Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,308,868	G→A	C19C (TGC→TGT)	nlpI ←	lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,308,868	0	G	A	100.0%	161.3 / NA	50	C19C (TGC→TGT)	nlpI	lipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/27);  total (23/27)

AGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCC  >  NC_000913/3308734‑3309003                                                                                                                                         |                                                                                                                                        aGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATc                                                                                                                                     <  2:1152917/139‑1 (MQ=255)     gtgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACa                                                                                                                                       <  2:104848/133‑1 (MQ=255)     gtgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGc                                                                                                                                 >  2:167271/1‑139 (MQ=255)      tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGTCCGGAA‑‑GTATTACTACATCCTGCa                                                                                                                                >  2:1150816/1‑139 (MQ=255)      tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCa                                                                                                                                >  2:152177/1‑139 (MQ=255)      tgCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCa                                                                                                                                <  2:1346759/139‑1 (MQ=255)                      gTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTCCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCg                                                                                                                <  2:1358854/139‑1 (MQ=255)                       ttAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               >  2:588398/1‑139 (MQ=255)                       ttAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               <  1:335487/139‑1 (MQ=255)                                gACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               <  2:175607/130‑1 (MQ=255)                                gACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               >  1:175607/1‑130 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCt                                                                                                                                   >  2:850896/1‑109 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCt                                                                                                                                   <  1:850896/109‑1 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGc                                                                                                                                 <  1:1536083/111‑1 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGc                                                                                                                                 >  2:1536083/1‑111 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGt                                                                                                                  <  2:1025838/126‑1 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGt                                                                                                                  >  1:1025838/1‑126 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAacac                                                                                                     >  1:625234/1‑139 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAacac                                                                                                     <  1:152177/139‑1 (MQ=255)                                 aCTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAacac                                                                                                     <  1:167271/139‑1 (MQ=255)                                     gCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAgc                                                                                                 <  2:967735/139‑1 (MQ=255)                                                    aTACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCa                                                                                  >  2:812484/1‑139 (MQ=255)                                                         tGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCATAAAAGGCTTCATTTcc                                                                             <  1:588398/139‑1 (MQ=255)                                                          gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTccc                                                                            >  1:94509/1‑139 (MQ=255)                                                          gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTccc                                                                            >  2:1916815/1‑139 (MQ=255)                                                          gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTccc                                                                            >  1:279536/1‑139 (MQ=255)                                                          gCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTccc                                                                            >  1:1715797/1‑139 (MQ=255)                                                           ccAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           <  2:625234/139‑1 (MQ=255)                                                           ccAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           <  2:279536/139‑1 (MQ=255)                                                                   aCTTCCTGCTGTAAAGTCGGTAGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGaa                                                                   <  1:1584206/139‑1 (MQ=255)                                                                     ttCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           <  2:145130/129‑1 (MQ=255)                                                                     ttCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           >  1:145130/1‑129 (MQ=255)                                                                       ccTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGAcc                                                               <  2:1490090/139‑1 (MQ=255)                                                                           ctgTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               >  2:534146/1‑87 (MQ=255)                                                                           ctgTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGc                                                                                                               <  1:534146/87‑1 (MQ=255)                                                                                     ggTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAAc                                                 >  2:861460/1‑139 (MQ=255)                                                                                        tGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           <  1:1059255/110‑1 (MQ=255)                                                                                        tGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCa                                                                           >  2:1059255/1‑110 (MQ=255)                                                                                             tGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTGTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTc                                              <  2:647401/136‑1 (MQ=255)                                                                                             tGGTACCGCGAGGACTTCACTTTTACGCCAGGAAGTGTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTc                                              >  1:647401/1‑136 (MQ=255)                                                                                                   cgcgAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGt                                   <  1:812484/139‑1 (MQ=255)                                                                                                       aGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCt                               >  2:1069472/1‑139 (MQ=255)                                                                                                            ttCACTTTTACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACaa                          <  1:861460/139‑1 (MQ=255)                                                                                                                   ttACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaa                                                      <  2:178131/104‑1 (MQ=255)                                                                                                                   ttACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTtgaa                                                      >  1:178131/1‑104 (MQ=255)                                                                                                                   ttACGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTACCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTcc                   >  1:1244587/1‑139 (MQ=255)                                                                                                                      cGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAg                         >  2:1779815/1‑130 (MQ=255)                                                                                                                      cGCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAg                         <  1:1779815/130‑1 (MQ=255)                                                                                                                       gCCAGGAA‑‑GTATTACTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGcc               <  2:94509/139‑1 (MQ=255)                                                                                                                                      aCTACATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGccc  <  2:1715797/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        AGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTAAAGTCGGTTGCAATGGTACCGCGAGGACTTCACTTTTACGCCAGGAA‑‑GTATTACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCC  >  NC_000913/3308734‑3309003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑