Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,254	G→A	G107G (GGC→GGT)	nanT ←	sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,254	0	G	A	100.0%	169.5 / NA	52	G107G (GGC→GGT)	nanT	sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/23);  total (29/23)

TGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCA  >  NC_000913/3372122‑3372389                                                                                                                                     |                                                                                                                                        tGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGgc                                                                                                                                   >  2:1625864/1‑139 (MQ=255)     aTCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                               <  2:1785934/139‑1 (MQ=255)               cACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGc                                                                                                                     >  2:1548738/1‑139 (MQ=255)                aCTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGcc                                                                                                                    <  2:1772536/139‑1 (MQ=255)                                    gTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagag                                                                                                >  1:991882/1‑139 (MQ=255)                                      tttGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagagaa                                                                                              <  1:1280623/139‑1 (MQ=255)                                       ttGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                               >  2:1508483/1‑105 (MQ=255)                                       ttGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                               <  1:1508483/105‑1 (MQ=255)                                       ttGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAc                                                                                             >  1:873226/1‑139 (MQ=255)                                                tttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGt                                                                                    <  2:991882/139‑1 (MQ=255)                                                         cATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc                                                                                 <  1:783386/133‑1 (MQ=255)                                                         cATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc                                                                                 >  2:783386/1‑133 (MQ=255)                                                            aGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAg                                                                        >  2:1781172/1‑139 (MQ=255)                                                              gTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgac                                                                      >  1:1725014/1‑139 (MQ=255)                                                                  cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGa                                                                                           >  1:1448330/1‑114 (MQ=255)                                                                  cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGa                                                                                           <  2:1448330/114‑1 (MQ=255)                                                                  cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGacgacg                                                                  >  1:483841/1‑139 (MQ=255)                                                                    cTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGCAGAGAACGATGCTGGTGACCATTGCCACACGACGcc                                                                >  1:610152/1‑139 (MQ=255)                                                                       gAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGt                                                             >  1:1997566/1‑139 (MQ=255)                                                                         aCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAg                                                           <  1:1781172/139‑1 (MQ=255)                                                                         aCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAg                                                           <  2:483841/139‑1 (MQ=255)                                                                         aCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAg                                                           <  2:1463919/139‑1 (MQ=255)                                                                           cGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCg                                                         <  1:1770771/139‑1 (MQ=255)                                                                               ttCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCa                                                     >  2:617531/1‑139 (MQ=255)                                                                                    ccGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGa                                                                                           >  2:1229192/1‑96 (MQ=255)                                                                                    ccGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGa                                                                                           <  1:1229192/96‑1 (MQ=255)                                                                                       ccATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCg                                                                                                                          >  2:1897369/1‑62 (MQ=255)                                                                                       ccATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCg                                                                                                                          <  1:1897369/62‑1 (MQ=255)                                                                                       ccATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAgc                                             >  2:1811767/1‑139 (MQ=255)                                                                                         aTCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCg                                                         >  1:1030994/1‑125 (MQ=255)                                                                                         aTCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCg                                                         <  2:1030994/125‑1 (MQ=255)                                                                                                tGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGcc                                                                                                                    >  2:1555654/1‑59 (MQ=255)                                                                                                tGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGcc                                                                                                                    <  1:1555654/59‑1 (MQ=255)                                                                                                tGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc                                                                                 >  2:1604287/1‑94 (MQ=255)                                                                                                tGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc                                                                                 <  1:1604287/94‑1 (MQ=255)                                                                                                tGCCGATGACCAGACGAGCGATAAACATGGTAATGTAACCTGGCGCAAAGCCGCAGGCCAGCGAACCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCAGTCACCCATAGCGCCGACCaa                                    >  1:1614660/1‑138 (MQ=255)                                                                                                     aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGt                                                       >  1:986112/1‑115 (MQ=255)                                                                                                     aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGt                                                       <  2:986112/115‑1 (MQ=255)                                                                                                     aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATa                                               >  2:1420332/1‑123 (MQ=255)                                                                                                     aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATa                                               <  1:1420332/123‑1 (MQ=255)                                                                                                      tGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgcc                              >  1:1648859/1‑139 (MQ=255)                                                                                                       gACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgccg                             <  2:1725014/139‑1 (MQ=255)                                                                                                                 gCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGc                   <  1:1811767/139‑1 (MQ=255)                                                                                                                  cGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCg                  >  2:934849/1‑139 (MQ=255)                                                                                                                   gATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                                             >  1:1714525/1‑47 (MQ=255)                                                                                                                   gATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                                             <  2:1714525/47‑1 (MQ=255)                                                                                                                       aaCATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagaga             >  2:1793708/1‑139 (MQ=255)                                                                                                                            ggTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAg        >  2:818209/1‑139 (MQ=255)                                                                                                                               gATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCg                                                         <  1:690987/87‑1 (MQ=255)                                                                                                                               gATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCg                                                         >  2:690987/1‑87 (MQ=255)                                                                                                                                 tGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGc   >  2:844303/1‑139 (MQ=255)                                                                                                                                  gTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCa  <  1:818209/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCA  >  NC_000913/3372122‑3372389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑