1 (MQ=255)
                                                       gTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccac                                                                   <  2:541639/139‑1 (MQ=255)
                                                         tAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacca                                                                 <  2:1689770/139‑1 (MQ=255)
                                                         tAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacca                                                                 <  2:460540/139‑1 (MQ=255)
                                                         tAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGCGTTTccacca                                                                 <  1:1369647/139‑1 (MQ=255)
                                                           gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCAtt                                                                                                                        <  2:1405465/82‑1 (MQ=255)
                                                           gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCAtt                                                                                                                        >  1:1405465/1‑82 (MQ=255)
                                                           gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                              >  1:216759/1‑124 (MQ=255)
                                                           gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                              <  2:216759/124‑1 (MQ=255)
                                                           gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACt                                                               >  1:1761979/1‑139 (MQ=255)
                                                                cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATg                                                          >  1:1865603/1‑139 (MQ=255)
                                                                        cAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTcccc                                                                                                                           >  1:1632557/1‑66 (MQ=255)
                                                                           ggATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTgccgc                                               <  1:367972/139‑1 (MQ=255)
                                                                             aTAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                             <  2:1740684/139‑1 (MQ=255)
                                                                             aTAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                             <  1:1998652/139‑1 (MQ=255)
                                                                             aTAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                             <  2:1584898/139‑1 (MQ=255)
                                                                                gATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTcccc                                                                                                                           >  2:1131203/1‑58 (MQ=255)
                                                                                gATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTcccc                                                                                                                           <  1:1131203/58‑1 (MQ=255)
                                                                                gATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACga                                                                                          <  2:1456158/91‑1 (MQ=255)
                                                                                gATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACga                                                                                          >  1:1456158/1‑91 (MQ=255)
                                                                                    tACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGa                                      <  1:754055/139‑1 (MQ=255)
                                                                                     aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCaga                                                                                                            >  1:1173506/1‑68 (MQ=255)
                                                                                     aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGaa                                     <  1:336636/139‑1 (MQ=255)
                                                                                     aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGaa                                     >  1:1809726/1‑139 (MQ=255)
                                                                                      ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTccc                                                                                                                            >  1:1294327/1‑51 (MQ=255)
                                                                                      ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAg                                    >  2:1642013/1‑139 (MQ=255)
                                                                                          tACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTg                                >  1:863813/1‑139 (MQ=255)
                                                                                           aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTcccc                                                                                                                           <  1:767745/47‑1 (MQ=255)
                                                                                           aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTcccc                                                                                                                           >  2:767745/1‑47 (MQ=255)
                                                                                            cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  2:1236956/139‑1 (MQ=255)
                                                                                            cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  2:863813/139‑1 (MQ=255)
                                                                                               tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCgg                                                                                                      >  2:1085479/1‑64 (MQ=255)
                                                                                               tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCgg                                                                                                      <  1:1085479/64‑1 (MQ=255)
                                                                                               tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCg                                                                                                       <  2:592200/63‑1 (MQ=255)
                                                                                               tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                              >  1:1909209/1‑88 (MQ=255)
                                                                                               tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                              <  2:1909209/88‑1 (MQ=255)
                                                                                               tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAg                           >  2:1508985/1‑139 (MQ=255)
                                                                                               tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAg                           >  2:433928/1‑139 (MQ=255)
                                                                                               tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAg                           >  2:711749/1‑139 (MQ=255)
                                                                                                gggTTTCGGCAGCAGTAAACGCTTTGCCGT‑GTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             >  1:955988/1‑87 (MQ=255)
                                                                                                 ggTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGAc                                                                                               <  1:853942/69‑1 (MQ=255)
                                                                                                 ggTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGAc                                                                                               >  2:853942/1‑69 (MQ=255)
                                                                                                    ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                           >  2:1215734/1‑86 (MQ=255)
                                                                                                    ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTcca                                                                    >  2:229907/1‑93 (MQ=255)
                                                                                                    ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTcca                                                                    <  1:229907/93‑1 (MQ=255)
                                                                                                    ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATa                      >  2:335932/1‑139 (MQ=255)
                                                                                                    ttCGCCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                           <  1:1215734/86‑1 (MQ=255)
                                                                                                      cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGAcc                                                                                                        <  1:1228259/55‑1 (MQ=255)
                                                                                                      cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGAcc                                                                                                        >  2:1228259/1‑55 (MQ=255)
                                                                                                      cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  2:122271/129‑1 (MQ=255)
                                                                                                      cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              >  1:122271/1‑129 (MQ=255)
                                                                                                       ggCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                   <  1:433928/139‑1 (MQ=255)
                                                                                                            cagTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTccc              >  2:187800/1‑139 (MQ=255)
                                                                                                             agTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacca                                                                 >  2:605448/1‑87 (MQ=255)
                                                                                                             agTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacca                                                                 <  1:605448/87‑1 (MQ=255)
                                                                                                                  aCGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGaa        <  1:1508985/139‑1 (MQ=255)
                                                                                                                      tttGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              >  1:84787/1‑113 (MQ=255)
                                                                                                                      tttGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  2:84787/113‑1 (MQ=255)
                                                                                                                       ttGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                   >  1:103332/1‑107 (MQ=255)
                                                                                                                       ttGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                   <  2:103332/107‑1 (MQ=255)
                                                                                                                       ttGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt   <  1:1642013/139‑1 (MQ=255)
                                                                                                                       ttGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt   <  2:2026000/139‑1 (MQ=255)
                                                                                                                        tGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGt                                                                         >  1:1070702/1‑68 (MQ=255)
                                                                                                                        tGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGt                                                                         <  2:1070702/68‑1 (MQ=255)
                                                                                                                        tGCCGTAGTAAGGTCCCCATTTCACATACCAGAAACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGCTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCATGAATAAGtt  >  1:74703/1‑139 (MQ=255)
                                                                                                                                  aGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                               <  1:1497258/100‑1 (MQ=255)
                                                                                                                                  aGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                               >  2:1497258/1‑100 (MQ=255)
                                                                                                                                  aGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              >  2:1468708/1‑101 (MQ=255)
                                                                                                                                  aGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  1:1468708/101‑1 (MQ=255)
                                                                                                                                      |                                                                                                                            
CTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTT  >  NC_000913/3297901‑3298159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑